紫锥菊提取物联合柳氮磺吡啶对湿热泄泻大鼠Th17/Treg免疫失衡的影响

doi:10.11843/j.issn.0366-6964.2023.11.035

Abstract

Abstract: In this study, Echinacea purpurea extract and sulfasalazine were used to treat intestine damp-heat syndrome rats, to explore the efficacy of integrated traditional Chinese and Western Medicine on Intestine Damp-heat syndrome rats, and to provide scientific basis for the treatment of Intestine Damp-heat syndrome diseases. The rats were randomly divided into 5 groups. Except the control group, the rest of the rats were treated with "internal and external Damp-heat + Escherichia coli" to establish Damp-heat syndrome rat model. Then Echinacea purpurea extract group, sulfasalazine group and integrated traditional Chinese and Western medicine group were given 1 g·kg^-1 Echinacea purpurea extract, 0.1 g·kg^-1 sulfasalazine, 1 g·kg^-1 Echinacea purpurea extract and 0.1 g·kg^-1 sulfasalazine, respectively. The disease model was evaluated by clinical symptom score. The serum inflammatory factors and the proportion of Th17/Treg cells in PBMCs were measured. The results showed that after modeling, the rats had poor spirit, decreased dietary intake, reduced amount of exercise, rough and yellowish hair, and excreted yellowish brown soft stool. After 6 days of administration, the spirit, dietary intake and appearance of rats in Echinacea purpurea extract group, sulfasalazine group and integrated traditional Chinese and Western medicine group returned to normal. Compared with the control group, the levels of IL-17 and IL-23 in serum in the model group were significantly higher (P<0.05), and the level of TGF-β, IL-10 in serum decreased significantly (P<0.05). In PBMCs of model group, the proportion of Th17 cells increased significantly (P<0.05), the proportion of Treg cells decreased significantly (P<0.05), and the proportion of Th17/Treg increased significantly (P<0.05) compared with the control group. Compared with the model group, the levels of IL-17 and IL-23 in serum in Echinacea purpurea extract group and integrated traditional Chinese and Western medicine group were significantly lower (P<0.05), and the levels of TGF-β in serum increased significantly (P<0.05). The proportion of Th17 cells decreased significantly (P<0.05), the proportion of Treg cells increased significantly (P<0.05), and the proportion of Th17/Treg decreased significantly (P<0.05). In conclusion, Echinacea purpurea extract combined with sulfasalazine can reduce the unbalanced Th17/Treg ratio in Intestine Damp-heat syndrome model rats by regulating related cytokines, so as to alleviate Intestine Damp-heat syndrome in rats.

Key words: Echinacea purpurea extract, intestine damp-heat syndrome, Th17/Treg

CLC Number:

S853.74

CHI Xingzi, LI Yaoxing, WANG Huiting, YAN Ming’en, YANG Shijing, YANG Bowen, SUN Han, GUO Shining, SHI Dayou, WU Li, LIU Cui. Effect of Echinacea purpurea Extract Combined with Sulfasalazine on Immune Imbalance of Th17/Treg in Rats with Damp-Heat Syndrome[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4817-4826.

