小反刍兽疫病毒H基因DNA疫苗的构建及对小鼠的免疫原性

doi:10.11843/j.issn.0366-6964.2014.07.016

Abstract

Abstract:

The purpose of the study was to construct DNA vaccines expressing Peste des petits ruminants virus (PPRV) H protein，and to evaluate the immunogenicity in mice.The H gene of PPRV was amplified by RT-PCR，and was cloned into the eukaryotic expression vector pcDNA3.1（+）to construct recombinant plasmids pcDNA3.1-H.The H protein transiently expressed in chicken embryo fibroblast cells (CEF) was detected by indirect immunofluorescence and Western blot.Mice were inoculated with recombinant plasmids via intramuscular injection in back legs，the immunogenicity of the vaccine was evaluated by indirect ELISA，lymphocyte proliferation assay and cytokines detection.The results showed that recombinant eukaryotic expression plasmids pcDNA3.1-H was successfully constructed and could express H protein in CEF cell.High specific antibodies were induced by the vaccine.The antigen specific lymphoproliferative test stimulation index of mice immunized with the DNA vaccine was significantly enhanced compared with the control immunized group，and high level of IFN-γ and IL-4 were secreted by the immunized mice.Hence the DNA vaccine could induce humoral and cellular immune in mice and could be promising for PPR control.

CLC Number:

S852.659.5

LI Wen-chao， SUI Xiu-kun， JIN Hong-yan， LIANG Lin， WANG Wen-quan， LI Gang. Immunogenicity of DNA Vaccine Expressing H Gene of Peste des Petits Ruminants Virus in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(7): 1142-1147.

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References

［1］BANYARD A C，PARIDA S，BATTEN C，et al.Global distribution of peste des petits ruminants virus and prospects for improved diagnosis and control［J］.J Gen Virol，2010，91(12)：2885-2897.
［2］WANG Z L，BAO J Y，WU X D，et al.Peste des petits ruminants virus in Tibet，China［J］.Emerg Infect Dis，2009，15(2)：299-301.
［3］OIE.05/12/2013：Peste des petits ruminants，China (People’s Rep.of)，(Immediate notification)［EB/OL］.［2013-12-10］.http://www.oie.int/wahis_2/public/wahid.php/Reviewreport/Review?page_refer=MapFullEventReport&reportid=14492.
［4］GIBBS E P，TAYLOR W P，LAWMAN M J，et al.Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus［J］.Intervirology，1979，11(5)：268-274.
［5］CHANDRAN D，REDDY K B，VIJAYAN S P，et al.MVA recombinants expressing the fusion and hemagglutinin genes of PPRV protects goats against virulent challenge［J］.Indian J Microbiol，2010，50(3)：266-274.
［6］WANG Y，LIU G Q，CHEN Z Y，et al.Recombinant adenovirus expressing F and H fusion proteins of peste des petits ruminants virus induces both humoral and cell-mediated immune responses in goats［J］.Vet Immunol Immunopathol，2013，154(1-2)：1-7.
［7］SINNATHAMBY G，RENUKARADHYA G J，RAJASEKHAR M，et al.Immune responses in goats to recombinant hemagglutinin-neuraminidase glycoprotein of Peste des petits ruminants virus：identification of a T cell determinant［J］.Vaccine，2001，19(32)：4816-4823.
［8］CHEN W Y，HU S，QU L M，et al.A goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep［J］.Vaccine，2010，28(30)：4742-4750.
［9］DIALLO A，MINET C，LE GOFF C，et al.The threat of peste des petits ruminants：progress in vaccine development for disease control［J］.Vaccine，2007，25(30)：5591-5597.
［10］FLINGAI S，CZERWONKO M，GOODMAN J，et al.Synthetic DNA vaccines：improved vaccine potency by electroporation and co-delivered genetic adjuvants［J/OL］.Front Immunol，2013，4：Article 354.［2013-12-10］.http://journal.frontiersin.org/Journal/10.3389/fimmu.2013.00354/full.
［11］GAO H，LI K，GAO L，et al.DNA prime-protein boost vaccination enhances protective immunity against infectious bursal disease virus in chickens［J］.Vet Microbiol，2013，164(1-2)：9-17.
［12］ZHAO F，LIU S，ZHANG X，et al.CpG adjuvant enhances the mucosal immunogenicity and efficacy of a Treponema pallidum DNA vaccine in rabbits［J］.Hum Vaccin Immunother，2013，9(4)：753-760.
［13］DU J，WU X，LONG F，et al.Improvement in efficacy of DNA vaccine encoding HIV-1 Vif by LIGHT gene adjuvant［J］.Viral Immunol，2013，26(1)：68-74.

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Immunogenicity of DNA Vaccine Expressing H Gene of Peste des Petits Ruminants Virus in Mice

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