山羊副流感病毒3型感染MDBK细胞的转录组分析

doi:10.11843/j.issn.0366-6964.2019.02.014

Abstract

Abstract:

To explore the cellular transcriptional gene differentially expressed in MDBK cells infected with caprine parainfluenza virus type 3 (CPIV3), we analyzed the mRNA expression profiles in MDBK cells infected with 1 MOI CPIV3 JSHA2014-1 strain at 24 hpi using high-throughput sequencing. The results indicated that a total of 261 genes were obviously differentially expressed in the infected MDBK cells including 140 up-regulated genes and 121 down-regulated genes. The eight of these genes were verified by RT-qPCR assay, and the results were consistent with those of high-throughput sequencing. GO analysis classification showed that differentially expressed genes were mainly involved in biological processes, cellular components and molecular functions. KEGG analysis shows that the signaling pathways involved in these genes included metabolism-related signaling pathways, biological systems, cellular processes, gene information processes and environmental information processes. This study laid the foundation for further exploring the pathogenesis of CPIV3.

CLC Number:

S852.659.5

ZHONG Chunyan, LI Jizong, MAO Li, LI Wenliang, HAO Fei, SUN Min, LIU Maojun, ZHU Xing, JI Xinqin, XIAO Fang, YANG Leilei, ZHANG Wenwen. Transcriptome Analysis of MDBK Cells Infected with the Caprine Parainfluenza Virus Type 3 JSHA2014-1 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 364-372.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2019.02.014

