HOTAIR与山羊皮肤黑色素沉积的关系及体外表达规律的研究

doi:10.11843/j.issn.0366-6964.2020.03.007

Abstract

Abstract: The aim of this study was to explore the relationship between goat HOX transcript antisense RNA (HOTAIR) and its partial family genes and skin pigmentation in goats as well as its expression pattern in the melanocytes. qRT-PCR was used to detect the mRNA expression level of HOTAIR, HOXD10, HOXC11 and HOXC12 genes in skin tissues of adult female Youzhou Dark-skin goats ((19.16±1.44) kg) and Banjiao goats ((23.27±3.24) kg). Then the expression pattern of these genes in mouse skin melanoma cells (B16-F10) at different proliferation and differentiation stages (1, 3, 5 and 7 d cultured) were also detected by qRT-PCR, and the relationship in expression level between HOTAIR and the other 3 genes was analyzed. The results showed that expression of HOTAIR and HOXC12 in the skin tissue of Youzhou Dark-skin goats were significantly higher than those of Banjiao goats (P<0.01), however, expression of HOXD10 in the skin tissue of Youzhou Dark-skin goats was significantly lower than that of Banjiao goats (P<0.05). Pearson correlation analysis indicated that expression level of HOTAIR was significantly positively correlated with HOXC12 (P<0.05), but significantly negatively correlated with HOXD10 (P<0.05). In the proliferation stage of B16-F10 cells, expression of HOTAIR was not significantly different (P>0.05) among different proliferation stages; The expression of HOXC11, HOXC12 and HOXD10 showed a pattern of "high-low-high", and their mRNA levels on 1 d cultured were higher than those on 3 and 5 d cultured (P<0.01); The expression level of HOTAIR was positively correlated with HOXC11 (P<0.01). At the differentiation stage of B16-F10 cells, expression of HOTAIR kept increasing during cell differentiation; Instead, expression of HOXD10 kept decreasing during this stage; Expression of HOXC11 was not significantly different (P>0.05) among different differentiation stages; Expression of HOXC12 on 1 and 5 d cultured were very significantly higher than those on 3 and 7 d cultured (P<0.01); Expression level of HOTAIR was significantly negatively correlated with HOXD10 (P<0.01). HOTAIR shows high expression level in the dark skin of goats, and its expression level remains increasing during differentiation in B16-F10 cells. HOTAIR has a negative regulatory effect on HOXD10. The interaction between HOTAIR and HOXD10 may affect skin pigmentation by regulating the differentiation of skin melanocytes in goats.

Key words: goat, HOTAIR, HOX family gene, skin, melanocyte

CLC Number:

S827.2

JIANG Jing, LI Jie, ZHOU Peng, WANG Gaofu, CHEN Cancan, FU Lin, ZHANG Li, LIU Liangjia, REN Hangxing. The Relationship between HOTAIR and Skin Pigmentation in Goats and Its Expression Pattern in vitro[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 465-474.

