山羊副流感病毒3型感染MDBK细胞的miRNA表达谱变化分析

doi:10.11843/j.issn.0366-6964.2017.05.014

Abstract

Abstract:

To explore the function of the cellular miRNAs in MDBK cells infected with caprine parainfluenza virus type 3 (CPIV3), we compared the miRNA expression profiles in normal MDBK cells and MDBK cells infected with 10 MOI CPIV3 for 24 hours using high-throughput sequencing. GO analysis of the host target genes and KEGG enrichment were performed. The results indicated that total 37 miRNAs were obviously differently expressed in the infected MDBK cells including 18 up-regulated miRNAs and 19 down-regulated miRNAs. The five of these miRNAs were verified by RT-qPCR, the results were well matched with those of sequencing data. GO analysis of the host target genes showed that these responded miRNAs were involved in immunoregulation, biological regulation and metabolism. KEGG enrichment analysis suggested that these genes functioned in various cellular signaling pathways, including B cell receptor signaling pathway, MAPK signaling pathway, metabolic pathways, focal adhesion, cell adhesion molecules, calcium signaling pathway. Our experiment provided the data to further explore the molecular mechanism of differential expression of cellular miRNA involved in CPIV3 pathogenesis.

CLC Number:

S852.659.5

LI Ji-zong, LI Wen-liang, MAO Li, HAO Fei, YANG Lei-lei, ZHANG Wen-wen, JIANG Jie-yuan. Identification and Analysis of the miRNA Expression Profiles in MDBK Cells in Response to the Infection of Caprine Parainfluenza Virus Type 3 (CPIV3)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 896-906.

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Identification and Analysis of the miRNA Expression Profiles in MDBK Cells in Response to the Infection of Caprine Parainfluenza Virus Type 3 (CPIV3)

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