高压均质鸡蛋卵黄对猪冷冻精子凋亡的影响

doi:10.11843/j.issn.0366-6964.2021.08.012

Abstract

Abstract: The aim of this study was to explore the effect of egg yolk (EY) treated by high pressure homogenization on the freezing effect of boar semen. Semen were collected from 5 healthy, mature Duroc boars. The ordinary egg yolk+Tris-citric acid-glucose (TCG) diluent was the control group, and the high pressure homogenized egg yolk+Tris-citric acid-glucose (TCG) diluent was the treatment group. Frozen semen samples were thawed, and the sperm motility, DNA integrity, changes in mitochondrial membrane potential, number of apoptotic cells, Caspases activity were detected. The expression of apoptosis-related genes mRNA was measured by qRT-PCR, and extracting raw data and performing data were analyzed. The results showed that, after the high pressure homogenization treatment, the vitality, motility and acrosomal integrity of pig sperms after freezing were significantly higher than those of the control group (P<0.05), which were 87.64%, 87.14% and 66.61%, respectively, higher than those of the control group for 12.58%, 5.82% and 12.48%; mitochondrial membrane potential and DNA integrity were significantly higher than those of the control group (P<0.05), which were 0.74 and 61.76%; the level of sperm apoptosis was significantly reduced (P<0.05), which was 37.74%; the Caspase-3, Caspase-8 and Caspase-9 activities of the experimental group were 17.15, 11.19 and 15.18, which were reduced by 6.17, 2.18 and 3.51 compared with the control group (P<0.05); the expression of Bax, TNF-α, and Caspase-9 genes in the control group were significantly higher than those in the treatment group (P<0.05), and the Bcl-2 gene expression was lower than that in the treatment group (P>0.05). In summary, high pressure homogenous egg yolk can improve the quality of pig sperms after freezing, promote the integrity of mitochondrial function, reduce the level of early sperm cell apoptosis and intracellular Caspase activity, and reduce the mRNA expression of apoptosis-related genes.

Key words: high pressure homogenization, egg yolk, semen cryopreservation, cell apoptosis

CLC Number:

S828.3

WANG Junyue, DAI Jianjun, ZHANG Shushan, SUN Lingwei, WU Caifeng, ZHANG Defu. Effects of High Pressure Homogeneous Egg Yolk on Apoptosis of Boar Sperm Cryopreservation[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2190-2199.

