牛体内囊胚全转录组模式解析

doi:10.11843/j.issn.0366-6964.2020.011

Abstract

Abstract: The purpose of this study was to explore the whole transcription pattern of bovine in vivo blastocysts. Three lactating cows over 15-month-old and in good health were selected, superovulation technology was used to obtain in vivo blastocysts. Single-cell whole transcriptome sequencing technology was used to detect the transcription pattern of mRNA, lncRNA, and circRNA of bovine blastocyst, and bioinformatics tools were used to analyze the related informations. As a result of the experiment, bovine blastocysts had about 19 975 mRNAs, 12 715 novel lncRNAs, and 887 circRNAs. There were 12 alternative splicing methods for mRNAs, mainly the transcription start site (TSS). GO and KEGG were used to analyze mRNAs. It was found that biological functions were mainly enriched in metabolism, cell membrane and protein binding, cell communication, cellular component organization and developmental process in GO enrichment analysis; The pathways were enriched in cell cycle, membrane transport, and chromosome tissue regulation. There were 6 types of circRNAs, of which the CLASSIC type had the largest number. This type of splicing would form a circular exon circRNA (EcircRNA). The whole transcriptome pattern of bovine in vivo blastocyst was clarified in this study, which provided new ideas for a comprehensive understanding of bovine in vivo blastocysts.

Key words: in vivo bovine blastocyst, mRNA, lncRNA, circRNA

CLC Number:

S823.3

WANG Jingjing, ZHANG Peipei, HAO Haisheng, DU Weihua, PANG Yunwei, QUAN Guobo, ZHAO Shanjiang, ZOU Huiying, ZHU Huabin, ZHAO Xueming. Analysis of Whole Transcriptome Pattern of Bovine in vivo Blastocyst[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1271-1280.

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Analysis of Whole Transcriptome Pattern of Bovine in vivo Blastocyst

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