胸膜肺炎放线杆菌分离株血清型的PCR鉴定和Apx毒素基因检测

doi:10.11843/j.issn.0366-6964.2023.03.044

Abstract

Abstract: The study aimed to investigate the serotypes and Apx toxin genes of Actinobacillus pleuropneumoniae clinical isolates. PCR was used to identify the serotypes and detect the Apx toxin genes of APP isolates. The results showed that 64 isolates were identified as serotypes 2, 5, 7, 8, 12, 15, 19 respectively. A total of 12 different Apx virulence factor patterns were found in 7 serotypes. The results of virulence factor analysis showed that different strains of the same serotype carried different Apx toxin genes, and the strains of different serotypes may also carry the same pattern of Apx toxin genes. A. pleuropneumoniae serotype 8 was the dominant serotype in Zhejiang Province, and serotype 19 was detected for the first time in China. There is no correlation between the Apx toxin gene and the serotype.

Key words: Actinobacillus pleuropneumoniae, serotyping, Apx, virulence factor, PCR

CLC Number:

S852.619

LU Bikai, YUAN Xiufang, XU Lihua, YU Bin, SU Fei, YE Shiyi, CHEN Yijie, JIANG Liming, ZHANG Hui, LI Junxing. Molecular Serotyping and Apx Gene Profile Analysis of Actinobacillus pleuropneumoniae Isolates[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1341-1346.

References 21

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Molecular Serotyping and Apx Gene Profile Analysis of Actinobacillus pleuropneumoniae Isolates

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