缺氧条件下巨噬细胞移动抑制因子对肉鸡心肌脂肪酸代谢的影响

doi:10.11843/j.issn.0366-6964.2018.01.022

Abstract

Abstract:

The aim of this study was to investigate the effects of macrophage migration inhibitory (MIF) on metabolism of Free-Fatty-Acid in broiler chicken cardiocytes in hypoxia. Firstly, chicken embryonic cardiomyocytes were isolated by trypsin and type Ⅱ collagenase, and cultured in DMEM, then identified by α-actinin immunocytochemistry. Secondly, hypoxia model of chicken cardiocyte induced by cobalt chloride was established, which was identified by the hypoxia sign protein, HIF-1α. Thirdly, the MIF blocker ISO-1 was used to test the relationship between MIF and p-AMPK. The expression levels of FAT/CD₃₆, p-ACC and CPT-1A in cardiomyocytes were determined by western blot during hypoxia and normoxia. Results were as follows:The chicken cardiocytes, above 90% purity, showed spherical and polygon shape in vivo, and they beat spontaneously about 80-130 times per minute. HIF-1α expression reached the highest at 24 h with the 600 μmol·L^-1 CoCl₂. The p-AMPK levels decreased significantly (P<0.01) along with the MIF which was blocked by 100 μmol·L^-1 ISO-1. The levels of MIF, p-AMPK, FAT/CD₃₆, p-ACC and CPT-1A were all increased significantly (P<0.01) in the hypoxia group compared with the normal one, while the levels of the target proteins were all decreased significantly (P<0.01) in the inhibitor group compared with the hypoxia one. MIF, a myocardial autocrine factor, plays an important protective role in the fatty acid adapted metabolism of chicken cardiomyocytes during hypoxia by activating the AMPK signal pathway.

CLC Number:

S852.2

REN Hao, LI Li-fang, WANG Yan-mei, HUANG Nan, PEI Fang-ying, LI Jia-wei, SUN Yao-gui, DUAN Zhi-bian, LI Hong-quan, WANG Wen-kui. The Effects of Macrophage Migration Inhibitory on Metabolism of Free-Fatty-Acid in Broiler Chicken Cardiocytes in Hypoxia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(1): 195-202.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2018.01.022