References 39

[1]	王捷,耿果霞,杨丽,等.芝麻酚对猪精液冷冻保存效果的影响[J].家畜生态学报,2018,39(6):57-61.WANG J,GENG G X,YANG L,et al.Effect of sesamol on cryopreservation of boar spermatozoa[J]. Acta Ecologae Animalis Domastici,2018,39(6):57-61.(in Chinese)
[2]	LEE S H,KIM Y J,HO KANG B,et al.Effect of nicotinic acid on the plasma membrane function and polyunsaturated fatty acids composition during cryopreservation in boar sperm[J].Reprod Domest Anim,2019,54(9):1251-1257.
[3]	JIANG Z L,LI Q W,HU J H,et al.Improvement of the quality of boar cryopreservation semen by supplementing with low density lipoprotein in diluents[J].Cryobiology,2007,54(3):301-304.
[4]	FOULKES J A.The separation of lipoproteins from egg yolk and their effect on the motility and integrity of bovine spermatozoa[J].J Reprod Fert,1977,49(2):277-284.
[5]	REN F,FENG T Y,NIU T J,et al.Notoginsenoside R1 protects boar sperm during liquid storage at 17℃.[J].Reprod Domest Anim,2020,55(9):1072-1079.
[6]	HOMAYOUNI A,SOHRABI M,AMINI M,et al.Effect of high pressure homogenization on physicochemical properties of curcumin nanoparticles prepared by antisolvent crystallization using HPMC or PVP[J].Mater Sci Eng C Mater Biol Appl,2019, 98:185-196.
[7]	CHEN X,ZHOU R Y,XU X L,et al.Structural modification by high-pressure homogenization for improved functional properties of freeze-dried myofibrillar proteins powder[J].Food Res Int,2017,100(1):193-200.
[8]	TOLEDO S Y G,WU J P.Effect of phospholipase A1 and high-pressure homogenization on the stability,toxicity,and permeability of egg Yolk/Fish oil emulsions[J].J Agric Food Chem,2020,68(34):9081-9089.
[9]	SIRVENTE H,BEAUMAL V,GAILLARD C,et al.Structuring and functionalization of dispersions containing egg yolk,plasma and granules induced by mechanical treatments[J].J Agric Food Chem,2007,55(23):9537-9544.
[10]	苏宇杰,杨新宇,周頔,等.热处理对鸡蛋蛋黄性质的影响[J].食品与发酵工业,2012,38(10):70-75.SU Y J,YANG X Y,ZHOU D,et al.Effect of heat-treatment on the properties of egg yolk[J].Food and Fermentation Industries,2012,38(10):70-75.(in Chinese)
[11]	YU C P,CHA Y,WU F,et al.Effects of limited hydrolysis and high-pressure homogenization on functional properties of oyster protein isolates[J].Molecules,2018,23(4):729.
[12]	CAO B Y,FANG L,LIU C L,et al.Effects of high hydrostatic pressure on the functional and rheological properties of the protein fraction extracted from pine nuts[J].Food Sci Technol Int,2017,24(1):53-66.
[13]	PEI Y,YANG L,WU L,et al.Combined effect of apigenin and ferulic acid on frozen-thawed boar sperm quality[J].Anim Sci J,2018,89(7):956-965.
[14]	KANIMOZHI P.PRASAD N R.Antioxidant potential of sesamol and its role on radiation-induced DNA damage in whole-body irradiated Swiss albino mice[J].Environ Toxicol Pharmacol,2009,28(2):192-197.
[15]	彭洁,易兵,戚青林.优化后精液孵育时间对精子DNA完整性、顶体反应率及IUI临床结局的影响[J].江西医药,2020,55(4):439-441.PENG J,YI B,QI Q L.Effects of optimized sperm incubation time on sperm DNA integrity,acrosome response rate and IUI clinical outcome[J].Jiangxi Medical Journal,2020,55(4):439-441.(in Chinese)
[16]	王昕.白藜芦醇对猪冷冻精子细胞凋亡及凋亡途径的影响[D].上海:上海海洋大学,2015.WANG X.Effects of resveratrol on apoptosis and apoptotic pathways of frozen boar spermatozoa[D].Shanghai:Shanghai Ocean University,2015.(in Chinese)
[17]	DEVINE M J,KITTLER J T.Mitochondria at the neuronal presynapse in health and disease[J].Nat Rev Neurosci,2018,19(2):63-80.
[18]	DE ALMEIDA T G,ALVES M B R,BATISSACO L,et al.Does low-level laser therapy on degenerated ovine testes improve post-thawed sperm characteristics?[J].Lasers Med Sci,2019,34(5):1001-1009.
[19]	TABORSKY M,SCHÜTZ D,GOFFINET O,et al.Alternative male morphs solve sperm performance/longevity trade-off in opposite directions[J].Sci Adv,2018,4(5):eaap8563.
[20]	吕松洁,付丽,范文华等.线粒体靶向抗氧化剂Mitoquinone对湖羊冻精损伤的保护作用[J].畜牧兽医学报,2019,50(12):2554-2559.LÜ S J,FU L,FAN W H,et al.Protective effect of mitochondria-targeted antioxidant mitoquinone on the damage of Hu sheep frozen sperm[J].Acta Veterinaria et Zootechnica Sinica,2019,50(12):2554-2559.(in Chinese)
