转录组分析揭示山羊角基皮肤的代谢适应性变化

doi:10.11843/j.issn.0366-6964.2025.10.015

Abstract

Abstract:

This study aimed to reveal the adaptive changes in sex hormone secretion patterns and the metabolic mechanisms underlying the development of horn-supporting skin structures. Six adult buck, including 3 horned and 3 polled individuals, were selected. Blood samples were collected before morning feeding, and serum sex hormone levels were measured using enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Skin samples were taken from the horn base of horned bucks and the corresponding forehead region of polled bucks for transcriptome sequencing analysis. Hormone analysis showed that the serum concentrations of progesterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were significantly lower in horned bucks than in polled bucks (P < 0.05). The testosterone-to-estradiol (T/E2) ratio was significantly higher in horned bucks (P < 0.05), but the levels of testosterone (T) and estradiol (E2) did not show significant differences. Transcriptome analysis identified 1 721 differentially expressed genes (DEGs), with 1 183 upregulated and 538 downregulated. These DEGs were significantly enriched in pathways related to metabolism (metabolic pathways), fatty acid elongation, fatty acid metabolism, extracellular matrix (ECM-receptor interaction), biosynthesis of unsaturated fatty acids, fatty acid degradation, and glycolipid metabolism. In conclusion, the findings suggest that horn-based skin requires more energy and keratin secretion for protein sheath growth, while maintaining higher structural stability by reducing cellular collagen and exoskeleton. In addition, horned bucks have reduced levels of P, FSH and LH, suggesting a shift in reproductive strategy towards horn-dependent morphological dominance and aggressive behaviour driven by high T/E2 ratios. The results of this study provide a theoretical basis for resolving the molecular regulatory mechanisms of horn growth and development in goats and the effects of horn traits on reproductive adaptations.

Key words: goat, polled trait, sex hormones, transcriptome

CLC Number:

S827.2

HUANG Yan, YANG Yuhang, LIU Tianwei, ZHU Lu, ZHANG Sihuan, LING Yinghui. Transcriptomic Analysis Reveals Metabolic Adaptive Changes in Goat Horned Skin[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 4938-4946.

