Acta Veterinaria et Zootechnica Sinica ›› 2026, Vol. 57 ›› Issue (1): 486-499.doi: 10.11843/j.issn.0366-6964.2026.01.043
• CLINICAL VETERINARY MEDICINE • Previous Articles Next Articles
MIAO Yuhang1,2(
), DING Tao1,2, XIN Jie1,2, MA Wenyan1,2, DU Jun1,2()
Received:2025-02-24
Online:2026-01-23
Published:2026-01-26
Contact:
DU Jun
E-mail:myh6943@126.com;dujun@nxu.edu.cn
CLC Number:
MIAO Yuhang, DING Tao, XIN Jie, MA Wenyan, DU Jun. Differential Expression Analysis of microRNA in Bovine Mammary Epithelial Cells Induced by Candida krusei[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 486-499.
Table 1
RT-qPCR primers for microRNA expression detection"
非编码小RNA microRNA | 引物序列（5′→3′） Primer sequence | 产物长度/bp Product length |
|---|---|---|
| Bta-miR-2377 | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGAGC 上游引物 Forward primer： GCGTGTCCACTGCACACACTA 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 21 20 |
| Bta-miR-2285i | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCCAAAA 上游引物 Forward primer： ACGAGAACAAAACCGGAACGAAC 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 23 20 |
| Bta-miR-215 | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTGTCTG 上游引物 Forward primer： GCGGCCTGATGACCTATGAATTG 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 23 20 |
| Bta-miR-362-5p | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTCAC | 50 |
| 上游引物 Forward primer： GAACTGACAATCCTTGGAACCTAGG | 25 | |
| 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 20 | |
| Bta-miR-219b-3p | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTGAT 上游引物 Forward primer： AGAAGGCAGAATTGCGTTTGGAC 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 23 20 |
| Bta-miR-146b | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACAGCC 上游引物 Forward primer： CCACCATGTGAGAACTGAATTCCAT 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 25 20 |
| Bta-miR-20b_R+3_1ss10CT | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTAC 上游引物 Forward primer： AGCTGGACCAAAGTGCTTACAGT 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 23 20 |
| Bta-miR-19b_R+2 | 茎环引物 stem-loop primer： GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACTCTC 上游引物 Forward primer： AACCTCCTAATCCTTGCTACCTGG 下游引物 Reverse primer： ATCCAGTGCAGGGTCCGAGG | 50 24 20 |
| β-Actin | 上游引物 Forward primer： AGATCAAGATCATCGCGCCC 下游引物 Reverse primer： CATTTGCGGTGGACGATGGA | 20 20 |
| U6 snRNA | 上游引物 Forward primer： CTCGCTTCGGCAGCACA 下游引物 Reverse primer： AACGCTTCACGAATTTGCGT | 17 20 |
Fig.1
Changes in the expression levels of inflammatory factors in cells"
Table 2
Quality statistics of sequencing library"
样品 Sample | 质控后序列（/%） Clean reads | 有效序列（/%） Valid reads | GC含量/% GC | Q20值/% Q20 | Q30值/% Q30 |
|---|---|---|---|---|---|
空白对照组 1 Control 1 | 8 550 695 （76.42） | 7 853 099 （91.84） | 55.21 | 94.18 | 88.93 |
空白对照组 2 Control 2 | 8 642 738 （86.55） | 8 252 043 （95.48） | 50.74 | 97.74 | 94.60 |
空白对照组3 Control 3 | 11 450 128 （88.86） | 10 954 421 （95.67） | 50.78 | 97.82 | 94.87 |
ATCC 6258感染组1 ATCC 6258 1 | 10 111 972 （90） | 9 783 952 （96.76） | 50.13 | 97.83 | 95.08 |
ATCC 6258 感染组2 ATCC 6258 2 | 10 710 185 （91.32） | 10 278 510 （95.97） | 50.35 | 97.82 | 94.80 |
