EAV-HP在SLCO1B3基因5’端非编码区整合影响芦花鸡肝脏代谢组学研究

doi:10.11843/j.issn.0366-6964.2026.01.042

Abstract

Abstract:

The formation of green eggshell characteristics in chickens is associated with the high expression of Solute Carrier Organic Anion Transporter Family Member 1B3 （SLCO1B3） in eggshell glands initiated by the endogenous retrovirus EAV-HP. As a core metabolic organ， SLCO1B3 is highly expressed in the chicken liver. The insertion mutation of EAV-HP downregulates the expression of SLCO1B3 in the chicken liver. Metabolomics studies of chicken liver during this process have not been reported. This study took the Luhua chicken as the research object. Using the green-shelled egg genotypic primers， it was determined that the frequency of green-shelled eggs produced in 38 Luhua chickens was 47.4%. Both the integration of EAV-HP^+/+ and EAV-HP^+/- significantly reduced the transcriptional level of SLCO1B3 in the liver of Luhua chicken （P<0.05）， especially the effect of the integration of EAV-HP^+/+ was especially significant. A total of 80 differential metabolites were screened out by metabolomics， among which 30 were up-regulated and 50 were down-regulated. The pathways through which metabolites are enriched mainly involve biological processes such as taurine and hypotaurine metabolism， the citric acid cycle （TCA cycle）， carbohydrate metabolism， ether lipid metabolism and alanine， aspartic acid and glutamic acid metabolism. The research results revealed the effect of down-regulation of SLCO1B3 expression caused by EAV-HP insertion mutation on liver metabolites and metabolic pathways in chickens， providing research ideas and directions for fully understanding and utilizing endogenous retroviruses to improve the production performance of chicken flocks.

Key words: EAV-HP, SLCO1B3, metabolomics, metabolites

CLC Number:

S852.2

WANG Yajun, LIU Qi, WANG Yangyang, HE Lei, WEI Ying, CHEN Songbiao, YU Zuhua, DING Ke, CHEN Jian. Study on the Effect of EAV-HP Integration in the 5’ Non-Coding Region of the SLCO1B3 Gene on the Liver Metabolomics of Luhua Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 476-485.

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References 23

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引物名称 Primer name	引物序列（5′→3′） Sequence	产物长度/bp Product size
ISU	TGTAAGAAGGCAGAGGGTTGA	500，700
ISD	GGAAACGAGGTGTGTTGTAGG
IDD	AATCTTGTCTCCTCCCAGTTC
qSLCO1B3-F	TGGTGATTGCATTTGTAAGCTA	197
qSLCO1B3-R	TGGAGAGCAGGGATTTATGC	197
qGAPDH-F	GAACATCATCCCAGCGTCCA	112
qGAPDH-R	CGGCAGGTCAGGTCAACAAC	112

芦花鸡Luhua chicken	数量/只 Number	基因型数量/只（频率/%） Number of genotypes （frequency）			绿壳蛋频率/% Green shell egg frequency	基因频率/% Gene frequency
芦花鸡Luhua chicken	数量/只 Number	N/N	LC/N	LC/LC	绿壳蛋频率/% Green shell egg frequency	N	LC
粉壳蛋 Pink shell egg	18	15（83.3）	3（16.7）	0（0）	16.7	91.65	8.35
绿壳蛋 Green shell egg	20	5（25）	13（65）	2（10）	75	57.5	42.5
总计 Total	38	20（52.6）	16（42.1）	2（5.3）	47.4	73.65	26.35

分析类型 Analysis type	分析项目 Analysis project	R²X（cum）	R²Y（cum）	Q²（cum）
PLS-DA	阳离子 Cation	0.488	0.999	0.811
PLS-DA	阴离子 Anion	0.442	0.999	0.673
OPLS-DA	阳离子 Cation	0.488	0.999	0.659
OPLS-DA	阴离子 Anion	0.442	0.999	0.552

Study on the Effect of EAV-HP Integration in the 5’ Non-Coding Region of the SLCO1B3 Gene on the Liver Metabolomics of Luhua Chicken

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