[21]	HU C H,ZHUANG X J,WEI Y M,et al.Comparison of mitochondrial function in boar and bull spermatozoa throughout cryopreservation based on JC-1 staining[J].Cryo Letters,2017,38(1):75-79.
[22]	岳应权,马晓萍.冷冻保存对人精子Caspase-3表达和透明质酸酶活性的影响[J].家庭生活指南,2020(3):180.YUE Y Q,MA X P.Effect of cryopreservation on Caspase-3 expression and hyaluronidase activity in human sperm[J].Family Life Guide,2020(3):180.(in Chinese)
[23]	NAGATA S.Apoptosis and clearance of apoptotic cells[J].Annu Rev Immunol,2018,36:489-517.
[24]	李丹丹,刘蛟,王英群,等.PTD-FNK蛋白对猪精子的冷冻保护作用及其机制[J].江苏农业科学,2019,47(11):217-221.LI D D,LIU J,WANG Y Q,et al.Cryoprotective effect of PTD-FNK protein on pig sperm and its mechanism[J].Jiangsu Agricultural Sciences,2019,47(11):217-221.(in Chinese)
[25]	SONG W H,YI Y J,SUTOVSKY M,et al.Autophagy and ubiquitin-proteasome system contribute to sperm mitophagy after mammalian fertilization[J].Proc Natl Acad Sci U S A,2016,113(36):E5261-E5270.
[26]	潘赟,刘世荣,易晓华,等.抗氧化剂对山羊精液冷冻保存效果的研究进展[J].家畜生态学报,2020,41(11):6-11.PAN Y,LIU S R,YI X H,et al.Research progress of antioxidant effecton on cryopreservation of goat semen[J].Acta Ecologae Animalis Domastici,2020,41(11):6-11.(in Chinese)
[27]	TATONE C,DI EMIDIO G,BARBONETTI A,et al.Sirtuins in gamete biology and reproductive physiology:emerging roles and therapeutic potential in female and male infertility[J].Hum Reprod Update,2018,24(3):267-289.
[28]	BRUGNON F,PONS-REJRAJIH,ARTONNE C,et al.The limits for detection of activated caspases of spermatozoa by western blot in human semen[J].Andrologia,2012,44(4):265-272.
[29]	JEREMY M,GURUSUBRAMANIAN G,ROY V K.Vitamin D3 treatment regulates apoptosis,antioxidant defense system,and DNA integrity in the epididymal sperm of an aged rat model[J].Mol Reprod Dev,2019,86(12):1951-1962.
[30]	李晓斌,赵国栋,李海,等.补喂L-瓜氨酸对种公马精液品质、精清和血浆抗氧化能力的影响[J].新疆农业科学,2021,58(4):778-784.LI X B,ZHAO G D,LI H,et al.Effects of L-citrulline supplementation on semen quality,semen purity and plasma antioxidant capacity of stallion[J].Xinjiang Agricultural Sciences,2021,58(4):778-784.(in Chinese)
[31]	PROKHOROVA E A,KOPEINA G S,LAVRIK I N,et al.Apoptosis regulation by subcellular relocation of caspases[J].Sci Rep,2018,8(1):12199.
[32]	WANG Y N,HU S M,TUERDI M,et al.Initiator and executioner caspases in salivary gland apoptosis of Rhipicephalus haemaphysaloides[J].Parasit Vectors,2020,13(1):288.
[33]	XU Y R,YANG W X.Roles of three Es-Caspases during spermatogenesis and Cadmium-induced apoptosis in Eriocheir sinensis[J].Aging (Albany NY),2018,10(5):1146-1165.
[34]	XUE Y Z,SAN LUIS B,LANE D P.Intratumour heterogeneity of p53 expression;causes and consequences[J].J Pathol,2019,249(3):274-285.
[35]	FORTINI F,VIECELI D,SEGA F,et al.Estrogen receptor β-dependent Notch1 activation protects vascular endothelium against tumor necrosis factor α (TNFα)-induced apoptosis[J].J Biol Chem,2017,292(44):18178-18191.
[36]	王珺,钱芙蓉,菅芯蕊,等.绿原酸、咖啡酸苯乙酯及聚乙烯吡咯烷酮联合添加对猪冷冻精液品质的影响[J].黑龙江畜牧兽医,2021(4):43-48,153-154.WANG J,QIAN F R,GUAN X R,et al.Effects of combined addition of chlorogenic acid,phenyl ethyl caffeinate and polyvinylpyrrolidone on quality of frozen semen in pigs[J].Heilongjiang Animal Science and Veterinary Medicine, 2021(4):43-48,153-154.(in Chinese)
[37]	HAN T T,HUANG J H,GU J,et al.Hepatitis B virus surface protein induces sperm dysfunction through the activation of a Bcl2/Bax signaling cascade triggering AIF/Endo G-mediated apoptosis[J].Andrology,2020:1-12.
[38]	HAUSEMAN Z J,HARVEY E P,NEWMAN C E,et al.Homogeneous oligomers of pro-apoptotic BAX reveal structural determinants of mitochondrial membrane permeabilization[J].Mol Cell,2020,79(1):68-83.e7.
[39]	DEL OIMO E,GARCÍA-ÁLVAREZA O,MAROTO-MORALES A,et al.Estrous sheep serum enables invitro capacitation of ram spermatozoa while preventing caspase activation[J].Theriogenology,2016,85(2):351-360.