https://www.xmsyxb.com/EN/Y2019/V50/I2/364

References

[1] LI W L, MAO L, CHENG S P, et al. A novel parainfluenza virus type 3(PIV3) identified from goat herds with respiratory diseases in eastern China[J]. Veterin Microbiol, 2014, 174(1-2):100-106.
[2] YANG L L, LI W L, MAO L, et al. Analysis on the complete genome of a novel caprine parainfluenza virus 3[J]. Infect Genet Evolut, 2016, 38:29-34.
[3] LI J Z, LI W L, MAO L, et al. Rapid detection of novel caprine parainfluenza virus type 3(CPIV3) using a TaqMan-based RT-qPCR[J]. J Virol Methods, 2016, 236:126-131.
[4] COSTA V, ANGELINI C, DE FEIS I, et al. Uncovering the complexity of transcriptomes with RNA-Seq[J]. J Biomed Biotechnol, 2010, 2010:853916.
[5] CONG F, LIU X L, HAN Z X, et al. Transcriptome analysis of chicken kidney tissues following coronavirus avian infectious bronchitis virus infection[J]. BMC Genomics, 2013, 14(1):743.
[6] LI M Y, TAN H W, WANG F, et al. De Novo transcriptome sequence assembly and identification of AP2/ERF transcription factor related to abiotic stress in parsley (Petroselinum crispum)[J]. PLoS One, 2014, 9(9):e108977.
[7] ZHU S Y, TANG S W, TANG Q M, et al. Genome-wide transcriptional changes of ramie (Boehmeria nivea L. Gaud) in response to root-lesion nematode infection[J]. Gene, 2014, 552(1):67-74.
[8] 刘倩宏. 深度测序技术对羊布鲁菌16M株感染小鼠巨噬细胞转录组学的研究[D]. 长春:吉林大学, 2012.
LIU Q H. Deep sequencing-based expresssion transcriptional profiling changes of mouse macrophages during Brucella melitensis 16M infection[D]. Changchun:Jilin University, 2012. (in Chinese)
[9] 陈达香,陈瑜,郝文波,等. 羊口疮病毒作为免疫调节剂在疾病治疗中的研究进展[J]. 生物技术通讯, 2017, 28(2):182-187.
CHEN D X, CHEN Y, HAO W B, et al. Progress in Orf virus as an immunomodulator in diseases treatment[J]. Letters in Biotechnology, 2017, 28(2):182-187. (in Chinese)
[10] 李基棕,李文良,毛立,等. 山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析[J]. 畜牧兽医学报, 2017, 48(5):896-906.
LI J Z, LI W L, MAO L, et al. Identification and analysis of the miRNA expression profiles in MDBK cells in response to the infection of caprine parainfluenza virus type 3(CPIV3)[J]. Acta Veterinaria et Zootechnica Sinica, 2017, 48(5):896-906. (in Chinese)
[11] COCK P J A, FIELDS C J, GOTO N, et al. The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants[J]. Nucleic Acids Res, 2010, 38(6):1767-1771.
[12] KIM D, LANGMEAD B, SALZBERG S L. HISAT:a fast spliced aligner with low memory requirements[J]. Nat Methods, 2015, 12(4):357-360.
[13] ERLICH Y, MITRA P P, DELABASTIDE M, et al. Alta-Cyclic:a self-optimizing base caller for next-generation sequencing[J]. Nat Methods, 2008, 5(8):679-682.
[14] MORTAZAVI A, WILLIAMS B A, MCCUE K, et al. Mapping and quantifying mammalian transcriptomes by RNA-Seq[J]. Nat Methods, 2008, 5(7):621-628.
[15] ANDERS S, HUBER W. Differential expression analysis for sequence count data[J]. Genome Biol, 2010, 11(10):R106.
[16] WESTFALL P H, YOUNG S S. P value adjustments for multiple tests in multivariate binomial models[J]. J Amer Statist Assoc, 1989, 84(407):780-786.
[17] XING S S, DU J Z, GAO S D, et al. Analysis of the miRNA expression profile in an Aedes albopictus cell line in response to bluetongue virus infection[J]. Infect Genet Evolut, 2016, 39:74-84.
[18] 岳桂东,高强,罗龙海,等. 高通量测序技术在动植物研究领域中的应用[J]. 中国科学:生命科学, 2012, 42(2):107-124.
YUE G D, GAO Q, LUO L H, et al. The application of high-throughput sequencing technology in plant and animal research[J]. Scientia Sinica (Vitae), 2012, 42(2):107-124. (in Chinese)
[19] SUN Y, HAN M Y, KIM C, et al. Interplay between interferon-mediated innate immunity and porcine reproductive and respiratory syndrome virus[J]. Viruses, 2012, 4(4):424-446.
[20] UEMATSU S, SATO S, YAMAMOTO M, et al. Interleukin-1 receptor-associated kinase-1 plays an essential role for Toll-like receptor (TLR)7-and TLR9-mediated interferon-α induction[J]. J Exp Med, 2005, 201(6):915-923.
[21] MEYERS J A, MANGINI A J, NAGAI T, et al. Blockade of TLR9 agonist-induced type I interferons promotes inflammatory cytokine IFN-γ and IL-17 secretion by activated human PBMC[J]. Cytokine, 2006, 35(5-6):235-246.
[22] KAWAI T, TAKAHASHI K, SATO S, et al. IPS-1, an adaptor triggering RIG-I-and Mda5-mediated type I interferon induction[J]. Nat Immunol, 2005, 6(10):981-988.
[23] AU W C, YEOW W S, PITHA P M. Analysis of functional domains of interferon regulatory factor 7 and its association with IRF-3[J]. Virology, 2001, 280(2):273-282.
[24] RUVOLO V, NAVARRO L, SAMPLE C E, et al. The epstein-barr virus SM protein induces STAT1 and interferon-stimulated gene expression[J]. J Virol, 2003, 77(6):3690-3701.
[25] LEAMAN D W, CHAWLA-SARKAR M, JACOBS B, et al. Novel growth and death related interferon-stimulated genes (ISGs) in melanoma:greater potency of IFN-β compared with IFN-α2[J]. J Interf Cytok Res, 2003, 23(12):745-746.
[26] HARADA H, FUJITA T, MIYAMOTO M, et al. Structurally similar but functionally distinct factors, IRF-1 and IRF-2, bind to the same regulatory elements of IFN and IFN-inducible genes[J]. Cell, 1989, 58(4):729-739.
[27] SUN K, METZGER D W. Inhibition of pulmonary antibacterial defense by interferon-γ during recovery from influenza infection[J]. Nat Med, 2008, 14(5):558-564.
[28] 钟纯燕, 李基棕, 毛立,等. MiR-222对山羊副流感病毒3型复制的影响[J].畜牧兽医学报,2018,49(12):2664-2671.
ZHONG C Y, LI J Z, MAO L, et al. The molecular mechanism study of miR-222 inhibit caprine parainfluenza virus type 3 replication[J]. Acta Veterinaria et Zootechnica Sinica, 2018, 49(12):2664-2671. (in Chinese)
[29] MCSHARRY J J. Analysis of virus-infected cells by flow cytometry[J]. Methods, 2000, 21(3):249-257.
[30] FREDERICKSEN F, DELGADO F, CABRERA C, et al. The effects of reference genes in qRT-PCR assays for determining the immune response of bovine cells (MDBK) infected with the Bovine Viral Diarrhea Virus 1(BVDV-1)[J]. Gene, 2015, 569(1):95-103.
[31] ZHANG Q Z, SHI K C, YOO D. Suppression of type I interferon production by porcine epidemic diarrhea virus and degradation of CREB-binding protein by nsp1[J]. Virology, 2016, 489:252-268.
[32] CHEN J, YANG Y F, YANG Y, et al. AXL promotes Zika virus infection in astrocytes by antagonizing type I interferon signalling[J]. Nat Microbiol, 2018, 3(3):302-309.

