瘤胃酸中毒对山羊胃肠道功能、形态和菌群的影响

doi:10.11843/j.issn.0366-6964.2024.10.045

Abstract

Abstract:

This study utilized a model of rumen acidosis induced by the administration of corn flour to investigate its impact on the gastrointestinal tract flora structure of goats. In this experiment, eight healthy Boer goats were selected as test subjects, 4 in the control group and 4 in the acidosis group after modeling. Gastrointestinal(GI) tissues were collected for HE staining, RT-qPCR, and Western blot assay to investigate the effects of rumen acidosis on the morphology and functional proteins of goat GI tissues. Illumina MiSeq high-throughput sequencing was used to determine the 16S rRNA of the bacteria in rumen fluid and cecum content, aimed to sutdy the effect of acidosis on the bacterial communities in the gastrointestinal tracts. The results showed that, compared with the control group, the tissue morphology and structural integrity of the rumen and small intestine were damaged in the acidosis group. The relative expression levels of MCT1 mRNA and protein in rumen and duodenum were extremely significantly increased (P < 0.01), while the relative expression levels of SLC5A8 mRNA and protein were extremely significantly decreased (P < 0.01). The results of high-throughput sequencing in rumen contents showed that, the Shannon index of Alpha diversity analysis in the acidosis group was significantly lower than that in the control group (P < 0.01), and the relative richness of Bacteroidetes and Synergistetes was significantly decreased (P < 0.01). The relative abundance of Fibrobacteres and Tenericutes was significantly increased (P < 0.05), while the relative abundance of Ruminococcus and Prevotella was significantly decreased (P < 0.05). After high-throughput sequencing of cecum contents, compared with the control group, the relative richness of Ruminococcus and Oscillospira in acidosis group was significantly decreased (P < 0.05), while the relative richness of Clostridium was significantly increased (P < 0.05). In conclusion, rumen acidosis can destroy the integrity of digestive tract morphology, affect the expression of related proteins, and disturb digestive tract flora structure.

Key words: goat, rumen acidosis, gastrointestinal morphology, high-throughput sequencing, flora analysis

CLC Number:

S858.26

Daoliang ZHANG, Hongyan DING, Liuxing WANG, Wenjun TAI, Hao KONG, Chang ZHAO, Shibin FENG, Xichun WANG, Yanfeng XUE, Jinjie WU, Yu LI. Effect of Rumen Acidosis on Gastrointestinal Function, Morphology, and Microflora in Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4760-4772.

Figures/Tables 12

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References 30

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基因 Gene	基因序列号 GenBank accession no.	产物大小/bp Product size	引物序列(5′→3′) Primer sequence(5′→3′)
β-actin	NM_001314342.1	91	F：TCCTTCCTGGGCATGGAATC
			R：CGTAAAGGTCCTTGCGGATG
MCT1	XM_018046576.1	144	F：AAGAGGAGGAACTTTGAGGC
			R：TGAACACCTGGTCTGACTTC
SLC5A8	XM_018048162.1	87	F：CTATTGCCTTGTGCTTGCAT
			R：TTATAGGCTGGGATGCAGTG

项目 Parameter	参考范围 Reference range	对照组 Control group	酸中毒组 Acidosis group	P 值 P-value
pH	7.31~7.42	7.49±0.06	7.35±0.08	0.031
二氧化碳分压/mmHg PCO₂	32~46	35.50±5.20	32.50±5.07	0.442
碳酸氢根/(mmol·L^-1) HCO₃^-	20.0~29.0	24.10±1.83	16.45±4.77	0.024
阴离子间隙/(mmol·L^-1) AnGap	—	15.18±1.59	14.20±3.13	0.599
二氧化碳总量/(mmol·L^-1) TCO₂	21~28	25.08±1.84	17.33±4.89	0.025
剩余碱/(mmol·L^-1) BE	-3~3	3.10±2.14	-5.68±5.29	0.022
缓冲碱/(mmol·L^-1) BB	42~52	51.10±2.14	42.33±5.29	0.022
钠离子/(mmol·L^-1) Na⁺	144~160	143.50±5.32	140.00±3.56	0.316
钾离子/(mmol·L^-1) K⁺	3.5~5.8	4.00±0.37	3.10±0.22	0.005
氯离子/(mmol·L^-1) Cl^-	109~122	110.50±0.58	108.75±2.36	0.212

项目 Parameter	对照组 Control group	酸中毒组 Acidosis group	P 值 P-value
厚壁菌门Firmicutes	0.249 4±0.153 4	0.672 2±0.313 2	0.067
拟杆菌门Bacteroidetes	0.716 3±0.151 5	0.090 3±0.114 4	0.001
放线菌门Actinobacteria	0.001 1±0.000 2	0.186 0±0.252 2	0.193
互养菌门Synergistetes	0.004 1±0.001 4	0.000 1±0.000 1	0.001
螺旋菌门Spirochaetes	0.005 3±0.004 6	0.001 5±0.001 6	0.181
软壁菌门Tenericutes	0.000 3±0.000 4	0.000 9±0.000 6	0.021
纤维杆菌门Fibrobacteres	0.000 0±0.000 0	0.000 4±0.000 6	0.006

项目 Parameter	对照组 Control group	酸中毒组 Acidosis group	P 值 P-value
瘤胃球菌属Ruminococcus	0.008 60±0.003 41	0.000 18±0.000 15	0.003
梭菌属Clostridium	0.004 92±0.003 37	0.050 05±0.030 01	0.024
颤螺旋菌属Oscillospira	0.060 83±0.023 58	0.000 16±0.000 31	0.014
链球菌Streptococcus	0.000 00±0.000 00	0.064 83±0.064 01	0.136
双歧杆菌属Bifidobacterium	0.000 04±0.000 06	0.104 20±0.118 70	0.178
乳杆菌属Lactobacillusru	0.000 71±0.000 12	0.140 76±0.198 76	0.254
志贺杆菌Shigella	0.010 00±0.018 59	0.141 65±0.209 66	0.298
巨型球菌属Megasphaera	0.000 00±0.000 00	0.246 70±0.275 47	0.123

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Effect of Rumen Acidosis on Gastrointestinal Function, Morphology, and Microflora in Goats

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