https://www.xmsyxb.com/EN/Y2018/V49/I1/195

References

[1] SHEN Y R, SHI S R, TONG H B, et al. Metabolomics analysis reveals that bile acids and phospholipids contribute to variable responses to low-temperature-induced ascites syndrome[J]. Mol Biosyst, 2014, 10(6):1557-1567.
[2] ZHANG J J, FENG X J, ZHAO L H, et al. Expression of hypoxia-inducible factor 1α mRNA in hearts and lungs of broiler chickens with ascites syndrome induced by excess salt in drinking water[J]. Poult Sci, 2013, 92(8):2044-2052.
[3] YANG F, CAO H B, XIAO Q Y, et al. Transcriptome analysis and gene identification in the pulmonary artery of broilers with ascites syndrome[J]. PLoS One, 2016, 11(6):e0156045.
[4] LI H Y, WANG Y M, CHEN L L, et al. The role of MIF, cyclinD1 and ERK in the development of pulmonary hypertension in broilers[J]. Avian Pathol, 2017, 46(2):202-208.
[5] TAN X, HU S H, WANG X L. Possible role of nitric oxide in the pathogenesis of pulmonary hypertension in broilers:a synopsis[J]. Avian Pathol, 2007, 36(4):261-267.
[6] LI K, ZHAO L H, GENG G R, et al. Increased calcium deposits and decreased Ca²⁺-ATPase in erythrocytes of ascitic broiler chickens[J]. Res Vet Sci, 2011, 90(3):468-473.
[7] WALLHAUS T R, TAYLOR M, DEGRADO T R, et al. Myocardial free fatty acid and glucose use after carvedilol treatment in patients with congestive heart failure[J]. Circulation, 2001, 103(20):2441-2446.
[8] RAMANNA H, ELVAN A, WITTKAMPF F H, et al. Increased dispersion and shortened refractoriness caused by verapamil in chronic atrial fibrillation[J]. J Am Coll Cardiol, 2001, 37(5):1403-1407.
[9] NEUBAUER S. The failing heart-an engine out of fuel[J]. N Engl J Med, 2007, 356(11):1140-1151.
[10] STANLEY W C, RECCHIA F A, LOPASCHUK G D. Myocardial substrate metabolism in the normal and failing heart[J]. Physiol Rev, 2005, 85(3):1093-1129.
[11] MA H, WANG J J, THOMAS D P, et al. Impaired macrophage migration inhibitory factor (MIF)-AMPK activation and ischemic recovery in the senescent heart[J]. Circulation, 2010, 122(3):282-292.
[12] MILLER E J, LI J, LENG L, et al. Macrophage migration inhibitory factor stimulates AMP-activated protein kinase in the ischaemic heart[J]. Nature, 2008, 451(7178):578-582.
[13] SIMPSON P, SAVION S. Differentiation of rat myocytes in single cell cultures with and without proliferating nonmyocardial cells. Cross-striations, ultrastructure, and chronotropic response to isoproterenol[J]. Circ Res, 1982, 50(1):101-116.
[14] WIDEMAN R F, RHOADS D D, ERF G F, et al. Pulmonary arterial hypertension (ascites syndrome) in broilers:a review[J]. Poult Sci, 2013, 92(1):64-83.
[15] OLKOWSKI A A. Pathophysiology of heart failure in broiler chickens:structural, biochemical, and molecular characteristics[J]. Poult Sci, 2007, 86(5):999-1005.
[16] HASSANZADEH M, BUYSE J, TOLOEI T, et al. Ascites syndrome in broiler chickens:a review on the aspect of endogenous and exogenous factors interactions[J]. J Poult Sci, 2014, 51(3):229-241.
[17] BAGHBANZADEH A, DECUYPERE E. Ascites syndrome in broilers:physiological and nutritional perspectives[J]. Avian Pathol, 2008, 37(2):117-126.
[18] MAXWELL P H, WIESENER M S, CHANG G W, et al. The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis[J]. Nature, 1999, 399(6733):271-275.
[19] JAAKKOLA P, MOLE D R, TIAN Y M, et al. Targeting of HIF-α to the von hippel-lindau ubiquitylation complex by O₂-regulated prolyl hydroxylation[J]. Science, 2001, 292(5516):468-472.
[20] KALLIO P J, WILSON W J, O'BRIEN S, et al. Regulation of the hypoxia-inducible transcription factor 1α by the ubiquitin-proteasome pathway[J]. J Biol Chem, 1999, 274(10):6519-6525.
[21] WELFORD S M, BEDOGNI B, GRADIN K, et al. HIF1α delays premature senescence through the activation of MIF[J]. Genes Dev, 2006, 20(24):3366-3371.
[22] RUSSELL Ⅲ R R, LI J, COVEN D L, et al. AMP-activated protein kinase mediates ischemic glucose uptake and prevents postischemic cardiac dysfunction, apoptosis, and injury[J]. J Clin Invest, 2004, 114(4):495-503.
[23] OAKHILL J S, STEEL R, CHEN Z P, et al. AMPK is a direct adenylate charge-regulated protein kinase[J]. Science, 2011, 332(6036):1433-1435.
[24] ADACHI Y, KANBAYASHI Y, HARATA I, et al. Petasin activates AMP-activated protein kinase and modulates glucose metabolism[J]. J Nat Prod, 2014, 77(6):1262-1269.
[25] WANG J Y, TONG C, YAN X Y, et al. Limiting cardiac ischemic injury by pharmacological augmentation of macrophage migration inhibitory factor-AMP-activated protein kinase signal transduction[J]. Circulation, 2013, 128(3):225-236.
[26] MU J, BROZINICK J T Jr, VALLADARES O, et al. A role for AMP-activated protein kinase in contraction-and hypoxia-regulated glucose transport in skeletal muscle[J]. Mol Cell, 2001, 7(5):1085-1094.
[27] 韩丽娟. 肉鸡腹水综合征(AS)中MIF与AMPK表达关系的研究[D]. 太谷:山西农业大学, 2015.
HAN L J. The relationship between MIF and AMPK expression in ascites syndrome (AS)[D]. Taigu:Shanxi Agricultural University, 2015. (in Chinese)
[28] PIRRO M, MAURIÈGE P, TCHERNOF A, et al. Plasma free fatty acid levels and the risk of ischemic heart disease in men:prospective results from the Québec cardiovascular study[J]. Atherosclerosis, 2002, 160(2):377-384.