Transcriptome Analysis of MDBK Cells Infected with the Caprine Parainfluenza Virus Type 3 JSHA2014-1 Strain

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	MENG Lingzhai, CHEN Chunli, YU Mengmeng, WANG Zhanxin, WANG Suyan, LIU Peng, HE Tana, GUO Ru, CHEN Yuntong, LIU Changjun, QI Xiaole, WU Zhiqiang, GAO Yulong. Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5154-5161.
[2]	JIANG Shengqiang, HU Jing, CHEN Hongying. Expression Analysis of CircRNAs in A549 Cells Infected with H1N1 Influenza A Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4724-4734.
[3]	ZHANG Ao, TAN Bin, LIU Kexin, LIU Jiali, ZHANG Shuqin. Genomic Characterization Analysis of a H1N1 Subtype Swine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4866-4871.
[4]	HE Chenxiang, GAO Shandian, TIAN Zhancheng, DU Junzheng, WANG Jinming, GUAN Guiquan, YIN Hong. Development of a Differential Diagnostic ELISA Based on G_NS Protein to Distinguish BEFV Infected and Vaccinated Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4320-4326.
[5]	CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.
[6]	FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207.
[7]	LI Shun, LI Guizhen, YUAN Yaoxian, LI Kangjian, MA Chunquan, LI Shoujun, HUANG Liangzong, MA Jun. Effect of the Length of PA-X Gene on Replication and Pathogenicity of H3N2 Canine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 272-280.
[8]	CUI Mingxian, WANG Xingbo, HUANG Yanming, BIAN Xiyi, FENG Mengke, YAN Yan, DONG Weiren, ZHOU Jiyong. Genetic Characterization and Evolution of Three Strains of H3N2 Avian Influenza Viruses [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4116-4122.
[9]	YU Mengmeng, BAO Yuanling, WANG Suyan, XIN Ziqi, LIU Peng, FENG Xiaoyan, MENG Lingzhai, GUO Ru, ZHANG Yanping, LIU Changjun, QI Xiaole, LI Junping, WANG Xiaomei, GAO Yulong. Isolation, Identification and Pathogenicity of Subtype B Avian Metapneumovirus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3540-3549.
[10]	ZHOU Yong, LI Zhixin, LU Hongwei, SUN Yan, LI Tian, DU Fanshu, PU Juan. Surveillance and Outbreak Analysis of H5 and H7N9 Subtypes of Highly Pathogenic Avian Influenza in China [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3093-3106.
[11]	PAN Chunrong, DENG Ruixue, HU Linjie, ZHU Xueliang, SUN Yuefeng, WANG Guirong, MENG Xuelian. Effect of Histone Deacetylase Inhibitors on Peste Des Petits Ruminants Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2307-2316.
[12]	CHEN Zixuan, ZHANG Nan, HU Qun, QUAN Keji, QIN Tao, CHEN Sujuan, PENG Daxin, LIU Xiufan. Mutation Analysis of Antigen Sites 145 and 153 on Hemagglutinin of H9N2 Avian Influenza Virus in China [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1165-1172.
[13]	DUAN Zhiqiang, XIE Lingling, CHEN Jiaqi, TANG Hong, WANG Yanbi, ZHAO Caiqin, ZHAO Jiafu. Research Progress on the Interactions of Newcastle Disease Virus M Protein with Host Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 32-42.
[14]	YAN Yayao, GU Min, LIU Xiufan. Advance in the Influence of Amino Acid Variation in HA Protein on the Biological Properties of H7N9 Subtype Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2093-2106.
[15]	JIANG Mei, CHEN Wu, ZHAI Junqiong, BU Wandi, XIE Yilun, LIU Canbin, SHAN Fen, LUO Manlin. Cloning and Sequence Analysis of H and F Genes of Canine Distemper Virus Strain from Red Panda [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1670-1676.