印第安纳沙门菌环介导等温扩增检测方法的建立

doi:10.11843/j.issn.0366-6964.2021.02.029

Abstract

Abstract: The objective of the present study was to establish a molecular method for rapid detection of Salmonella Indiana and overcome the shortcomings of traditional detection method rely on selective isolation, biochemical identification and serological typing. In this study, comparative genomics was used to identify the serospecific gene for S. Indiana, and a set of primers for LAMP was further designed. The specific molecular detection method for S. Indiana was established by optimizing the reaction conditions. The results showed that the method was specific and time-consuming, and the detection limit was 59 copies per reaction. The results of this method are consistent with GB4789.4—2016, and can also be used for the detection of self-coagulating bacteria. The detection method established in this study can rapid detection of S. Indiana, and will play an important role in the clinical diagnosis of this pathogen.

Key words: Salmonella Indiana, loop-mediated isothermal amplification, comparative genomics, detection

CLC Number:

S852.61

GONG Jiansen, XU Jingxiao, FU Lixia, ZHANG Di, DONG Yongyi, XU Bu, DOU Xinhong. Establishment of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Salmonella Indiana[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 560-564.

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Establishment of a Loop-Mediated Isothermal Amplification Method for Rapid Detection of Salmonella Indiana

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