Figures/Tables 7

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References 35

1	杨宇航, 杨华珍, 杨往鑫, 等. 山羊无角性状研究进展[J]. 中国畜禽种业, 2024, 20 (4): 65- 76.
	YANG Y H , YANG H Z , YANG W X , et al. Research progress on the polled trait in goats[J]. Chinese Journal of Animal Husbandry and Poultry Breeding, 2024, 20 (4): 65- 76.
2	张权威, 都萌萌, 程明, 等. 崂山奶山羊无角间性综合征生殖缺陷基因的基因型分析[J]. 中国畜牧兽医, 2022, 49 (8): 3054- 3061.
	ZHANG Q W , DU M M , CHENG M , et al. Genotypic analysis of the reproductive defect gene in polled intersex syndrome of Laoshan dairy goats[J]. Chinese Journal of Animal Science and Veterinary Medicine, 2022, 49 (8): 3054- 3061.
3	PAIHOUX E , VIGIER B , CHAFFAUX S , et al. A 11.7-kb deletion triggers intersexuality and polledness in goats[J]. Nat Genet, 2001, 29 (4): 453- 458. doi: 10.1038/ng769
4	SCHIBLER L , CRIBIU E P , OUSTRY-VAIMAN A , et al. Fine mapping suggests that the goat polled intersex syndrome and the human blepharophimosis ptosis epicanthus syndrome map to a 100-kb homologous region[J]. Genome Res, 2000, 10 (3): 311- 318. doi: 10.1101/gr.10.3.311
5	DROGEMULLER C , WOHLKE A , MOMKE S , et al. Fine mapping of the polled locus to a 1-Mb region on bovine chromosome 1q12[J]. Mamm Genome, 2005, 16 (8): 613- 620. doi: 10.1007/s00335-005-0016-0
6	DAVIS E B , BRAKORA K A , LEE A H . Evolution of ruminant headgear: a review[J]. P Roy Soc B-Biol Sci, 2011, 278 (1720): 2857- 2865.
7	BRAGULLA H H , HOMBERGER D G . Structure and functions of keratin proteins in simple, stratified, keratinized and cornified epithelia[J]. J Anat, 2009, 214 (4): 516- 559. doi: 10.1111/j.1469-7580.2009.01066.x
8	FANG X , ZHOU J , YANG Y , et al. Integrating scRNA-seq and bulk RNA-seq to explore the differentiation mechanism of human nail stem cells mediated by onychofibroblasts[J]. Front Cell Dev Biol, 2024, 12, 1416780. doi: 10.3389/fcell.2024.1416780
9	HUANG H , LIU J , AN F , et al. Retinoic acid drives surface epithelium fate determination through the TCF7-MSX2 axis[J]. Cell Mol Life Sci, 2024, 82 (1): 16. doi: 10.1007/s00018-024-05525-4
10	UBUKA T , TRUDEAU V L , PARHAR I . Editorial: Steroids and the brain[J]. Fron Endocrinol, 2020, 11, 366. doi: 10.3389/fendo.2020.00366
11	WIDELITZ R B , LIN G W , LAI Y C , et al. Morpho-regulation in diverse chicken feather formation: Integrating branching modules and sex hormone-dependent morpho-regulatory modules[J]. Dev Growth Differ, 2019, 61 (1): 124- 138. doi: 10.1111/dgd.12584
12	BOLGER A M , LOHSE M , USADEL B . Trimmomatic: a flexible trimmer for Illumina sequence data[J]. Bioinformatics, 2014, 30 (15): 2114- 2120. doi: 10.1093/bioinformatics/btu170
13	KIM D , LANGMEAD B , SALZBERG S L . HISAT: a fast spliced aligner with low memory requirements[J]. Nat Methods, 2015, 12 (4): 357- 360. doi: 10.1038/nmeth.3317
14	PERTEA M , PERTEA G M , ANTONESCU C M , et al. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads[J]. Nat Biotechnol, 2015, 33 (3): 290- 295. doi: 10.1038/nbt.3122
15	RISSO D , NGAI J , SPEED T P , et al. Normalization of RNA-seq data using factor analysis of control genes or samples[J]. Nat Biotechnol, 2014, 32 (9): 896- 902. doi: 10.1038/nbt.2931
16	YU G , WANG L G , HAN Y , et al. clusterProfiler: an R package for comparing biological themes among gene clusters[J]. OMICS, 2012, 16 (5): 284- 287. doi: 10.1089/omi.2011.0118
17	WANG Y , GUO Z Y , ZHANG C , et al. Characterization of ovarian follicles, serum steroid hormone concentration, and steroidogenic gene expression profiles in the developing ovarian follicles in White King pigeons[J]. J Poult Sci, 2023, 102 (7): 102673. doi: 10.1016/j.psj.2023.102673
18	KETCHEM J M , BOWMAN E J , ISALES C M . Male sex hormones, aging, and inflammation[J]. Biogerontology, 2023, 24 (1): 1- 25. doi: 10.1007/s10522-022-10002-1
19	ABUMADIGHEM A , COHEN O , HULEIHEL M . Elucidating the transcriptional states of spermatogenesis-joint analysis of germline and supporting cell, mice and human, normal and perturbed, bulk and single-cell RNA-Seq[J]. Biomolecules, 2024, 14 (7): 840. doi: 10.3390/biom14070840
20	MILLER J M , FESTA-BIANCHET M , COLTMAN D W . Genomic analysis of morphometric traits in bighorn sheep using the Ovine Infinium^® HD SNP BeadChip[J]. Peer J, 2018, 6, e4364. doi: 10.7717/peerj.4364
21	SIRASANAGANDLA S R , AI-HUSEINI I , SAKR H , et al. Natural products in mitigation of bisphenol a toxicity: Future therapeutic use[J]. Molecules, 2022, 27 (17): 5384. doi: 10.3390/molecules27175384
22	CHEN H , ZHANG J , YAN Y , et al. N6-methyladenosine RNA demethylase ALKBH5 is testis-specifically downregulated in hybrid male sterile dzo and is a target gene of bta-miR-200a[J]. Theriogenology, 2022, 187, 51- 57. doi: 10.1016/j.theriogenology.2022.04.022
23	HA J , SHIN J , SEOK E , et al. Estradiol and progesterone regulate NUCB2/nesfatin-1 expression and function in GH3 pituitary cells and THESC endometrial cells[J]. Anim Cells Sys, 2023, 27 (1): 129- 137. doi: 10.1080/19768354.2023.2226735
24	PATANI A , BALRAMD , YADAV V K , et al. Harnessing the power of nutritional antioxidants against adrenal hormone imbalance-associated oxidative stress[J]. Front Endocrinol, 2023, 14, 1271521. doi: 10.3389/fendo.2023.1271521
25	MBIYDZENYUY N E , QULU L A . Stress, hypothalamic-pituitary-adrenal axis, hypothalamic-pituitary-gonadal axis, and aggression[J]. Metab Brain Dis, 2024, 39 (8): 1613- 1636. doi: 10.1007/s11011-024-01393-w
26	FUCHS E . Scratching the surface of skin development[J]. Nature, 2007, 445 (7130): 834- 842. doi: 10.1038/nature05659
27	SCHWEIZER J , BOWDEN P E , COULOMBE P A , et al. New consensus nomenclature for mammalian keratins[J]. J Cell Biol, 2006, 174 (2): 169- 174. doi: 10.1083/jcb.200603161
28	DEY P , RAJALAXMI S , SAHA P , et al. Cold-shock proteome of myoblasts reveals role of RBM3 in promotion of mitochondrial metabolism and myoblast differentiation[J]. Commun Biol, 2024, 7 (1): 515. doi: 10.1038/s42003-024-06196-4
29	ZHANG T , NIU Q , WANG T , et al. Comparative transcriptomic analysis reveals diverse expression pattern underlying fatty acid composition among different beef cuts[J]. Foods, 2022, 11 (1): 117. doi: 10.3390/foods11010117
30	DE A , POEHLAND R . Lipid metabolism in bovine oocytes and early embryos under in vivo, in vitro, and stress conditions[J]. Int J Mol Sci, 2021, 22 (7): 3421. doi: 10.3390/ijms22073421
31	SOFⅡ I , FAUZI A R . Comparing the effect of tissue adhesive and suturing material on collagen Ⅰ/Ⅲ ratio in abdominal skin wounds: an experimental study[J]. Ann Med Surg, 2023, 85 (11): 5450- 5453. doi: 10.1097/MS9.0000000000001206
32	ANDERSEN C , UVEBRANT K , MORI Y , et al. Human integrin α10β1-selected mesenchymal stem cells home to cartilage defects in the rabbit knee and assume a chondrocyte-like phenotype[J]. Stem Cell Res Ther, 2022, 13 (1): 206. doi: 10.1186/s13287-022-02884-2
33	YONG B , FEI X , SHAO H , et al. Recombinant expression and biochemical characterization of a novel keratinase BsKER71 from feather degrading bacterium Bacillus subtilis S1-4[J]. AMB Express, 2020, 10 (1): 9. doi: 10.1186/s13568-019-0939-6
34	KWON H M , PARK J H , CHUNG K W , et al. Wide phenotypic spectrum of PNMHH patients with p. R941L mutation in MYH14[J]. J Clin Neurol, 2022, 18 (2): 238- 240. doi: 10.3988/jcn.2022.18.2.238
35	LEAL-GUTIERREZ J D , ELZO M A , CARR C , et al. RNA-seq analysis identifies cytoskeletal structural genes and pathways for meat quality in beef[J]. PLoS One, 2020, 15 (11): e0240895. doi: 10.1371/journal.pone.0240895

