猫传染性腹膜炎病毒N蛋白表达及其多克隆抗体制备

doi:10.11843/j.issn.0366-6964.2024.10.046

Abstract

Abstract:

This study aimed to express the nucleocapsid protein (N) of feline infectious peritonitis virus (FIPV) strain SH2021 in vitro and prepare rabbit polyclonal antibodies. Primers were designed based on the N gene sequence of FIPV strain SH2021 to construct the recombinant expression plasmid pCold-I-N. Subsequently, the recombinant plasmid was transformed into expression-competent cells BL21 (DE3), and expression was induced under conditions of 1.0 mmol·L^-1 IPTG and 16 ℃. Finally, purification was performed using a His column, and the purified recombinant protein was used to immunize New Zealand white rabbits to prepare polyclonal antibodies. The experimental results showed that the purified recombinant N protein had a size of approximately 45 ku, and the titer of the prepared rabbit polyclonal antibodies against the N protein reached 1∶512 000. The antibodies exhibited good reactivity and specificity towards the N protein. This study successfully prepared rabbit polyclonal antibodies against the FIPV N protein, which showed good reactivity and specificity, providing important tools for FIPV antigen-antibody detection and research on the biological functions of the N protein.

Key words: feline infectious peritonitis virus, feline coronavirus, N protein, gene cloning, prokaryotic expression, polyclonal antibody

CLC Number:

S852.659.6

Fukang LIU, Ligang YUAN, Da ZHANG, Aoxing TANG, Guangqing LIU, Jie ZHU. Preparation and Application of N Protein Polyclonal Antibody of Feline Infectious Peritonitis Virus SH2021 Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4773-4778.

Figures/Tables 4

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References 14

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Preparation and Application of N Protein Polyclonal Antibody of Feline Infectious Peritonitis Virus SH2021 Strain

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