ATCC 6258感染组3 ATCC 6258 3 | 10 926 138 （91.58） | 10 545 598 （96.52） | 49.97 | 97.97 | 95.29 |
Fig.2
The length distribution of identified microRNAs"
Table 3
Differential expression of microRNA between blank control group and ATCC 6258 infection group"
microRNA名称 microRNA name | 差异倍数 Log2FC | P值 P value | 上调/下调 Up/Down |
|---|---|---|---|
| mdo-miR-196a-5p_R+2 | 2.891 907 300 | 0.001 073 447 | Up |
| PC-3p-17914_31 | 1.948 567 463 | 0.001 313 257 | Up |
| PC-3p-23460_18 | 2.569 135 000 | 0.001 540 719 | Up |
| chi-miR-191-3p | -0.807 459 348 | 0.003 157 484 | Down |
| bta-miR-2377 | -0.723 000 000 | 0.005 163 673 | Down |
| oan-miR-16b-5p_L+1 | 2.369 133 16 | 0.006 208 570 | Up |
| bta-miR-2285i | 0.356 148 451 | 0.009 318 871 | Up |
| PC-5p-8555_99 | -1.033 720 831 | 0.010 246 079 | Down |
| PC-3p-20561_24 | -2.301 896 470 | 0.010 361 880 | Down |
| chi-miR-18a-3p_R+1 | -0.403 969 492 | 0.010 398 300 | Down |
| pal-miR-9993a-3p | -0.330 814 089 | 0.010 453 945 | Down |
| bta-miR-20b_R+3_1ss10CT | 0.719 604 509 | 0.020 103 455 | Up |
| bta-miR-215 | 2.408 603 805 | 0.030 145 620 | Up |
| mmu-miR-378d_R+2_1ss6CA | 1.752 421 816 | 0.035 169 227 | Up |
| bta-miR-362-5p | 0.584 875 060 | 0.038 184 233 | Up |
| eca-miR-1839_L-1R+3 | 2.094 428 940 | 0.042 436 847 | Up |
| PC-3p-13080_52 | -0.486 472 783 | 0.049 297 031 | Down |
| PC-3p-19898_25 | 2.022 363 047 | 0.050 328 524 | Up |
| bta-miR-500_R+2 | 0.370 279 491 | 0.050 354 398 | Up |
| bta-miR-219b-3p | 1.410 831 312 | 0.050 380 862 | Up |
| lca-miR-19b_R+2 | 1.588 370 285 | 0.050 393 992 | Up |
| bta-miR-146b | 0.729 240 479 | 0.050 461 322 | Up |
| PC-3p-17661_32 | 1.303 327 519 | 0.050 485 736 | Up |
Fig.3
Differential expression analysis of microRNAs"
Fig.4
Comparison of RT-qPCR results of microRNAs with RNA-Seq sequencing resultsA. β-Actin was used as internal reference；B. U6 was used as internal reference"
Table 4
GO functional annotation of differentially expressed microRNA"
功能组 Functionalgroup | 分类 Class | GO编号 GO ID | 显著基因数 Significantgenes | 总基因数 Annotated genes |
|---|---|---|---|---|
生物学过程 Biologicalprocess | 蛋白磷酸化 Protein phosphorylation | GO：0006468 | 172 | 3 590 |
RNA聚合酶Ⅱ转录正调控 Positive regulation of transcription by RNA polymerase II | GO：0045944 | 223 | 3 590 | |
细胞增殖负调控 Negative regulation of cell population proliferation | GO：0008285 | 78 | 3 590 | |
| 子宫胚胎发育 In utero embryonic development | GO：0001701 | 62 | 3 590 | |
| 囊泡运输 Vesicle-mediated transport | GO：0016192 | 48 | 3 590 | |
转化生长因子β受体信号通路 Transforming growth factor beta receptor signaling pathway | GO：0007179 | 28 | 3 590 | |
RNA聚合酶Ⅱ转录负调控 Negative regulation of transcription by RNA polymerase II | GO：0000122 | 150 | 3 590 | |
| 中心体定位 Centrosome localization | GO：0051642 | 11 | 3 590 | |
磷脂酰肌醇去磷酸化 Phosphatidylinositol dephosphorylation | GO：0046856 | 14 | 3 590 | |
| 转录正调控 Positive regulation of transcription | GO：0045893 | 123 | 3 590 | |
| 凋亡正调控 Positive regulation of apoptotic process | GO：0043065 | 58 | 3 590 | |
| 血生成 Hemopoiesis | GO：0030097 | 22 | 3 590 | |
| 细胞生长的负调控 Negative regulation of cell growth | GO：0030308 | 31 | 3 590 | |
| BMP信号通路 BMP signaling pathway | GO：0030509 | 26 | 3 590 | |
