α-松油醇对大肠杆菌的抑菌作用及其机理研究

doi:10.11843/j.issn.0366-6964.2013.05.018

Abstract

Abstract:

The present study was undertaken to study the inhibitory effect and mechanism of α-terpineol on Escherichia coli, to provide a theoretical basis for the development of new drugs. Minimal inhibitory concentration (MIC) were determined by the same ratio dilution method with broth medium and minimal bactericidal concentration (MBC) by SDA of α-terpineol on Escherichia coli. Permeability change experiment of cell wall and membrane, nucleic acid synthesis inhibitory experiment, SDS-polyacrylamide gel electrophoresis and endotoxin inhibitory experiment, were study in the study. Results were as follows: MIC and MBC of α-terpineol on Escherichia coli are all 0.78 μL·mL^-1; Permeability of cell wall and membrane increasing; DNA fluorescence intensity is reduced and bacterial protein bands become light obviously; Endotoxin decomposed and show no typical pyrogen reaction in experimential group. It is suggested that the bacteriostatic mechanism of α-terpineol is based on its roles about changing the cell wall and membrance permeability, inhibite synthesis of nucleic acid and proteins and anti-endotoxin activity.

CLC Number:

S859.7

SHI Chao-feng, YIN Zhong-qiong,WEI Qin,JIA Ren-yong. Bacteriostatic Action and Mechanism of α-terpineol on Escherichia coli[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 796-801.

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Bacteriostatic Action and Mechanism of α-terpineol on Escherichia coli

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