四川省松潘县牦牛体表蜱、高原鼠兔携带巴尔通体和无形体的PCR检测与进化分析

doi:10.11843/j.issn.0366-6964.2020.027

Abstract

Abstract: This study aimed to investigate Bartonella and Anaplasma infection in ticks and plateau pika in Songpan county of Sichuan province. Plateau pikas were trapped and the ticks collected from yaks were classified by morphological identification. The total DNA of ticks and Plateau pika spleens was extracted, partial sequences of 16S rRNA of ticks and Anaplasma, and rpoB gene of Bartonella were amplified by PCR, respectively. The positive products were sequenced and compared through the NCBI database. Phylogenetic trees were constructed based on 16S rRNA and rpoB for determination species of ticks, Anaplasma and Bartonella, respectively. A total of 330 ticks were collected from 3 villages and only Haemphysalis qinghaiensis was found. As for tick samples, the infection rates of Bartonella of Jinan, Shanba, and Xiabazhai were 16.7%,8.2%, and 18.3%, respectively and only B. melophagi was detected. Compared with Jinan village, the infection rate of Bartonella in Xiabazhai was higher (P<0.05); The infection rates of Anaplasma in Jinan, Shanba, and Xiabazhai were 9.8%, 12.4%, and 26.7%, respectively and only A.bovis was detected. Compared with Jinan, the infection rate of Anaplasmain Xiabazhai was higher (P<0.01); As for plateau pika, B. grahamii was detected in Jinan, Shanba, and Xiabazhai with infection rates of 8.7%, 17.9%, and 13.3%, respectively, and no significant difference observed. B. queenslandens was detected only in Xiabazhai with an infection rate of 6.7%; Bartonella sp. (MN296294) and Bartonella sp. (MN296293) were detected in Jinan and Xiabazhai, respectively. No Anaplasma spp. were detected in plateau pika and no co-infection was observed in tick and plateau pika. In Songpan, H. qinghaiensis is biological vector of Anaplasma and plateau pika being a reservoir host of Bartonella spp. B. queenslandens was detected firstly in plateau pika and there exists a risk of human infection.

Key words: Bartonella spp., Anaplasma spp., ticks, plateau pika, molecular detection, Songpan

CLC Number:

HE Xiutian, XIANG Yang, YUAN Dongbo, YANG Aiguo, FAN Xiaohu, TAN Xiong, ZHONG Yeqing, HAO Lili. PCR Detection and Phylogenetic Analysis of Bartonella spp. and Anaplasma spp. in Ixodid Ticks Collected from Yak and Plateau Pika in Songpan County of Sichuan Province[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1438-1446.

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PCR Detection and Phylogenetic Analysis of Bartonella spp. and Anaplasma spp. in Ixodid Ticks Collected from Yak and Plateau Pika in Songpan County of Sichuan Province

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