2017-2018年华东地区H9亚型禽流感病毒分离毒株的抗原差异分析

doi:10.11843/j.issn.0366-6964.2019.06.013

Abstract

Abstract: This experiment was conducted to understand the prevalence and variation of H9 subtype avian influenza virus (AIV) isolated in East China. Diseased samples from clinical suspected cases and swab samples from poultry in live poultry markets were collected and subjected to inoculate SPF chicken embryos. H9 AIVs were identified by HA and HI test. HA genes of partial H9 AIVs were amplified by RT-PCR, and PCR productions were subjected to sequence. Antisera from SPF chickens vaccinated with four selected strains were performed cross-reactivity by HI test to analyze the antigen variation. Results were as follows:62 isolates of H9 subtype AIV were identified, and the isolation rate was 2.02% (62/3 074). Sequence analysis of 25 H9 subtype AIVs revealed that the HA genes were belonged to the h9.4.2.5 lineage, and further were divided into Group A and Group B. The amino acid at the HA gene cleavage site was PSRSSR↓G, which is consistent with the characteristics of low pathogenic avian influenza virus. Compared with the amino acid of the HA gene of the Y280-like strain, the left arm of the receptor binding site (RBS) was changed to NGLMGR. There was a change of R (64%)/K (36%) at antigenic residue 92. The cross-reactivity of antisera showed that the HI antibody titers were differed by 2-8 log2 between the isolates and the isolates could be categorized to two antigenic phenotypes. Therefore, antigen drift is appeared in the prevalent H9N2 AIVs, and at least two antigenic phenotypes are existed simultaneously.

CLC Number:

S852.659.5

SHEN Songwei, WANG Zeyuan, FAN Weifeng, ZHU Changchun, LU You, SHI Baolan, LIU Lei, SHEN Haifeng, JIAO Kuhua, QIN Tao, CHEN Sujuan, PENG Daxin, LIU Xiufan. Antigen Difference Analysis of H9 Subtype Avian Influenza Viruses Isolated in East China during 2017-2018[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1230-1238.

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Antigen Difference Analysis of H9 Subtype Avian Influenza Viruses Isolated in East China during 2017-2018

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