| 蛋白质转运 Protein transport | GO：0015031 | 45 | 3 590 | |
细胞组分 Cellularcomponent | 细胞溶质 Cytosol | GO：0005829 | 657 | 3 590 |
| 核质 Nucleoplasm | GO：0005654 | 624 | 3 590 | |
| 细胞质 Cytoplasm | GO：0005737 | 700 | 3 590 | |
| 细胞核 Nucleus | GO：0005634 | 725 | 3 590 | |
| 质膜 Plasma membrane | GO：0005886 | 505 | 3 590 | |
| 内质网 Endoplasmic reticulum | GO：0005783 | 200 | 3 590 | |
| 高尔基体 Golgi apparatus | GO：0005794 | 187 | 3 590 | |
胞内膜结合细胞器 Intracellular membrane-bounded organelle | GO：0043231 | 177 | 3 590 | |
| 内质网膜 Endoplasmic reticulum membrane | GO：0005789 | 93 | 3 590 | |
| 膜 Membrane | GO：0016020 | 481 | 3 590 | |
分子功能 Molecularfunction | 相同蛋白结合 Identical protein binding | GO：0042802 | 302 | 3 590 |
| 蛋白结合 Protein binding | GO：0005515 | 639 | 3 590 | |
| 蛋白激酶活性 Protein kinase activity | GO：0004672 | 142 | 3 590 | |
| 蛋白激酶结合 Protein kinase binding | GO：0019901 | 98 | 3 590 | |
| 泛素蛋白连接酶结合 Ubiquitin protein ligase binding | GO：0031625 | 73 | 3 590 | |
| ATP结合 ATP binding | GO：0005524 | 264 | 3 590 | |
| 序列特异性DNA结合 Sequence-specific DNA binding | GO：0043565 | 102 | 3 590 |
Fig.5
KEGG enrichment analysis of DEmicroRNA target genes"
| [1] | RUEGG P L.A 100-Year Review：Mastitis detection，management，and prevention［J］.J Dairy Sci，2017，100（12）：10381-10397. |
| [2] | 蔡明玉，张海龙，海珍珍，等.重组克柔念珠菌14-3-3蛋白诱导奶牛乳腺上皮细胞炎症反应的分子机制［J］.畜牧兽医学报，2023，54：1679-1689. |
| CAI M，ZHANG H L，HAI Z Z，et al.The inflamed mechanism induced by recombined 14-3-3 protein of Candida krusei on bovine mammary epithelial cells［J］.Acta Veterinaria et Zootechnica Sinica，2023，54：1679-1689.（in Chinese） | |
| [3] | 杜 军.克柔念珠菌两相损伤奶牛乳腺上皮细胞机制研究［D］.银川：宁夏大学，2021. |
| DU J.Study on the mechanism of two-phase injury of bovine mammary epithelial cells by Candida krusei［D］.Yinchuan：Ningxia University，2021.（in Chinese） | |
| [4] | GORJI A E，ROUDBARI Z，SADEGHI B，et al.Transcriptomic analysis on the promoter regions discover gene networks involving mastitis in cattle［J］.Microbial Pathog，2019，137：103801. |
| [5] | JU Z H，JIANG Q，LIU G，et al.Solexa sequencing and custom microRNA chip reveal repertoire of microRNAs in mammary gland of bovine suffering from natural infectious mastitis［J］.Anim Genet，2018，9（1）：3-18. |
| [6] | 杨 箭，王兴平，罗仍卓么，等.miRNA在奶牛乳房炎中的表达模式和分子调控机制［J］.农业生物技术学报，2020，28：2069-2079. |
| YANG J，WANG X P，LUORENG Z M，et al.The expression pattern and molecular regulatory mechanism of miRNA in mastitis of dairy cows（Bos taurus）［J］.Journal of Agricultural Biotechnology，2020，28：2069-2079.（in Chinese） | |
| [7] | SUN K，LAI E C.Adult-specific functions of animal microRNAs［J］.Nat Rev Genet，2013，14（8）：535-548. |
| [8] | NORMA S，ISABELLE D R，ISABELLE T，et al.MicroRNAs in inflammasomopathies［J］.Immunol Lett，2023，256-257：48-54. |
| [9] | RYAN M O，DINESH S R，C，AADEL A，et al.Physiological and pathological roles for microRNAs in the immune system［J］.Nat Rev Immunol，2010，10（2）：111-122. |
| [10] | SATOSHI N，YA C，MASAYUKI S，et al.Transcriptomic profiling identifies an exosomal microRNA signature for predicting recurrence following surgery in patients with pancreatic ductal adenocarcinoma［J］.Ann Surg，2022，276（6）：e876-e885. |
