不同致病性H9N2亚型禽流感病毒诱导鸡TLR-7和Mx基因mRNA转录水平的差异

doi:10.11843/j.issn.0366-6964.2017.05.015

Abstract

Abstract:

For a better understanding of the mechanism underlying the expression of type Ⅰ IFN induced by H9N2 influenza viruses with different pathogenicity, mRNA of TLR-7 and Mx were dynamically studied in 3 host systems of chicken which infected with strains SD/818 and SD/196 in this study. SPF chickens, SPF chicken embryos and primary chicken embryo fibroblasts were randomly divided into three groups, control group, SD/196-infected group and SD/818-infected group. The lungs, kidneys, duodenum, bursa of Fabricius, embryonic bodies and primary chicken embryo fibroblasts were collected at different time points after infection, and mRNA of TLR-7 and Mx were dynamically studied using real-time quantitative reverse transcription PCR. The results showed that transcriptional level of TLR-7 and Mx in chicken host systems induced by SD/818 were significantly higher than those induced by SD/196 except expression of TLR-7 at chicken embryos. The results suggested that expression of TLR-7 and Mx mRNA in chicken host systems were induced by H9N2 influenza virus with different pathogenicity, and transcriptional level of TLR-7 and Mx induced by SD/818 were higher than those induced by SD/196.

CLC Number:

S852.659.5

WANG Jian-lin, CAO Zhi-wei, WANG Dong-dong, YIN Yan-bo. Transcriptional Responses of TLR-7 and Mx mRNA in 3 Chicken Host Systems Infected with H9N2 Influenza Viruses with Different Pathogenicities[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 907-913.

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Transcriptional Responses of TLR-7 and Mx mRNA in 3 Chicken Host Systems Infected with H9N2 Influenza Viruses with Different Pathogenicities

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