鬼针草对感染柔嫩艾美耳球虫雏鸡肠道损伤、免疫及抗氧化功能的影响

doi:10.11843/j.issn.0366-6964.2025.11.039

Abstract

Abstract:

The aim of this study was to explore the effects of Bidens pilosa L. (B.pilosa) on cecal injury, immune function and oxidative stress in chicks infected with E. tenella. Bidens pilosa methanol extract (BP) was prepared by alcohol extraction method. A total of 200 1-day-old healthy male chicks were randomly divided into negative control (Control) group, positive control (Et) group, Chicken Coccidiosis Powder (CCP) group, 1 mL·kg^-1 BP group, 5 mL·kg^-1 BP group and 10 mL·kg^-1 BP group. At the age of 14 days, except for the negative control group, each chick in the other five groups was infected with 5×10⁴ E. tenella sporulated oocysts, and were treated with different doses of medicine. All chicks were sacrificed at the age of 21 days, and cecal tissues were taken to make pathological sections of cecum; The changes of cecal flora, intestinal barrier related gene mRNA transcription level, immune indexes and oxidative stress related factors were detected. The results showed that compared with the positive control group, 10 mL·kg^-1 BP reduced the number of cecal parasitic oocysts, reduced cecal lesions and injuries; The number of Lactobacillus in cecum was increased, the number of Clostridium and Salmonella was significantly reduced, and the number of cecal flora was restored; The mRNA transcription levels of Zona Occludens 1 (ZO>-1), Occludin, Claudin-1, Mucin-2 (MUC-2), the contents of interleukin 10 (IL-10), immunoglobulin G (IgG) and the activities of catalase (CAT), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) were significantly increased, and the contents of interleukin 2 (IL-2), interleukin 6 (IL-6) and malondialdehyde (MDA) were significantly decreased. The results indicated that 10 mL·kg^-1 BP in this test could repair the damaged divesity of cecal flora caused by E. tenella infection, improve the immune function of infected chicks, and reduce intestinal injury and oxidative stress.

Key words: Bidens pilosa, Bidens pilosa methanol extract, chicks, Eimeria tenella, intestinal injury, immune and antioxidant functions

CLC Number:

S855.9

DUAN Sizhang, LIU Yongning, SHAN Yige, LIU Qianlin, LI Yilin, AN Jian, ZHANG Jianjun. The Effect of Bidens pilosa on Intestinal Damage, Immune Function, and Antioxidant Capacity in Chicks Infected with Eimeria tenella[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(11): 5826-5838.

Figures/Tables 6

Table 1

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基因 Gene	引物序列 Primer sequences	参考序列 Reference sequence
闭锁蛋白 Occludin	正向引物(F): 5′-CGCAGATGTCCAGCGGTTACT-3′ 反向引物(R): 5′-CAGAGCAGGATGACGATGAGGAA-3′	NM_205128.1
闭合蛋白1 Claudin 1	正向引物(F): 5′-TCTTCATCATTGCAGGTCTGTC-3′ 反向引物(R): 5′-TTAACGGGTGTGAAAGGGTC-3′	NM_001013611.2
紧密连接蛋白1 ZO-1	正向引物(F): 5′-CTTCAGGTGTTTCTCTTCCTCCTC-3′ 反向引物(R): 5′-CTGTGGTTTCATGGCTGGATC-3′	XM_040680624.2
黏蛋白2 MUC-2	正向引物(F): 5′-TCAGGCTCCACAGTATCAAC-3′ 反向引物(R): 5′-GTGGTAGGACTGTGCTTGTA-3′	XM_040673077.2
β-肌动蛋白 β-actin	正向引物(F): 5′-TCCCTGGAGAAGAGCTATGA-3′ 反向引物(R): 5′-ACAGGACTCCATACCCAAGA-3′	NM_205518.2

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The Effect of Bidens pilosa on Intestinal Damage, Immune Function, and Antioxidant Capacity in Chicks Infected with Eimeria tenella

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