| [11] | DAMIEN F，RONAN G S，LOUISE B，et al.The identification of circulating miRNA in bovine serum and their potential as novel biomarkers of early Mycobacterium avium subsp paratuberculosis infection［J］.PloS One，2015.10（7）：e0134310. |
| [12] | LI R，ZHANG C L，LIAO X，et al.Transcriptome microRNA profiling of bovine mammary glands infected with Staphylococcus aureus［J］.Int J Mol Sci，2015，16（3）：4997-5013. |
| [13] | LAI Y C，TAKURO F，TADASHI M，et al.Inflammation-related microRNA expression level in the bovine milk is affected by mastitis［J］.PloS One，2017，12（5）：e0177182. |
| [14] | LUORENG Z M，WANG X P，MEI C G，et al.Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis［J］.Sci Rep，2018，8（1）：12693. |
| [15] | WANG X Z，SU F，YU X H，et al.RNA-seq whole transcriptome analysis of bovine mammary epithelial cells in response to intracellular Staphylococcus aureus［J］.Front Vet Sci，2020，7：642. |
| [16] | LUORENG Z M，WEI D W，WANG X P.MiR-125b regulates inflammation in bovine mammary epithelial cells by targeting the NKIRAS2 gene［J］.Vet Res，2021，52（1）：122. |
| [17] | BAGNICKA E，KAWECKA-GROCHOCKA E，PAWLINA-TYSZKO K，et al.MicroRNA expression profile in bovine mammary gland parenchyma infected by coagulase-positive or coagulase-negative staphylococci［J］.Vet Res，2021，52（1）：41. |
| [18] | PU J H，LI R，ZHANG C L，et al.Expression profiles of miRNAs from bovine mammary glands in response to Streptococcus agalactiae-induced mastitis［J］.J Dairy Res，2017，84（3）：300-308. |
| [19] | MA S Y，TONG C，EVELINE M I A，et al.Identification and characterization of differentially expressed exosomal microRNAs in bovine milk infected with Staphylococcus aureus［J］.BMC Genomics，2019，20（1）：934. |
| [20] | MAN Z，HERMAN W B，JIAN G，et al.MicroRNA miR-223 modulates NLRP3 and Keap1，mitigating lipopolysaccharide-induced inflammation and oxidative stress in bovine mammary epithelial cells and murine mammary glands［J］.Vet Res，2023，54（1）：78. |
| [21] | CHEN Z，LIANG Y，LU Q，et al.Cadmium promotes apoptosis and inflammation via the circ08409/miR-133a/TGFB2 axis in bovine mammary epithelial cells and mouse mammary gland［J］.Ecotoxicol Environ Saf，2021，222：112477. |
| [22] | YU C，JING Y，ZHI H，et al.Exosomal lnc-AFTR as a novel translation regulator of FAS ameliorates Staphylococcus aureus-induced mastitis［J］.BioFactors （Oxford，England），2022，48（1）：148-163. |
| [23] | 潘晓乐.奶牛金黄色葡萄球菌性乳腺炎免疫相关microRNA的筛选及其生物学功能 ［D］.银川：宁夏大学，2022. |
| PAN X L.Screening og microRNAs in immune response to S.aureus isolated from bovine mastitis and their biological functions［D］.Yinchuan ：Ningxia University，2022.（in Chinese） | |
| [24] | ZHANG Y P，XU Y Q，CHEN B W，et al.Selenium deficiency promotes oxidative stress-induced mastitis via activating the NF-κB and MAPK pathways in dairy cow［J］.Biol Trace Elem Res，2022，200（6）：2716-2726. |
| [25] | LI J D，YIN P，GONG P，et al.8-methoxypsoralen protects bovine mammary epithelial cells against lipopolysaccharide-induced inflammatory injury via suppressing JAK/STAT and NF-κB pathway［J］.Microbiol Immunol，2019，63（10）：427-437. |
| [26] | MARVALIM C，DATTA A，LEE S C.Role of p53 in breast cancer progression：An insight into p53 targeted therapy［J］.Theranostics，2023，13（4）：1421-1442. |
