1-甲基海因对慢性疼痛大鼠钠离子通道蛋白Nav1.8的干预作用

doi:10.11843/j.issn.0366-6964.2023.12.039

Abstract

Abstract: The purpose of this study is to investigate the effect of 1-methylhydantoin on Nav Channels 1.8, which can provide a theoretical basis for pain intervened and different ways to achieve the ultimate goal of "pain minimization". Sixty SPF SD rats were randomly divided into six groups.The group was treated with 1-methylhyne (group MH), lidocaine (group L) and ambroxol (group A), respectively. On the 21st and 28th day after modeling, Western blot was used to detect the protein expressions of Nav1.8 in the L4-L6 dorsal root ganglia of rats, and patch-clamp technique was used to detect the peak currents of Nav1.8 channels under the effect of MH. Comparing with the group of model,the results showed that MH could decrease the expression of Nav1.8 significantly (P<0.05). MH inhibited the channel peak currents of Nav1.8 to a certain extent. The current inhibition rates of 10 μmol·L^-1 and 100 μmol·L^-1 MH to Nav1.8 were 6.34%±3.13% and 14.6%±1.52% respectively, which is showing a dose-response relationship. It is suggested that MH can decrease the expression of Nav1.8 in DRG of rats with chronic pain, and inhibit Nav1.8 current at the same time, thus producing analgesic.

Key words: 1-methylhydantoin, rat, chronic pain, Nav1.8, patch-clamp technique

CLC Number:

S859.791

LIANG Wuying, LIU Zhen, ZENG Yuqi, Lü Junjin, MO Ruiwen, YUAN Liguo. Effect of 1-methylhydantoin on Sodium Channel Nav1.8 in Rats with Chronic Pain[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5312-5317.

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Effect of 1-methylhydantoin on Sodium Channel Nav1.8 in Rats with Chronic Pain

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