References 34

[1]	郭金龙,颜正华.湿阻证病理造型的实验研究[J].中医杂志,1988,29(8):59-61.GUO J L,YAN Z H.Experimental study on the pathological modeling of damp obstruction evidence[J].Journal of Traditional Chinese Medicine,1988,29(8):59-61.(in Chinese)
[2]	LI X Y,ZHANG C Y,HUI H Y,et al.Effect of Gegenqinlian decoction on intestinal mucosal flora in mice with diarrhea induced by high temperature and humidity treatment[J].3 Biotech,2021,11(2):83.
[3]	姚万玲.大肠湿热证实质及郁金散对其治疗作用机理研究[D].兰州:甘肃农业大学,2017.YAO W L.Essence of large intestined dampness-heat syndrome and treatment mechanisms of Yujin powder on it[D].Lanzhou:Gansu Agricultural University,2017.(in Chinese)
[4]	张烁,张建军,赵艺萌,等.外来植物药紫锥菊文献研究及其"中药化"理论探讨[J].中国中药杂志,2020,45(5):978-983.ZHANG S,ZHANG J J,ZHAO Y M,et al.Study on literature of Echinacea purpurea and discussion on its herbalization[J].China Journal of Chinese Materia Medica,2020,45(5):978-983.(in Chinese)
[5]	YAO W L,YANG C X,WEN Y Q,et al.Treatment effects and mechanisms of Yujin Powder on rat model of large intestine dampness-heat syndrome[J].J Ethnopharmacol,2017,202:265-280.
[6]	IACOMINO G,ROTONDI AUFIERO V,IANNACCONE N,et al.IBD:role of intestinal compartments in the mucosal immune response[J].Immunobiology,2020,225(1):151849.
[7]	LIN H H,ZHANG W Y,JIANG X P,et al.Total glucosides of paeony ameliorates TNBS-induced colitis by modulating differentiation of Th17/Treg cells and the secretion of cytokines[J].Mol Med Rep,2017,16(6):8265-8276.
[8]	阎翔,郭明阳.建立温病湿热证动物模型方法探讨[J].成都军区医院学报,2003,5(6):36-38.YAN X,GUO M Y.Experimental study of the pathological model of dampness obstruction[J].Hospital Journal of Chengdu Military Command,2003,5(6):36-38.(in Chinese)
[9]	余曼荣,贺建华,肖定福.辅助性T细胞17/调节性T细胞平衡调控炎症性肠病及其在动物生产上的应用[J].动物营养学报,2021,33(4):1925-1935.YU M R,HE J H,XIAO D F.T Helper cells 17/regulatory T cells balance regulation of inflammatory bowel disease and its application in animal production[J].Chinese Journal of Animal Nutrition,2021,33(4):1925-1935.(in Chinese)
[10]	邵美娟,严玉玺,祁青,等.中药活性成分在炎症性肠病治疗中的应用研究[J].中国中药杂志,2019,44(3):415-421.SHAO M J,YAN Y X,QI Q,et al.Application of active components from traditional Chinese medicine in treatment of inflammatory bowel disease[J].China Journal of Chinese Materia Medica,2019,44(3):415-421.(in Chinese)
[11]	邹颖.黄芩汤对TNBS诱导的结肠炎大鼠Th1/Th2和Th17/Treg细胞平衡的影响[D].广州:南方医科大学,2012:154.ZOU Y.Effect of Huangqin-Tang on the balance of Th1/Th2 and Th17/Treg cells in TNBS induced colitis in rats[D].Guangzhou:Southern Medical University,2012:154.(in Chinese)
[12]	王瑞琼,吴国泰,高慧琴,等.郁金散对抗生素相关性大肠湿热证模型大鼠Th17/Treg免疫失衡的影响[J].免疫学杂志,2018,34(12):1013-1020.WANG R Q,WU G T,GAO H Q,et al.The effect of Yujin power on TH17/Treg immune imbalance in rat model of antibiotic-related large intestine dampness-heat syndrome[J].Immunological Journal,2018,34(12):1013-1020.(in Chinese)
[13]	王超,王晓娟,朱美荣,等.蛇葡萄素对实验性结肠炎大鼠Treg/Th17细胞分化的影响[J].中国医药导报,2020,17(25):19-23.WANG C,WANG X J,ZHU M R,et al.Effect of Ampelopsin on the differentiation of Treg/Th17 cells in rats with experimental colitis[J].China Medical Herald,2020,17(25):19-23.(in Chinese)