References 44

[1]	QUINN J J,ILIK I A,QU K,et al.Revealing long noncoding RNA architecture and functions using domain-specific chromatin isolation by RNA purification[J].Nat Biotechnol,2014,32(9):933-940.
[2]	MERCER T R,DINGER M E,MATTICK J S.Long non-coding RNAs:insights into functions[J].Nat Rev Genet,2009, 10(3):155-159.
[3]	PONTING C P,OLIVER P L,REIK W.Evolution and functions of long noncoding RNAs[J].Cell,2009,136(4):629-641.
[4]	REN H X,WANG G F,CHEN L,et al.Genome-wide analysis of long non-coding RNAs at early stage of skin pigmentation in goats (Capra hircus)[J].BMC Genomics,2016,17:67.
[5]	RINN J L,KERTESZ M,WANG J K,et al.Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs[J].Cell,2007,129(7):1311-1323.
[6]	HE S,LIU S P,ZHU H.The sequence,structure and evolutionary features of HOTAIR in mammals[J].BMC Evol Biol,2011,11:102.
[7]	ZHAO W,AN Y,LIANG Y,et al.Role of HOTAIR long noncoding RNA in metastatic progression of lung cancer[J].Eur Rev Med Pharmacol Sci,2014,18(13):1930-1936.
[8]	KIM K,JUTOORU I,CHADALAPAKA G,et al.HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer[J].Oncogene,2013,32(13):1616-1625.
[9]	KOGO R,SHIMAMURA T,MIMORI K,et al.Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers[J].Cancer Res,2011,71(20):6320-6326.
[10]	CHEN F J,SUN M,LI S Q,et al.Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis[J].Mol Carcinog,2013,52(11):908-915.
[11]	TANG L H,ZHANG W,SU B,et al.Long noncoding RNA HOTAIR is associated with motility,invasion,and metastatic potential of metastatic melanoma[J].Biomed Res Int,2013,2013:251098.
[12]	PIÉRARD G E,PIÉRARD-FRANCHIMONT C.HOX gene aberrant expression in skin melanoma:a review[J].J Skin Cancer, 2012, 2012:707260.
[13]	MAGNUSSON M,BRUN A C M,MIYAKE N,et al.HOXA10 is a critical regulator for hematopoietic stem cells and erythroid/megakaryocyte development[J].Blood,2007,109(9):3687-3696.
[14]	YAMAGUCHI Y,PASSERON T,HOASHI T,et al.Dickkopf 1(DKK1) regulates skin pigmentation and thickness by affecting Wnt/β-catenin signaling in keratinocytes[J].FASEB J,2008,22(4):1009-1020.
[15]	YAMAGUCHI Y,PASSERON T,WATABE H,et al.The effects of dickkopf 1 on gene expression and Wnt signaling by melanocytes:mechanisms underlying its suppression of melanocyte function and proliferation[J].J Invest Dermatol,2007, 127(5):1217-1225.
[16]	HARTMAN M L,TALAR B,NOMAN M Z,et al.Gene expression profiling identifies microphthalmia-associated transcription factor (MITF) and Dickkopf-1(DKK1) as regulators of microenvironment-driven alterations in melanoma phenotype[J].PLoS One,2014,9(4):e95157.
[17]	刘小辉,彭永东,周荣艳,等.坝上长尾鸡MITF基因核心启动子鉴定与分析[J].畜牧兽医学报,2018,49(6):1116-1123.LIU X H,PENG Y D,ZHOU R Y,et al.Identification and analysis of the core promoter of MITF gene in Bashang long-tail chicken[J].Acta Veterinaria et Zootechnica Sinica,2018,49(6):1116-1123.(in Chinese)
[18]	AMNDIO A R,NECSULEA A,JOYE E,et al.Hotair is dispensible for mouse development[J].PLoS Genet,2016,12(12):e1006232.
[19]	ZHOU Y,WANG C Q,LIU X,et al.Long non-coding RNA HOTAIR enhances radioresistance in MDA-MB231 breast cancer cells[J].Oncol Lett,2017,13(3):1143-1148.
[20]	CHAMCHEU J C,ROY T,UDDIN M B,et al.Role and therapeutic targeting of the PI3K/Akt/mTOR signaling pathway in skin cancer:a review of current status and future trends on natural and synthetic agents therapy[J].Cells,2019,8(8):803.
[21]	EDIRIWEERA M K,TENNEKOON K H,SAMARAKOON S R.Role of the PI3K/AKT/mTOR signaling pathway in ovarian cancer:biological and therapeutic significance[J].Semin Cancer Biol,2019,59:147-160.
[22]	HUI K K,TANAKA M.Autophagy links MTOR and GABA signaling in the brain[J].Autophagy,2019,15(10):1848-1849.
[23]	JIA J Y,ABUDU Y P,CLAUDE-TAUPIN A,et al.Galectins control MTOR and AMPK in response to lysosomal damage to induce autophagy[J].Autophagy,2019,15(1):169-171.
