鸡球虫重组蛋白rEnApiAP2对鸡免疫保护效果的观察

doi:10.11843/j.issn.0366-6964.2024.04.033

Abstract

Abstract: The recombinant protein rEnApiAP2 of Eimeria necatrix alone and in combination with the recombinant proteins rEtGAM56 or rEtGAM59 of E. tenella were used to immunize chicks. The groups immunized with rEtGAM56 or rEtGAM59 alone, and the groups unimmunized and challenged (UC) or unimmunized and unchallenged were used as controls. The chicks were infected with E. necatrix or E. tenella, respectively. The survival rate, bloody stool, weight gain (WG), relative weight gain (RWG), lesion score (LC), oocyst reduction (OR), anticoccidial index (ACI) and serum antibody level (SAL) were used as indexes to evaluate the immune protective effect of rEnApiAP2 alone and in combination with rEtGAM56 or rEtGAM59, respectively. The results showed that the survival rate of all groups was 100%, the total number of bloody stools excreted by chicks in the immunized groups was less than that in the UC group, and the WG of the immunized groups were higher than that of UC group. Among the groups immunized with rEnApiAP2 alone, the RWG, LC and ACI in the group immunized with low dosage was the highest (82.36%, 73.88% and 155.26 respectively), and the LC was the lowest (1.71). Among the groups infected with E. necatrix, the ACI in the co-immunization groups was higher than that in the groups immunized with rEnApiAP2 or rEtGAM alone, of which the ACI in the group co-immunized with rEnApiAP2 and rEtGAM56 was the highest (169.83). Among the groups infected with E. tenella, the ACI in the group immunized with rEnApiAP2 (128.37) was lower than that of the group immunized with rEtGAM56 (130.12) and the group immunized with rEtGAM59 (151.88). The ACI in the group co-immunized with rEnApiAP2 and rEtGAM59 (141.62) was close to that in the group immunized with rEtGAM59 alone, and the ACI in the group co-immunized with rEnApiAP2 and rEtGAM56 (115.46) was lower than that in the group immunized with rEtGAM56 alone. The SAL of all immunized groups was significantly higher than that of the control group (P < 0.05). In conclusion, rEnApiAP2 protein had good immunogenicity and was able to induce effective immune protection against E. necatrix, and co-immunization with rEtGAM could enhance its immune protection against E. necatrix. These results laid a foundation to develop a recombinant coccidiosis vaccine.

Key words: Eimeria necatrix, Eimeria tenella, rEnApiAP2, rEtGAM, protective efficacy

CLC Number:

S855.9

WANG Lele, WANG Liyue, CAI Weimin, KANG Xilong, FENG Qianqian, ZHANG Zhizhi, FAN Xuelian, ZHU Yu, LIU Dandan, XU Jinjun, PAN Zhiming, TAO Jianping. Immunoprotective Efficacy of Recombinant Protein rEnApiAP2 against Coccidia in Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1716-1727.

