蜱携带牛丙型肝炎病毒新亚型巢式PCR检测方法的建立

doi:10.11843/j.issn.0366-6964.2022.03.030

Abstract

Abstract: A detection method is urgently needed, which not only can improve the detection and diagnostic ability for the newly discovered bovine hepacivirus strain GDZJ, BovHepV-GDZJ, but also contribute to the preliminary understanding of tick carrying BovHepV in Guangdong province. In this study, the specific primers of Nested PCR for BovHepV-GDZJ strain were designed using the gene regions with high abundance in high-throughput sequencing data as templates through macrotranscriptomics, high-throughput sequencing, and PCR. The results showed that the minimum detection value of the method for the standard plasmid containing the gene fragment of BovHepV-GDZJ strain was 6.8 copies·μL^-1, and it had no response to common pathogens carried by ticks such as arenavirus. Moreover, the positive rate was 46.08% (53/115) in the 115 tick samples collected in Zhanjiang, Guangdong. In this study, BovHepV was first detected from ticks, which revealing the possible transmission route of BovHepV. What's more, a nested PCR method for the detection of BovHepV-GDZJ strain was established to provide technical help for the detection and prevention of BovHepV in Guangdong. It also provides some reference value for the control of the HNV epidemic.

Key words: bovine hepacivirus, nested PCR, tick, high-throughput sequencin

CLC Number:

S852.653

GUO Luanying, WANG Nina, LI Hangyuan, JI Yufei, MA Jun, PEI Mingchao, SHAO Jianwei, LIU Quan. The Development of Nested PCR Assay for New Subtype of Bovine Hepacivirus in Ticks[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 972-977.

References 12

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The Development of Nested PCR Assay for New Subtype of Bovine Hepacivirus in Ticks

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