The Effects of Macrophage Migration Inhibitory on Metabolism of Free-Fatty-Acid in Broiler Chicken Cardiocytes in Hypoxia

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Jie, CONG Wei, ZHAO Mindie, ZHAO Ruqian. Expression of GR and FKBP5 in Hippocampus and Hypothalamus of Arbor Acres and Rugao Yellow Broiler Chickens and Its Relationship with Stress Sensitivity [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4766-4776.
[2]	DAI Lingli, LIU Zaixia, GUO Lili, YANG Yanda, CHANG Chencheng, WANG Yu, SHI Caixia, WANG Yuzhen, ZHANG Wenguang. β-Hydroxybutyrate Mediated Epigenetic Modification and Its Molecular Mechanism of Regulating Inflammation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4095-4104.
[3]	ZHAO Huiying, YU Shiqiang, ZHAO Yuchao, JIANG Linshu. Mechanism of Liver-Adipose Tissue Crosstalk in the Development of Fatty Liver in Periparturient Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4105-4116.
[4]	CAO Xiyue, JI Xiaoxia, ZHANG Chonghao, ZHANG Yafeng, CHEN Yutao, ZHANG Yuanshu. Diminazene Aceturate Alleviates Pulmonary Fibrosis in Mice by Endogenous Activation of ACE2 to Inhibit AngⅡ-TGF-β1 Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2631-2640.
[5]	YANG Heng, LI Licai, FU Lin, XU Huihao, ZHANG Dezhi, LI Qianyong. Effect of Prostaglandin D₂ on Endocrine Function and Expression of Apoptosis-related Genes in Goat Luteal Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1652-1663.
[6]	JIN Meilin, LI Taotao, SUN Dongxiao, WEI Caihong. Research Progress of Epigenetic Regulation in Fat Deposition Mechanism of Livestock and Poultry [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 855-867.
[7]	YE Qianwen, CHEN Zhuo, LI Xin, SUN Yawei, JIN Xiaoye, LI Ziqian, WUMAIERJIANG·Yahefu, ZHONG Qi, MA Xuelian, YAO Gang. Dynamic Changes of Gut Microbiota Composition and Its Predicted Metabolism Function in One-month-old Sucking Lambs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1095-1108.
[8]	ZHANG Xiyu, ZHAI Pin, WANG Fan, DAI Yingying, ZHAO Bohao, CHEN Yang, WU Xinsheng. Expression and Function of KRT 16 in Hair Follicle Development of Angora Rabbit [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 157-167.
[9]	LIU Zhihao, JIA Peng, LAI Qi, DONG Lifeng, WU Qiujue, GAO Yanhua, TIAN Zhonghong, DIAO Qiyu. Characteristics of Rumen Fermentation and Methane Production in Different Parity Dry Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4296-4305.
[10]	CAO Xiyue, FU Yali, XIE Nana, ZHANG Yafeng, ZHANG Yuanshu. Inhibitory Effect and Mechanism of Endogenous Activation of Angiotensin Converting Enzyme 2 by Diminazene Aceturate on Cell Lipid Deposition [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2729-2738.
[11]	GUO Wenliang, XU Yuanqing, JIN Xiao, SHI Binlin. Moderating Role of Heat Shock Protein Under Inflammatory Response and Oxidative Stress Caused by Cold Stress [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1668-1677.
[12]	YUAN Jing, ZHANG Xinxin, ZHOU Di, YANG Shuai, ZHOU Jiayan, LIANG Aixin, YANG Liguo. Evaluation of the Effect of SS and CST Dual-expression DNA Vaccines with Attenuated Salmonella as Carrier in Immunizing Calves through Different Routes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1914-1924.
[13]	SUN Yanjin, XUE Ya’nan, ZHONG Tao, WANG Linjie, LI Li, ZHANG Hongping, ZHAN Siyuan. The Function of HuR and Its Regulation on Muscle Growth and Development [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1345-1353.
[14]	DING Jun, FU Zilin, HE Junhao, DUAN Xiaowei, MA Lu, BU Dengpan. Research Progress of Milk-derived Exosomes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1019-1029.
[15]	LI Yaqi, WANG Xianzhong, ZHANG Jiaojiao. Research Progress of Cell Proliferation and Apoptosis Regulated by Non-thermal Plasma and Its Application in Animal Husbandry [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 1-10.