[14]	ZHANG H C,DAI Y,LIU Y,et al.Helicobacter pylori colonization protects against chronic experimental colitis by regulating Th17/Treg balance[J].Inflamm Bowel Dis,2018,24(7):1481-1492.
[15]	宋雨鸿,贺亮,拾慧,等.黄芩汤颗粒剂治疗大肠湿热型溃疡性结肠炎疗效观察[J].吉林中医药,2013,33(2):159-160.SONG Y H,HE L,SHI H,et al.Efficacy of Huangqin-Tang granules in the treatment of ulcerative colitis with damp-heat in the large intestine[J].Jilin Journal of Traditional Chinese Medicine,2013,33(2):159-160.(in Chinese)
[16]	池幸子,杨诗靖,杨泊文,等.紫锥菊提取物对大鼠湿热泄泻的疗效及其机制[J].畜牧兽医学报,2023,54(1):380-391.CHI X Z,YANG S J,YANG B W,et al.Effect and mechanism of Echinacea purpurea extract in rats with dampness-heat diarrhea[J].Acta Veterinaria et Zootechnica Sinica,2023,54(1):380-391.(in Chinese)
[17]	黄丽.葛根芩连汤联合柳氮磺砒啶治疗湿热内蕴慢性结肠炎随机平行对照研究[J].实用中医内科杂志,2017,31(12):29-31.HUANG L.Gegen qinlian decoction combined with sulfasalazine in treatment of damp heat syndrome of chronic colitis randomized controlled study[J].Journal of Practical Traditional Chinese Internal Medicine,2017,31(12):29-31.(in Chinese)
[18]	王涛.两种不同方法建立大鼠湿热泄泻模型的比较研究[D].兰州:甘肃农业大学,2016:88.WANG T.Competitive study of DampHeat diarrhea rat model estaished with two different methods[D].Lanzhou:Gansu Agricultural University,2016:88.(in Chinese)
[19]	张亮.中兽医学大肠湿热泄泻模型的建立[J].畜牧兽医杂志,2021,40(4):133-137.ZHANG L.Establishment of large intestine damp heat diarrhea model in traditional Chinese veterinary medicine[J].Journal of Animal Science and Veterinary Medicine,2021,40(4):133-137.(in Chinese)
[20]	李梓欣,吕世洁,战瑞雪,等.紫锥菊免疫活性成分及作用机制研究进展[J].现代中药研究与实践,2021,35(6):94-97.LI Z X,LYU S J,ZHAN R X,et al.Research progress on immune active components and mechanism of echinacea[J].Research and Practice on Chinese Medicines,2021,35(6):94-97.(in Chinese)
[21]	BARNES J,ANDERSON L A,GIBBONS S,et al.Echinacea species (Echinacea angustifolia (DC.) Hell.,Echinacea pallida (Nutt.) Nutt.,Echinacea purpurea (L.) Moench):a review of their chemistry,pharmacology and clinical properties[J].J Pharm Pharmacol,2005,57(8):929-954.
[22]	莫秋芬,徐歆,毛予龙,等.紫锥菊提取物对小鼠巨噬细胞免疫功能的影响[J].中国畜牧杂志,2016,52(15):52-57.MO Q F,XU X,MAO Y L,et al.Effect of echinacea extract on immune function in murine macrophage[J].Chinese Journal of Animal Science,2016,52(15):52-57.(in Chinese)
[23]	季艳菊,钟高龙,常晓月,等.紫锥菊通过调控TLR4-NF-κB通路增强免疫抑制鸡的免疫功能[J].畜牧兽医学报,2022,53(7):2354-2363.JI Y J,ZHONG G L,CHANG X Y,et al.Echinacea enhances the immune function of immunosuppressed chicken by regulating TLR4-NF-κB pathway[J].Acta Veterinaria et Zootechnica Sinica,2022,53(7):2354-2363.(in Chinese)
[24]	顾达星,杨诗靖,石达友,等.基于网络药理学探究紫锥菊增强免疫功能的作用机制[J].黑龙江畜牧兽医,2022(9):1-5.GU D X,YANG S J,SHI D Y,et al.Study on the mechanism of Echinacea purpurea in enhancing immunity based on network pharmacology[J].Heilongjiang Animal Science and Veterinary Medicine,2022(9):1-5.(in Chinese)
