禽流感病毒H9N2与新城疫病毒在鸡成纤维细胞中共感染对病毒复制的影响

doi:10.11843/j.issn.0366-6964.2018.11.025

Abstract

Abstract:

The co-infection of avian influenza virus (AIV) and Newcastle disease virus (NDV) in Chickens is common clinical incident. To study the effect of co-infection of AIV (H9N2) and NDV (Herts/33) on virus replication in the DF-1 of chicken embryo fibroblasts, the replication of AIV and NDV in the DF-1 was first analyzed respectively and post-infection 12 and 24 h were selected as sample collecting time. The Western blot, immunofluorescence assay and real-time fluorescence quantitative PCR (qPCR) were conducted to compare the replication of AIV and NDV between co-infection and individual infection groups. Compared with the single virus infection group, the intensity band ratio of NDV NP in Western blot analysis in 12 and 24 h post-infection were down-regulated by 70.6% (P<0.001) and 45.7% (P<0.001),while AIV NP was down-regulated by 61.5% (P<0.001) and 82.8% (P<0.001), respectively. The optical intensity band ratio of AIV NP and NDV NP were decreased by 81.1% (P<0.001) and 65.5% (P<0.001) in 12 h post-infection, 41.2% (P<0.05) and 47.4% (P<0.001) in 24 h post-infection. The relative mRNA expression of NDV NP in co-infection in 12 and 24 h post-infection was descended by 64.3% (P<0.001) and 64.4% (P<0.05), while AIV NP was 61.5% (P<0.001) and 63.0% (P<0.001), respectively. These findings indicate that co-infection of AIV and NDV is able to inhibit their replication at cellular level, laying the foundation of further study the mechanism of common infection of AIV and NDV.

CLC Number:

S852.659.5

XIE Jun, SUN Ying-jie, ZHOU Chang-luan, ZHU Shan-yuan, DING Chan, BAI Jia-lin. The Effect of Co-infection of H9N2 Subtype Avian Influenza Virus and Newcastle Disease Virus on Virus Replication in the DF-1 of Chicken Embryo Fibroblasts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2521-2528.

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The Effect of Co-infection of H9N2 Subtype Avian Influenza Virus and Newcastle Disease Virus on Virus Replication in the DF-1 of Chicken Embryo Fibroblasts

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