羊蜱蝇携带巴尔通体和斑点热群立克次体PCR检测及遗传关系分析

doi:10.11843/j.issn.0366-6964.2021.06.024

Abstract

Abstract: This experiment was conducted to investigate infection of Bartonella and spotted fever group rickettsiae in Melophagus ovinus in Shiqu County, Sichuan Province. M. ovinus collected from Tibetan sheep were classified by morphological identification. Total DNA of all M. ovinus were extracted and partial sequences of gltA and rpoB genes of Bartonella, and OmpA and OmpB genes of Rickettsia were amplified by PCR, respectively. The positive products were sequenced and compared through the NCBI database and phylogenetic trees were constructed based on gltA, rpoB, OmpA and OmpB for determination of Bartonella and Rickettsia, respectively. A total of 407 adult M. ovinus were collected in four villages in Shiqu County. Bartonella melophagi was detected in all four villages, with a total infection rate of 14.0% (57/407). Rickettsia raoultii was detected in Arizha, Gayi and Changxgma, with a total infection rate of 11.1% (45/407). The infection rate in Changxgma was significantly higher than that in Arizha and Gayi (P<0.05). Rickettsia sp. was only detected in Xinrong, with a total infection rate of 6.6% (8/121). No co-infection was observed in this study. In Shiqu, Tibetan sheep is the biological vector of Bartonella and Rickettsia. In this study, B. melophagi, R. raoultii and Rickettsia sp. were first detected in M. ovinus in Shiqu County.

Key words: Bartonella, SFG rickettsiae, Melophagus ovinus, Shiqu County

CLC Number:

XIANG Yang, YUAN Dongbo, HOU Wei, MO Xi, YIN Jie, YANG Aiguo, HAO Lili. PCR Detection and Genetic Relationship Analysis of Bartonella and Spotted Fever Group Rickettsiae in Melophagus ovinus[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1700-1708.

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PCR Detection and Genetic Relationship Analysis of Bartonella and Spotted Fever Group Rickettsiae in Melophagus ovinus

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[1]	HE Xiutian, XIANG Yang, YUAN Dongbo, YANG Aiguo, FAN Xiaohu, TAN Xiong, ZHONG Yeqing, HAO Lili. PCR Detection and Phylogenetic Analysis of Bartonella spp. and Anaplasma spp. in Ixodid Ticks Collected from Yak and Plateau Pika in Songpan County of Sichuan Province [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1438-1446.
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