植物精油对产气荚膜梭菌感染肉仔鸡肠道损伤、肠道菌群CAZy谱和eggNOG通路的影响

doi:10.11843/j.issn.0366-6964.2023.06.023

Abstract

Abstract: The objective of this study was to investigate the effects of supplemental essential oils on gut lesions, CAZy spectrum and eggNOG pathways of intestinal flora in broilers challenged with Clostridium perfringens. A total of 112 1-day-old male Arbor Acre broiler chicks were randomly divided into two groups: Control group(Ctrl) and Essential Oil group(EO). Each group had 7 replicates and each replicate had 8 chicks. The broilers in group Ctrl were fed the basal diet, while the broilers in group EO were fed diet supplemented with 120 mg·kg^-1 of essential oils (comprised of carvacrol and thymol). All broilers were challenged with C. perfringens during d 14 to 20 and were sacrificed on d 21. The overall intestinal injury degree was observed for intestinal lesion score, and the ileal content was collected for metagenomic next-generation sequencing analysis. The results showed that the supplementation of EO significantly alleviated the gut lesions (P<0.05).The abundance of most ileal bacterial genes were increased significantly at the second level and the EC level of the carbohydrate active enzymes (CAZy) database(q<0.05) in which the proportion of glycosyltransferases was the highest. The addition of EO remarkably influenced the composition of homologous protein genomes of ileal bacteria, and the relative abundance of genes were generally reduced (q<0.05) at the second level and the orthologous group(OG) level of the eggNOG database (evolutionary genetics of genes: Non superior Orthologous Groups).In addition, most of the reduced genes were bacterial virulence related genes, such as Catalyzes_ the synthesis of GMP from XMP, DsbA and dTDP-4-dehydrorhamnose 3,5-epimerase. The above results indicated that, dietary supplementation of EO alleviated the gut lesions caused by Clostridium perfringens, which could be related to the regulation of the abundance of carbohydrate active enzymes and virulence-related genes of the intestinal flora.

Key words: necrotic enteritis, metagenome, carbohydrate active enzyme, virulence

CLC Number:

S831.5

JIANG Meihan, WEI Jintao, GUO Yuming, GUO Shuangshuang, DU Encun. Effects of Essential Oils on Gut Lesions, Carbohydrate Active Enzymes Spectrum and eggNOG Pathways of Intestinal Flora in Broilers Challenged with Clostridium perfringens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2448-2457.

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Effects of Essential Oils on Gut Lesions, Carbohydrate Active Enzymes Spectrum and eggNOG Pathways of Intestinal Flora in Broilers Challenged with Clostridium perfringens

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