基于甲病毒复制子的PRRSV DNA疫苗在小鼠体内诱导的免疫应答

doi:10.11843/j.issn.0366-6964.2013.11.015

Abstract

Abstract:

We have constructed five recombinant plasmids contain modified GP3, GP5 and M genes of PRRSV based on the alphavirus replicon vector, pSCA1, named pSCA-Km5, pSCA-VPm3, pSCA-VPm5, pSCA-VP6 and pSCA-V56. And we studied the expression characteristics in vitro. Expression of proteins was confirmed by western-blotting. In order to evaluate the immunogenicity of the five plasmids in BALB/c mouse model, we detected the antibody, IL-4 and IFN-γ level by ILISA, valued the lymphocyte proliferation activity by MTS staining assay. For the group of pSCA-VPm3, pSCA-VP6, specific lymphoproliferative responses to the PRRSV stimulation were induced in the splenocytes of the immunized mice as demonstrated by MTS staining assay, and antigen specific IFN-γ was detected in the splenocytes by cytokine ELSIA. For the group of pSCA-Km5, pSCA-VPm5 and pSCA-VPm5-PEI, low-level of specific antibody was detected by ELSIA, and antigen specific IL-4 was detected in the splenocytes by cytokine ELSIA. While, the antibody and IL-4 level of the pSCA-Km5 is lower than that of the pSCA-VPm5, which indicated that VP22 gene have transduction capability. The antibody level of pSCA-VPm5-PEI was reduced after 7 weeks, which may because of its toxicity to cells. No neutralizing antibody was detected in the mice. Analysis of the data suggests that the recombined plasmids have well immunity and the capability inducing humoral and cellular immune responses in the mice, indicating that alphavirus replicon-vectored DNA-based vaccine can be potential marker vaccine against PRRS. And the statistics indicate the genes have different ability in inducing immune response.

CLC Number:

S852.659.6

LIANG Qian-qian, HOU Shao-hua, JIA Hong, YUAN Wei-feng, GUO Xiao-yu, ZHU Hong-fei. Immune Responses Induced by the DNA Vaccines Based on the Alphavirus Replicon against Porcine Reproductive and Respiratory Syndrome in Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(11): 1805-1811.

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Immune Responses Induced by the DNA Vaccines Based on the Alphavirus Replicon against Porcine Reproductive and Respiratory Syndrome in Mice

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