羊伪结核棒状杆菌的分离鉴定及部分生物学特性分析

doi:10.11843/j.issn.0366-6964.2024.02.025

Abstract

Abstract: To clarify the infection and biological characteristics of Corynebacterium pseudotuberculosis (Cp) in surface lymph node abscesses of goats in Chongqing and Guizhou, Cp isolation and identification, pathogenicity tests, antimicrobial susceptibility tests, virulence or antibiotics resistance gene detection and phylogenetic analysis were carried out in this study. The results showed that 23 strains of Cp were isolated from 40 samples, with a separation rate of 57.5%. All the Cp isolates harbored pld, FagA, FagB, OppB, OppD, OppF, SodC, SpaC, pknG, NanH, sigE, and cp40 genes. The results of antimicrobial susceptibility tests showed that 95.65% (22/23) of Cp was sensitive to cefradine, amikacin and doxycycline, followed by penicillin, ampicillin, tetracycline, vancomycin and ciprofloxacin, with a sensitivity rate of 91.30% (21/23), while 100% of Cp isolates were resistant to furazolidone and 82.61% (19/23) resistant to neomycin and kanamycin. The detection rate of aminoglycoside O-phosphotransferase gene aph(3')-IIa and broad-spectrum class A beta-lactamase gene bla_TEM-116 were 0 and 4.35% respectively. The fusA gene of Cp was sequenced and submitted to GenBank with login numbers ON969140-ON969162, and phylogenetic analysis of fusA showed that all the Cp isolates were clustered into Cp biotype ovis. This study verified that Cp was the major pathogen responsible for surface lymph node abscesses of goats in Chongqing and Guizhou, and clarified its biological characteristics which provided information for this pathogen prevention.

Key words: Corynebacterium pseudotuberculosis, goat, virulence genes, antimicrobial susceptibility, phylogenetic analysis

CLC Number:

S852.61

SONG Yan, YUAN Yongfeng, QIAN Hongyu, LI Xincan, LUO Hongyan, WANG Zhiying, ZHOU Zuoyong. Identification and Partial Biological Characteristics Analysis of Corynebacterium pseudotuberculosis Isolated from Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 680-687.

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Identification and Partial Biological Characteristics Analysis of Corynebacterium pseudotuberculosis Isolated from Goats

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