山羊疱疹病毒Ⅰ型的分离鉴定与致病性

doi:10.11843/j.issn.0366-6964.2018.08.026

Abstract

Abstract:

This study aimed to find the cause of severe respiratory disease in fattened goat herds of many farms in Jiangsu province during 2013 to 2014. In this study, one caprine herpesvirus 1 named JSHA1405 was detected and isolated from nasal swab samples of goat herds with severe respiratory disease, and identified by transmission electron microscopy, PCR and virus pathogenicity test. MDBK cells infected with the third generation of JSHA1405 showed typical cytopathogenic effect (CPE). Under transmission electron microscopy detection, the enveloped virion could be observed with the diameter of 100 nm. Sequence analysis of gB gene indicated that JSHA1405 had some variation compared with E/CH strain, and the homology was 99.2%. Furthermore, the pathogenicity test indicated that JSHA1405 could induce high fever (peaked at 41.8℃) and lasted for about one week, other symptoms included depression, nasal discharge. Virus shedding could be detected from nasal and rectal swabs. Neutralizing antibodies appeared from the 7^th day post infection (dpi) and peaked at 28^th dpi. This is the first report about isolation, identification and pathogenicity study of CpHV-1 in China. The experiments demonstrated that the CpHV-1 strain JSHA1405 was pathogenic to goats. It provides foundation for further study of the viral etiology and the prevention and control of CpHV-1.

CLC Number:

S852.659.1

HAO Fei, MAO Li, LI Wen-liang, LI Ji-zong, YANG Lei-lei, ZHANG Wen-wen, SUN Min, ZHONG Chun-yan, LIU Mao-jun, JIANG Jie-yuan. Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1797-1802.

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References

[1] THIRY J, KEUSER V, MUYLKENS B, et al. Ruminant alphaherpesviruses related to bovine herpesvirus 1[J]. Vet Res, 2006, 37(2):169-190.
[2] DAVISON A J. Herpesvirus systematics[J]. Vet Microbiol, 2010, 143(1):52-69.
[3] BENAVIDES J, GONZÁLEZ L, DAGLEISH M, et al. Diagnostic pathology in microbial diseases of sheep or goats[J]. Vet Microbiol, 2015, 181(1-2):15-26.
[4] ROPERTO F, PRATELLI A, GUARINO G, et al. Natural caprine herpesvirus 1(CpHV-1) infection in kids[J]. J Comp Pathol, 2000, 122(4):298-302.
[5] SAITO J K, GRIBBLE D H, BERRIOS P E, et al. A new herpesvirus isolate from goats:Preliminary report[J]. Am J Vet Res, 1974, 35:847-848.
[6] METTLER F, ENGELS M, WILD P, et al. Herpesvirus infection in kids in Switzerland[J]. Schweiz Arch Tierheilkd, 1979, 121(12):655-662.
[7] PIPER K L, FITZGERALD C J, FICORILLI N, et al. Isolation of caprine herpesvirus 1 from a major outbreak of infectious pustular vulvovaginitis in goats[J]. Aust Vet J, 2008, 86(4):136-138.
[8] SILVA M L C R, PITUCO E M, NOGUEIRA A H C, et al. Serological evidence and risk factors associated with Caprine herpesvirus 1 in dairy goat flocks in a semiarid region of northeastern Brazil[J]. J Vet Diagn Invest, 2013, 25(1):125-128.
[9] SUAVET F, CHAMPION J L, BARTOLINI L, et al. First description of infection of caprine herpesvirus 1(CpHV-1) in goats in mainland france[J]. Pathogens, 2016, 5(1):17.
[10] 李文良, 毛立, 程素平, 等. 山羊源副流感病毒3型的分离与分子鉴定[J]. 畜牧兽医学报, 2015, 46(2):344-348.
LI W L, MAO L, CHENG S P, et al. Isolation and identification of caprine derived parainfluenza virus type 3[J]. Acta Veterinaria et Zootechnica Sinica, 2015, 46(2):344-348. (in Chinese)
[11] 郝飞, 汤德元, 李春燕, 等. 伪狂犬病病毒Guizhou-DY株的分离鉴定及致病性研究[J]. 畜牧兽医学报, 2013, 44(11):1851-1856.
HAO F, TANG D Y, LI C Y, et al. Identification and pathogenicity study of pseudorabies virus Guizhou-DY strain[J]. Acta Veterinaria et Zootechnica Sinica, 2013, 44(11):1851-1856. (in Chinese)
[12] REED L J, MUENCH H. A simple method of estimating fifty percent endpoints[J]. Am J Epidemiol, 1938, 27(3):493-497.
[13] CHAKRABORTY S, KUMAR A, TIWARI R, et al. Advances in diagnosis of respiratory diseases of small ruminants[J]. Vet Med Int, 2014, 2014:508304.
[14] BANYARD A, WANG Z L, PARIDA S. Peste des petits ruminants virus, eastern Asia[J]. Emerg Infect Dis, 2014, 20(12):2176-2178.
[15] 许健, 储岳峰, 高鹏程, 等. 我国17株绵羊肺炎支原体分子分型及其菌体蛋白免疫印迹[J]. 微生物学报, 2011, 51(10):1421-1426.
XU J, CHU Y F, GAO P C, et al. Molecular typing and immunoblotting of 17 Mycoplasma ovipneumoniae isolates from China[J]. Acta Microbiologica Sinica, 2011, 51(10):1421-1426. (in Chinese)
[16] 赵萍, 陈胜利, 郝华芳, 等. 一种山羊支原体山羊肺炎亚种多重抗原肽的小鼠免疫试验[J]. 畜牧兽医学报, 2016, 47(12):2476-2482.
ZHAO P, CHEN S L, HAO H F, et al. Immune responses in mice immunized with a recombinant multiple antigenic peptide from Mycoplasma capricolum Subsp. capripneumoniae[J]. Acta Veterinaria et Zootechnica Sinica, 2016, 47(12):2476-2482. (in Chinese)
[17] 徐春光, 郝永清, 郝瑞霞, 等. 丝状支原体山羊亚种PG3株的全基因组序列测定与分析[J]. 微生物学通报, 2017, 44(11):2669-2678.
XU C G, HAO Y Q, HAO R X, et al. Complete genome sequence and analysis of Mycoplasma mycoides subsp. capri str. PG3[J]. Microbiology China, 2017, 44(11):2669-2678. (in Chinese)
[18] DASSANAYAKE R P, SHANTHALINGAM S, SUBRAMANIAM R, et al. Role of Bibersteinia trehalosi, respiratory syncytial virus, and parainfluenza-3 virus in bighorn sheep pneumonia[J]. Vet Microbiol, 2013, 162(1):166-172.
[19] BERTOLINI S, ROSAMILIA A, CARUSO C, et al. A cross-sectional study to identify a set of risk factors for caprine herpesvirus 1 infection[J]. BMC Vet Res, 2018, 14:94.
[20] TEMPESTA M, PRATELLI A, CORRENTE M, et al. A preliminary study on the pathogenicity of a strain of caprine herpesvirus-1[J]. Comp Immunol Microbiol Infect Dis, 1999, 22(2):137-143.

Identification and Pathogenicity of One Caprine Herpesvirus 1 Strain

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