[25]	闫遵祥.基于组学技术对黄白健脾口服液治疗犊牛湿热泄泻作用机制的研究[D].杨凌:西北农林科技大学,2022:145.YAN Z X.Study on the mechanism of Huangbai Jianpi decoction in treatment of dampness heat diarrhea in calves based on omics technology[D].Yangling:Northwest A&F University,2022:145(in Chinese)
[26]	谭洁.九香止泻片对湿热泄泻型肠易激综合征模型大鼠作用机制的研究[D].长沙:湖南中医药大学,2009:96.TAN J.Jiuxiang Zhixie tablets on hot and humid diarrhea-predominant irritable bowel syndrome rat model study of the mechanism[D].Changsha:Hunan University of Chinese Medicine,2009:96(in Chinese)
[27]	LI K Y,HAO Z H,DU J Y,et al.Bacteroides thetaiotaomicron relieves colon inflammation by activating aryl hydrocarbon receptor and modulating CD4+T cell homeostasis[J].Int Immunopharmacol,2021,90:107183.
[28]	ZHU X M,SHI Y Z,CHENG M,et al.Serum IL-6,IL-23 profile and Treg/Th17 peripheral cell populations in pediatric patients with inflammatory bowel disease[J].Pharmazie,2017,72(5):283-287.
[29]	黄文宇.枸杞多糖对DSS诱导的结肠炎小鼠Th1/Th2和Th17/Treg细胞平衡的影响[D].银川:宁夏医科大学,2017:67.HUANG W Y.Effect of LBP on the balance of Th1/Th2 and Th17/Treg cells in DSS induced colitis mice[D].Yinchuan:Ningxia Medical University,2017:67.(in Chinese)
[30]	张亚辉,姚万玲,文艳巧,等.郁金散对大肠湿热证大鼠血清免疫球蛋白与抗氧化相关因子的调节作用[J].畜牧兽医学报,2018,49(9):2044-2053.ZHANG Y H,YAO W L,WEN Y Q,et al.Regulation of Yujin powder on serum immunoglobulin and antioxidant related factors of large intestine dampness-heat syndrome rat[J].Acta Veterinaria et Zootechnica Sinica,2018,49(9):2044-2053.(in Chinese)
[31]	VELIKOVA T V,MITEVA L,STANILOV N,et al.Interleukin-6 compared to the other Th17/Treg related cytokines in inflammatory bowel disease and colorectal cancer[J].World J Gastroenterol,2020,26(16):1912-1925.
[32]	王立云,郭静.Th17/Treg平衡在炎症性肠病中的意义[J].国际儿科学杂志,2017,44(1):55-58.WANG L Y,GUO J.Th17/Treg balance in inflammatory bowel disease[J].International Journal of Pediatrics,2017,44(1):55-58.(in Chinese)
[33]	YAN J B,LUO M M,CHEN Z Y,et al.The function and role of the Th17/Treg cell balance in inflammatory bowel disease[J].J Immunol Res,2020,2020:8813558.
[34]	JIANG X P,HUANG X L,YANG Z P,et al.Iguratimod ameliorates inflammatory responses by modulating the Th17/Treg paradigm in dextran sulphate sodium-induced murine colitis[J].Mol Immunol,2018,93:9-19.

Effect of Echinacea purpurea Extract Combined with Sulfasalazine on Immune Imbalance of Th17/Treg in Rats with Damp-Heat Syndrome

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 34

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	CHI Xingzi, YANG Shijing, YANG Bowen, SUN Han, YAO Lili, GU Daxing, GUO Shining, SHI Dayou, WU Li, LIU Cui. Effect and Mechanism of Echinacea purpurea Extract in Rats with Dampness-heat Diarrhea [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 380-391.
[2]	ZHENG Youle, CHEN Yuhang, XIE Yue, YANG Guangyou, HE Ran, GU Xiaobin. The Effects of Recombinant Macrophage Migration Inhibitory Factor of Psoroptes ovis on the Characteristic Molecules of Th1/Th2 and Th17/Treg Type Immune Responses in Rabbits Peripheral Blood Mononuclear Cells in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 594-601.