Effects of High Pressure Homogeneous Egg Yolk on Apoptosis of Boar Sperm Cryopreservation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 39

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Wanjie, CHEN Nanzhu, ZOU Huiying, ZHOU Xinyi, HAO Haisheng, PANG Yunwei, ZHU Huabin, ZHAO Xueming, YU Dawei, DU Weihua. Effects of Histone Methyltransferase ASH1L Overexpression on Proliferation and Apoptosis of Bovine Cumulus Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3358-3368.
[2]	DAN Yixin, YANG Lu, XIANG Hua, ZHANG Huanrong, REN Yupeng, YANG Falong, HE Honghong, ZHU Jiangjiang. Effects of BIRC5 on the Cycle and Apoptosis of Goat Testis Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1511-1524.
[3]	XU Songwei, XIANG Hua, ZHANG Huanrong, YANG Lu, WANG Xianjun, SALANG Wenzhu, ZHU Jiangjiang. Effects of Interfering SERBP1 on the Cell Proliferation, Cell Cycle and Apoptosis in Goat Turbinate Bone Primary Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2708-2720.
[4]	ZHANG Zhenzhen, LI Jianzeng, JIANG Ruirui, MA Yanchao, CAI Chunxia, ZHANG Lujie, GUO Yujie, JI Jinqing, HAN Lu, TIAN Yadong, KANG Xiangtao, HAN Ruili. Isolation, Culture and Identification of Chicken Chondrocytes and the Effect of Overexpression of miR-15a on Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1749-1758.
[5]	WANG Meng, YANG Chaoqun, WU Silin, TAN Jianbing, DU Xinze, LI Zhenxing, ZAN Linsen, YANG Wucai. Impact of Lycopene on Semen Cryopreservation and Fresh Semen Quality of Qinchuan Bull [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4507-4517.
[6]	WU Juye, LU Baochun, ZHU Yufan, JIA Kun. The Effects of Thrombospondin 1 on the Biological Characteristics of Canine Mammary Tumor Cells in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4470-4481.
[7]	LI Yaqi, WANG Xianzhong, ZHANG Jiaojiao. Research Progress of Cell Proliferation and Apoptosis Regulated by Non-thermal Plasma and Its Application in Animal Husbandry [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 1-10.
[8]	ZHANG Keshan, GAO Guangliang, LI Qin, ZHAO Xianzhi, LI Jing, WANG Qigui. The Function of NPFFR1 on Hormone Secretion and Apoptosis of Follicle Granulosa Cell in Geese [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2822-2831.
[9]	NIE Hai-tao, HUANG Xin-ai, LIU Hao, GUO Yi-xuan, WANG Zi-yu, ZHANG Yan-li, WAN Yong-jie, FAN Yi-xuan, ZHANG Guo-min, WANG Feng, WANG Jie. Effect of Different Nutrient Levels on Follicle Development and Glucose Metabolic Pathway Involved in Folliculogenesis of Hu Sheep during Luteal Phase [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 942-953.
[10]	YAO Yi-long, LI Yin-xia, REHEMAN Anwaier, ZHANG Jun, MENG Chun-hua, WANG Hui-li, QIAN Yong, CAO Shao-xian. Cloning of Sp1 Gene CDS Region of Hu Sheep and Its Effect on Proliferation and Apoptosis of Granulosa Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(11): 2098-2106.
[11]	FU Li-sheng, HOU Ning, WANG Xiao-yan, GAO Xue-li, LIU Chao-nan, Lü Xiao-ping, ZHENG Shi-min. Changes of Cell Apoptosis and Relevant Factor in Central Immune Organs of SPF Chicks Infected with Reticuloendotheliosis Virus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1983-1989.
[12]	GUO Shuang-shuang，LI Hui，SHI Zhen-dan，MA Wei-ming. Effects of Lipopolysaccharide on Proliferation，Apoptosis and Estradiol Secretion of in vitro Cultured Porcine Granulosa Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(4): 693-699.
[13]	FANG Jing，CUI Heng-min，CHEN Zheng-li，TANG Li. Age-related Changes in the Lymphocyte Proliferation and Apoptosis of the Tianfu Duck Thymus [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(2): 305-312.
[14]	QIU Ming-ning, HAN Cheng-quan, YANG Zhong-cai, LIU Jun, QUAN Fu-sheng, ZHANG Yong. Effect of Ovarian Stromal Cells on Co-cultured Small Antral Follicles in the Goat [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(7): 1043-1050.
[15]	WANG Lin-hui, LI Xiao-yu, JIN Li-ji, LI Shu-ying, XU Yong-ping. Efficacy of a Combination of Egg Yolk Immunoglobulins against Staphylococcus aureus Exotoxins and Capsules in the Treatment of Bovine Mastitis [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 2016-2021.