鹅源副鸡禽杆菌全基因组重测序及比较基因组学分析

doi:10.11843/j.issn.0366-6964.2024.03.032

Abstract

Abstract: The aim of this paper was to analyze the differences between the genome of Avibacterium paragallinella (Apg) of goose origin and chicken. In this study, three goose Apg and four chicken Apg isolates were sequenced and their genomic components were analyzed. The results of whole gene re-sequence showed that the genome fragment size of 7 isolates was 2.567 832-2.607 127 Mb, and the GC content was between 40.80% and 40.93%. The results of SNPs homology analysis showed that three strains isolated from geese and three strains of C-AP3 from chicken were clustered on the small branch of p4chr1 strain, and the other three strains from chicken were distributed on the branches of other domestic strains. In the gene homology analysis experiment, there are 76 specific genes of goose isolate and 37 specific genes of chicken isolate. The screening results of adaptive genes in geese showed that 79 genes were co-clustered, including 22 genes encoding known proteins, and the rest encoding putative proteins. In the screening test of chicken adaptive genes, 39 genes were clustered, including 10 encoding known proteins and 29 encoding putative genes. Through comparative genomics, found 118 genes related to Apg host adaptation, which laid a foundation for further elucidation of the molecular biological mechanism of Apg cross-species infection.

Key words: Avibacterium paragallinarum, comparative genomics, whole gene resequencing, cross-species transmission

CLC Number:

S852.612

SU Wennan, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Complete Genome Re-sequence and Comparative Genomic Analysis of Avibacterium paragallinarum from Geese[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1208-1216.

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Complete Genome Re-sequence and Comparative Genomic Analysis of Avibacterium paragallinarum from Geese

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