猪精子耐冻性与脂肪酸组成的研究

doi:10.11843/j.issn.0366-6964.2025.04.024

Abstract

Abstract:

In order to investigate the factors affecting the freezing tolerance of boar sperm, this study collected semen from 10 healthy Landrace boars aged 18-24 months old. The sperm motility, acrosome and membrane integrity, ATP levels, membrane fluidity, and fatty acid content of the semen in the good and poor freezing tolerance groups were detected after freezing and thawing. The results showed that the sperm total motility, progressive motility, curvilinear velocity, straight-line velocity, acrosome and membrane integrity, ATP level, and high membrane fluidity level of the thawed semen in the good freezing tolerance group were significantly higher than those in the poor freezing tolerance group (P < 0.05). The content of long-chain fatty acids such as oleic acid and linoleic acid in the sperm of the good freezing tolerance group was also significantly higher than that of the poor freezing tolerance group (P < 0.05). The sperm motility, progressive motility, linear velocity, frequency, acrosome integrity and membrane integrity, ATP level, and high membrane fluidity level in the treatment with 0.1% lipid mixture addition into semen of poor freezing tolerance group were significantly increased when compared to the those in control group (P < 0.05). In summary, this study detected boar sperm parameters before and after thawing, and found that the difference in freezing resistance of boar sperm may be due to the different content of long-chain fatty acids in sperm. Moreover, supplementing exogenous fatty acids can further improve the quality of boar post-thaw sperm, thus, it provided experimental basis for further improving the boar frozen semen production system.

Key words: boar sperm, freeze resistance ability, cryopreservation

CLC Number:

S828.3

ZHANG Hongyan, WANG Shanpeng, CAO Hailiang, MIN Lingjiang, ZHOU Kaifeng, ZHU Zhendong. Study on Freeze Resistance and Fatty Acid Composition of Boar Sperm[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(4): 1755-1767.

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参数 Parameter	GFE	PFE
活率/% TM	93.80±0.24	90.65±2.33
活力/% PM	39.64±1.42	38.30±2.67
曲线速度/(μm·s^-1 )VCL	124.90±4.62	129.45±3.77
直线速度/(μm·s^-1 )VSL	34.39±0.74^a	32.72±1.59^b
平均路径速度/(μm·s^-1 )VAP	62.83±2.51	61.14±1.98
鞭打频率/Hz BCF	39.18±2.14	40.67±1.54
头部侧向位移幅度/μm ALH	7.53±0.76	7.02±0.42
直线指数/% STR	59.98±0.81	56.07±1.92
线性指数/% LIN	30.76±0.51	28.77±0.48
振动指数/% WOB	47.69±0.64	50.30±0.71

参数 Parameter	GFE	PFE
活率/% TM	63.00±0.15^a	34.91±2.33^b
活力/% PM	29.14±2.51^a	13.90±1.94^b
曲线速度/(μm·s^-1 )VCL	104.97±8.70^a	95.45±2.89^b
直线速度/(μm·s^-1 )VSL	35.35±2.12^a	30.22±1.47^b
平均路径速度/(μm·s^-1 )VAP	52.72±3.67	46.21±1.76
鞭打频率/Hz BCF	43.19±1.82	39.06±1.11
头部侧向位移幅度/μm ALH	7.42±0.35	6.88±0.12
直线指数/% STR	62.54±0.22	63.56±0.19
线性指数/% LIN	32.40±0.64	32.53±0.36
振动指数/% WOB	49.78±0.49	49.42±0.35

脂肪酸 Fatty acid	精子活率 Sperm total motility	P值 P-value
C22:5N6	0.841^*	0.036
C22:0	0.849^*	0.032
C18:3N3	0.847^*	0.033
C18:2N6	0.837^*	0.038
C18:1N9C	0.836^*	0.038
C16:1	0.863^*	0.027

处理 Treatment	0%LM	0.01%LM	0.1%LM	1%LM
活率/% TM	38.27±1.52^b	41.28±1.17^b	51.53±1.46^a	37.16±1.93^c
活力/% PM	20.46±0.88^b	22.19±1.42^b	33.74±0.92^a	20.53±1.45^c
曲线速度/(μm·s^-1 )VCL	111.14±4.51^a	103.76±2.32^b	114.50±3.18^a	102.82±3.72^b
直线速度/(μm·s^-1 )VSL	40.16±1.73^b	39.71±2.01^b	44.32±0.87^a	36.31±1.24^c
平均路径速度/(μm·s^-1 )VAP	56.45±1.33^a	56.65±1.61^a	58.69±1.28^a	51.27±1.57^b
鞭打频率/Hz BCF	44.61±1.16^b	45.17±0.96^b	47.21±1.42^a	45.29±1.17^b
头部侧向位移幅度/μm LH	6.79±0.56^ab	6.45±0.85^b	7.36±0.15^a	6.07±0.74^b
直线指数/% STR	67.12±2.32	67.55±1.52	68.24±1.64	68.92±2.37
线性指数/% LIN	37.70±1.14^ab	38.69±0.76^a	38.15±1.21^a	34.80±0.67^b
振动指数/% WOB	52.38±1.62	50.14±1.37	51.97±1.42	49.82±1.52

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Study on Freeze Resistance and Fatty Acid Composition of Boar Sperm

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