基因 Gene	上游引物(5′→3′) Forward primer	下游引物(5′→3′) Reverse primer
ARPC5L	GAATCAAGCCGTGAAGGAGC	ACAGCCCTGCTCGATTTCACT
ACADM	AACCAGACCACCAGTTGCAG	CATTGCCATTTCAGCCAGCA
MYH14	GCCCTGGAAGCCGACCAT	CGAACAGGAACGGCTGGG
ITGA7	CAGGGACTGTGGGATGGGATT	CTTGAACTGCTGCCGGTCTT
ITGA5	GGGAACCTCACCTACGGCTA	GTAGGAGGCCATCTGTTCCC
GAPDH	CTTCAACAGCGACACTCACTCT	CCACCACCCTGTTGCTGTA

表达变化 Change	基因 Gene	变化倍数的对数 log₂(fold change)	P-value	FDR
上调 Up-regulated	ACADM	2.11	8.51×10^-5	3.17×10^-3
	ACADSB	1.96	5.31×10^-4	1.07×10^-2
	ACOX3	1.29	5.61×10^-5	2.35×10^-3
	ACSL4	1.53	6.10×10^-5	2.49×10^-3
	ARPC5L	1.01	2.99×10^-3	3.23×10^-2
	CPT2	1.73	2.32×10^-4	6.10×10^-3
	ECHS1	1.37	1.56×10^-3	2.13×10^-2
	KRT42	1.35	3.65×10^-3	3.68×10^-2
	KRT83	2.16	2.44×10^-3	2.84×10^-2
	MYH14	1.08	1.92×10^-4	5.46×10^-3
下调 Down-regulated	COL1A1	-1.56	9.18×10^-5	3.33×10^-3
	COL1A2	-1.50	4.07×10^-5	1.87×10^-3
	COL6A1	-1.62	2.61×10^-4	6.58×10^-3
	COL6A2	-1.70	6.20×10^-5	2.52×10^-3
	COL6A3	-1.34	3.99×10^-3	3.90×10^-2
	COL6A6	-2.48	1.47×10^-14	3.57×10^-11
	ITGA5	-1.14	5.77×10^-3	4.91×10^-2
	ITGA7	-1.15	5.35×10^-3	4.67×10^-2
	ITGA9	-1.23	3.44×10^-4	8.05×10^-3
	ITGB5	-1.33	1.90×10^-3	2.41×10^-2
	ITGB6	-1.60	1.01×10^-3	1.62×10^-2

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Transcriptomic Analysis Reveals Metabolic Adaptive Changes in Goat Horned Skin

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