References

[1] SUO X, LI G Q. Coccidia and coccidiosis of domestic fowl[M]. Beijing:China Agricultural University Press, 1998:150-151, 173. (in Chinese)
索勋, 李国清. 鸡球虫病学[M]. 北京:中国农业大学出版社, 1998:150-151, 173.
[2] SOUTTER F, WERLING D, TOMLEY F M, et al. Poultry coccidiosis:design and interpretation of vaccine studies[J]. Front Vet Sci, 2020, 7:101.
[3] LEE Y, LU M M, LILLEHOJ H S. Coccidiosis:recent progress in host immunity and alternatives to antibiotic strategies[J]. Vaccines (Basel), 2022, 10(2):215.
[4] WALLACH M. The importance of transmission-blocking immunity in the control of infections by apicomplexan parasites[J]. Int J Parasitol, 1997, 27(10):1159-1167.
[5] LU D D, CAO L Q, ZHU Y L, et al. Cloning and characterization of an Eimeria necatrix gene encoding a gametocyte protein and associated with oocyst wall formation[J]. Parasites Vectors, 2014, 7(1):27.
[6] REID A J, BLAKE D P, ANSARI H R, et al. Genomic analysis of the causative agents of coccidiosis in domestic chickens[J]. Genome Res, 2014, 24(10):1676-1685.
[7] BALAJI S, BABU M M, IYER L M, et al. Discovery of the principal specific transcription factors of Apicomplexa and their implication for the evolution of the AP2-integrase DNA binding domains[J]. Nucleic Acids Res, 2005, 33(13):3994-4006.
[8] WANG L Y, FENG Q Q, CAI W M, et al. Cloning, expression and localization of transcription factor ApiAP2 of Eimeria necatrix[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12):4379-4388. (in Chinese)
王礼跃, 冯茜茜, 蔡为民, 等. 鸡毒害艾美耳球虫ApiAP2转录因子的克隆、表达与虫体内定位[J]. 畜牧兽医学报, 2022, 53(12):4379-4388.
[9] WANG L Y, CAI W M, ZHANG A M, et al. Protective efficacy of three recombinant gametocyte proteins of Eimeria tenella against coccidia in chick[J]. Chinese Veterinary Science, 2022, 52(6):744-752. (in Chinese)
王礼跃, 蔡为民, 张阿敏, 等. 柔嫩艾美耳球虫3种重组配子体蛋白免疫雏鸡的抗球虫免疫保护效果[J]. 中国兽医科学, 2022, 52(6):744-752.
[10] LIU Y. Cloning, expression and immunogenicity analysis of gametophyte gene Etgam56 of Eimeria tenella[D]. Yangzhou:Yangzhou University, 2021:24-25. (in Chinese)
刘悦. 柔嫩艾美耳球虫配子体基因Etgam56的克隆表达与免疫保护分析[D]. 扬州:扬州大学, 2021:24-25.
[11] ZHU Y L. Cloning and expression and its function of Etgam59 gene of Eimeria tenella[D]. Yangzhou:Yangzhou University, 2015:31-32. (in Chinese)
朱玉兰. 柔嫩艾美耳球虫Etgam59基因克隆表达与免疫保护性研究[D]. 扬州:扬州大学, 2015:31-32.
[12] MINES J J. Modifications of the McMaster worm egg counting method[J]. Aust Veterinary J, 1977, 53(7):342-343.
[13] JOHNSON J, REID W M. Anticoccidial drugs:lesion scoring techniques in battery and floor-pen experiments with chickens[J]. Exp Parasitol, 1970, 28(1):30-36.
[14] MCMANUS E C, CAMPBELL W C, CUCKLER A C. Development of resistance to quinoline coccidiostats under field and laboratory conditions[J]. J Parasitol, 1968, 54(6):1190-1193.
[15] YU S R, GUO A P. Newman Keuls Q test and Duncan's complex worst test in multiple comparisons[J]. Xinjiang Agricultural Sciences, 1981(5):15-19. (in Chinese)
俞世蓉, 郭蔼平. 多重比较时的纽曼-柯尔斯q测验和邓肯氏复极差测验[J]. 新疆农业科学, 1981(5):15-19.
[16] ZAHEER T, ABBAS R Z, IMRAN M, et al. Vaccines against chicken coccidiosis with particular reference to previous decade:progress, challenges, and opportunities[J]. Parasitol Res, 2022, 121(10):2749-2763.
[17] LI W J, CAI W M, WANG F Y, et al. Protective efficacy of co-immunization with recombinant gametocyte proteins rEnGAM22 and rEnGAM59 of Eimeria necatrix in chicken[J]. Chinese Veterinary Science, 2020, 50(12):1549-1555. (in Chinese)
李文静, 蔡为民, 汪飞燕, 等. 毒害艾美耳球虫重组配子体蛋白rEnGAM22与rEnGAM59联合免疫对鸡的保护效果观察[J]. 中国兽医科学, 2020, 50(12):1549-1555.
[18] BELLI S I, FERGUSON D J P, KATRIB M, et al. Conservation of proteins involved in oocyst wall formation in Eimeria maxima, Eimeria tenella and Eimeria acervulina[J]. Int J Parasitol, 2009, 39(10):1063-1070.
[19] SHARMAN P A, SMITH N C, WALLACH M G, et al. Chasing the golden egg:vaccination against poultry coccidiosis[J]. Parasite Immunol, 2010, 32(8):590-598.
[20] JANG S I, LILLEHOJ H S, LEE S H, et al. Eimeria maxima recombinant GAM82 gametocyte antigen vaccine protects against coccidiosis and augments humoral and cell-mediated immunity[J]. Vaccine, 2010, 28(17):2980-2985.

Immunoprotective Efficacy of Recombinant Protein rEnApiAP2 against Coccidia in Chicken

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