我国部分地区羊芽囊原虫流行病学调查

doi:10.11843/j.issn.0366-6964.2021.04.021

Abstract

Abstract: Blastocystis is one of the most common protozoan in human and animal intestines. It can cause intestinal and skin diseases in human and animals. In order to understand the infection status of Blastocystis from sheep and goats in different regions, 704 fecal samples from 7 provinces and regions were investigated by molecular epidemiology based on the SSU rDNA locus, and Multilocus-sequence typing was performed for the first time on ST3 and ST4 of Blastocystis derived from sheep and goats. The results showed that, the total infection rate of Blastocystis was 13.35% (94/704), and the infection rate was significantly different among different regions and breeds. There were 5 subtypes (ST3, ST4, ST5, ST10 and ST14), among which, ST3, ST4 and ST5 were zoonotic subtypes. MLST analysis showed that ST3 was Allele 38, and there were base mutations at 2 gene loci. ST4 included Allele 42, Allele 92 and Allele 94. The results indicated that, the infection of Blastocystis was common in sheep and goats, the zoonotic subgenotype and high genetic diversity of Blastocystis were found too，so more attention should be paid on its prevention and control.

Key words: sheep, Blastocystis, SSU rDNA, subtype, allele, MLST

CLC Number:

LIU Linke, WANG Penglin, JIAN Yichen, LI Shijie, WANG Zhanming, WANG Rongjun, ZHANG Longxian, NING Changshen, JIAN Fuchun. Epidemiological Investigation of Blastocystis from Sheep and Goats in Some Areas of China[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 1061-1068.

References 32

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Epidemiological Investigation of Blastocystis from Sheep and Goats in Some Areas of China

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