纳米硒对犬肾衰中肾组织离子转运体表达及凋亡的影响

doi:10.11843/j.issn.0366-6964.2022.12.033

Abstract

Abstract: The purpose of this study was to investigate the effect of nano-selenium as a therapeutic drug on the expression and apoptosis of renal tissue ion transporters in dogs with acute renal failure induced by adenine. Twenty poodles weighing about 4 kg and aged about 1 year were randomly divided into blank control group (Control, fed with basal diet for 30 days) and acute renal failure model (Model, after basic health examination and adaptive feeding for two weeks, 15 days of basal diet + 15 days of feeding adenine, 75 mg·(kg·d)^-1), conventional infusion treatment group (Infusion, 15 days of feeding adenine, 75 mg·(kg·d)^-1+15 days glucose and sodium chloride injection 60 mL·(kg·d)^-1 and diuretic intravenous furosemide, 2-4 mg·kg^-1), nano-selenium treatment group (Nano-Se, 15 days of feeding adenine, 75 mg·(kg·d)^-1+15 days of feeding nano-selenium, 0.5 mg·(kg·d)^-1), nano-selenium prevention group (Prevention, 15 days of feeding nano-selenium 0.5 mg·(kg·d)^-1+15 days of feeding adenine, 75 mg·(kg·d)^-1), four dogs in each group. The expression levels of related protein genes were detected by blood biochemical analysis, urine routine analysis, immunohistochemistry of renal tissue paraffin sections, Western blot and RT-qPCR. The results show that nano-selenium treatment and prevention can effectively reduce the characteristic indicators of renal failure CRE and BUN, restore the urine specific gravity and urine pH of abnormal urine routine indicators of renal failure, and improve calcium and phosphorus metabolism in dogs with renal failure; compared with acute renal failure model, nano-selenium treatment effectively increased the mRNA and protein expression of CaSR and WNKs in renal tissue (P<0.05), and decreased the mRNA and protein expression of NKCC2 (P<0.05), thereby weakening the overcompensated reabsorption function in acute renal failure. The mRNA and protein expressions of pro-apoptotic Bax and Caspase3/9 in renal tissue of the renal failure model group were significantly down-regulated (P<0.05), which reduced the apoptosis effect of the kidney in acute renal failure.

Key words: acute renal failure in dogs, nano-selenium, ion transport, apoptosis

CLC Number:

S856.59

GAO Haihang, ZHANG Jiabin, ZHANG Mengdi, LI Hao, YANG Xiaoqi, SHI Jingjing, LIU Xin, ZHOU Donghai. Effects of Nano-selenium on the Expression and Apoptosis of Renal Tissue Ion Transporters in Dogs with Renal Failure[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4482-4489.

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Effects of Nano-selenium on the Expression and Apoptosis of Renal Tissue Ion Transporters in Dogs with Renal Failure

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