[24]	YANG Z B,ZENG B Y,PAN Y,et al.Autophagy participates in isoliquiritigenin-induced melanin degradation in human epidermal keratinocytes through PI3K/AKT/mTOR signaling[J].Biomed Pharmacother,2018,97:248-254.
[25]	ZHOU L,LING J J,SONG J,et al.Interleukin 10 protects primary melanocyte by activation of Stat-3 and PI3K/Akt/NF-κB signaling pathways[J].Cytokine,2016,83:275-281.
[26]	SHANG Z W,FENG H X,GUI L S,et al.Propofol promotes apoptosis and suppresses the HOTAIR-mediated mTOR/p70S6K signaling pathway in melanoma cells[J].Oncol Lett,2018,15(1):630-634.
[27]	李园,刘文艳,蔡永强,等.长链非编码RNA HOTAIR的分析及在黑色素瘤致病过程中互作分子的预测[J].畜牧兽医学报, 2017,48(8):1424-1435.LI Y,LIU W Y,CAI Y Q,et al.Analysis of long non-coding RNA HOTAIR and prediction of its interaction molecules in melanoma pathogenesis[J].Acta Veterinaria et Zootechnica Sinica,2017,48(8):1424-1435.(in Chinese)
[28]	LIU Y,LI M H,ZHANG G Q,et al.microRNA-10b overexpression promotes non-small cell lung cancer cell proliferation and invasion[J].Eur J Med Res,2013,18:41.
[29]	LI W L,LI C Y,XIONG Q,et al.microRNA-10b-5p downregulation inhibits the invasion of glioma cells via modulating homeobox B3 expression[J].Exp Ther Med,2019,17(6):4577-4585.
[30]	MA L,TERUYA-FELDSTEIN J,WEINBERG R A.Tumour invasion and metastasis initiated by microRNA-10b in breast cancer[J].Nature,2007,449(7163):682-688.
[31]	LIU Z,ZHU J M,CAO H,et al.miR-10b promotes cell invasion through RhoC-AKT signaling pathway by targeting HOXD10 in gastric cancer[J].Int J Oncol,2012,40(5):1553-1560.
[32]	卜强,汤华明,谈健,等.前列腺癌中RhoC和Rho激酶1的表达及其对丝裂原活化蛋白激酶和Akt蛋白的影响[J].中华肿瘤杂志,2011,33(3):202-206.BU Q,TANG H M,TAN J,et al.Expression of RhoC and ROCK-1 and their effects on MAPK and Akt proteins in prostate carcinoma[J].Chinese Journal of Oncology,2011,33(3):202-206.(in Chinese)
[33]	YUAN Y H,WANG H Y,LAI Y,et al.Epigenetic inactivation of HOXD10 is associated with human colon cancer via inhibiting the RHOC/AKT/MAPK signaling pathway[J].Cell Commun Signal,2019,17(1):9.
[34]	PILLAIYAR T,MANICKAM M,JUNG S H.Downregulation of melanogenesis:drug discovery and therapeutic options[J].Drug Discov Today,2017,22(2):282-298.
[35]	CHAE J,SUBEDI L,JEONG M,et al.Gomisin N inhibits melanogenesis through regulating the PI3K/Akt and MAPK/ERK signaling pathways in melanocytes[J].Int J Mol Sci,2017,18(2):E471.
[36]	WU Z Y,FU Y H,CAO J H,et al.Identification of differentially expressed miRNAs between white and black hair follicles by RNA-sequencing in the goat (Capra hircus)[J].Int J Mol Sci,2014,15(6):9531-9545.
[37]	DATAR I,KALPANA G,CHOI J,et al.Critical role of miR-10b in B-RafV600E dependent anchorage independent growth and invasion of melanoma cells[J].PLoS One,2019,14(4):e0204387.
[38]	LIANG L Y,TU Y L,LU J,et al.Dkk1 exacerbates doxorubicin-induced cardiotoxicity by inhibiting the Wnt/β-catenin signaling pathway[J].J Cell Sci,2019,132(10):jcs228478.
[39]	NIU J,LI X M,WANG X,et al.DKK1 inhibits breast cancer cell migration and invasion through suppression of β-catenin/MMP7 signaling pathway[J].Cancer Cell Int,2019,19:168.
[40]	HWANG I,PARK J H,PARK H S,et al.Neural stem cells inhibit melanin production by activation of Wnt inhibitors[J].J Dermatol Sci,2013,72(3):274-283.
[41]	TANG S C,ZHENG K,TANG Y Y,et al.Overexpression of serum exosomal HOTAIR is correlated with poor survival and poor response to chemotherapy in breast cancer patients[J].J Biosci,2019,44(2):37.
[42]	ZHOU Y,ZHANG C,QIN Q,et al.Overexpression of long non-coding RNA HOTAIR enhances breast cancer radioresistance via RhoC-Akt pathway by targeting HOXD10[J].Int J Clin Exp Pathol,2016,9(1):1-9.
[43]	BOTTI G,SCOGNAMIGLIO G,AQUINO G,et al.LncRNA HOTAIR in tumor microenvironment:what role?[J].Int J Mol Sci,2019,20(9):2279.
[44]	LI J,WANG J,ZHONG Y,et al.HOTAIR:a key regulator in gynecologic cancers[J].Cancer Cell Int,2017,17:65.

The Relationship between HOTAIR and Skin Pigmentation in Goats and Its Expression Pattern in vitro

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