刚地弓形虫钙依赖蛋白激酶9对小鼠Ana-1巨噬细胞功能的影响

doi:10.11843/j.issn.0366-6964.2017.04.017

Abstract

Abstract:

This study aimed to investigate regulation of calcium dependent protein kinase 9 of Toxoplasma gondii to the macrophages of mouse. The laser confocal microscopy was utilized to check whether CDPK9 was able to bind to Ana-1 macrophages. After co-incubation with CDPK9, the proliferation of macrophages was checked by cell counting kit-8 (CCK-8). Then apoptosis and phagocytosis of macrophages were calculated by flow cytometry. NO production of cells was evaluated by Total Nitric Oxide Assay kit and expressions of TNF-α, TGF-β1, IL-10 and IL-1β were detected by ELISA research reagent. The pictures of laser confocal microscopy showed that CDPK9 could bind to macrophages. After co-incubation with CDPK9, the proliferation of macrophages was suppressed. Moreover, both apoptosis and phagocytosis rates of cells were increased. However, NO production was up-regulated by CDPK9. Concerning cytokines, production of IL-1β and TNF-α was increased, while the production of TGF-β1 and IL-10 was decreased. Our study demonstrated that CDPK9 was able to bind to macrophages and exert its effects on the activity and functions of Ana-1 macrophages via different pathways.

CLC Number:

MA Qun-shan, LIU Xin-chao, SUN Xiao-ni, WANG Shuai, XU Li-xin, SONG Xiao-kai, YAN Ruo-feng, LI Xiang-rui. Effects of Calcium Dependent Protein Kinase 9 of Toxoplasma gondii on the Functions of Mouse Ana-1 Macrophages in vitro[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 731-739.

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Effects of Calcium Dependent Protein Kinase 9 of Toxoplasma gondii on the Functions of Mouse Ana-1 Macrophages in vitro

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