| [27] | XU F，NA L X，LI Y F，et al.Roles of the PI3K/AKT/mTOR signalling pathways in neurodegenerative diseases and tumours［J］.Cell Biosci，2020，10（1）：54. |
| [28] | SEYED H A，KHALIL K，MEHRAN G.Toll-like receptors （TLRs） and their potential therapeutic applications in diabetic neuropathy［J］.Int Immunopharmacol，2022.102：108398. |
| [29] | MOSTAFIZAR M，CORTES-PÉREZ C，SNOW W，et al.Challenges with methods for detecting and studying the transcription factor nuclear factor kappa B （NF-κB） in the central nervous system［J］.Cells，2021，10（6）：1335. |
| [30] | 王 凡.miR-148a靶向TLR4/NF-κB调控奶牛乳腺上皮细胞炎性损伤的研究［D］.杨凌：西北农林科技大学，2024. |
| WANG F.MiR-148a targets TLR4/NF-κB pathway to attenuate inflammatory injury in bovine mammary epithelial cells ［D］.Yangling ：Northwest A & F University，2024 （in Chinese） | |
| [31] | MOCKENHAUPT K，GONSIEWSKI A，KORDULA T.RelB and neuroinflammation［J］.Cells，2021，10（7）：1609. |
| [32] | CILDIR G，LOW K C，TERGAONKAR V.Noncanonical NF-κB signaling in health and disease［J］.Trend Mol Med，2016，22（5）：414-429. |
| [33] | MILLET P，MCCALL C，YOZA B.RelB：an outlier in leukocyte biology［J］.J Leukoc Biol，2013，94（5）：941-951. |
| [34] | 吴钟伟，赵圣吉，李春富，等.miR-146a-3p靶向RELB调控动脉粥样硬化小鼠内皮细胞的细胞因子分泌 ［J］.海南医学院学报，2020，26：742-748. |
| WU Z W，ZHAO S J，LI C F，et al.miR-146a-3p targets RELB regulates cytokine secretion in endothelial cells of atherosclerotic mice［J］.Journa of Hainan Medical Colleage，2020，26：742-748.（in Chinese） | |
| [35] | MA Y，JADE N.Specificity models in MAPK cascade signaling［J］.FEBS Open Bio，2023，13（7）：1177-1192. |
| [36] | AIWEN S，LIU L，LI S，et al.Natural products targeting the MAPK-signaling pathway in cancer：overview［J］.J Cancer Res Clin Oncol，2024，150（1）：6. |
| [37] | WANG H，BI C L，WANG Y J，et al.Selenium ameliorates Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells by inhibiting activation of TLR2，NF-κB and MAPK signaling pathways［J］.BMC Vet Res，2018.14（1）：197. |
| [38] | XU P，ZHANG G F，HOU S X，et al.MAPK8 mediates resistance to temozolomide and apoptosis of glioblastoma cells through MAPK signaling pathway［J］.Biomed Pharmacother，2018.106：1419-1427. |
| [39] | GU M，LIU K，XIONG H，et al.MiR-130a-3p inhibits endothelial inflammation by regulating the expression of MAPK8 in endothelial cells［J］.Heliyon，2024，10（2）：e24541. |
| [40] | HU Q，BIAN Q H，RONG D C，et al.JAK/STAT pathway：Extracellular signals，diseases，immunity，and therapeutic regimens［J］.Front Bioeng Biotechnol，2023，11：1110765. |
| [41] | NITISH K，NIDHI S，SIDHARTH M.Connection between JAK/STAT and PPARγ signaling during the progression of multiple sclerosis：Insights into the modulation of T-cells and immune responses in the brain［J］.Curr Mol Pharmacol，2021，14（5）：823-837. |
| [42] | HUANG W，LI Y Y，ZHANG C，et al.IGF2BP3 facilitates cell proliferation and tumorigenesis via modulation of JAK/STAT signalling pathway in human bladder cancer［J］.J Cell Mol Med，2020，24（23）：13949-13960. |
| [43] | 蒲俊华.奶牛链球菌型乳腺炎乳腺组织基因表达与microRNA分析及miR-122对EPO和JAK-STAT通路靶向调控 ［D］.扬州：扬州大学，2017. |
| PU J H.Expression profiles of genes and microRNAs from bovine mammary glands in response to Streptococcus agalatiae-induced mastitis and regulation of miR-122 on EPO and JAK-STAT pathway［D］.Yangzhou：Yangzhou University，2017.（in Chinese） | |
| [44] | RUCHI P A，SCOTT M L，RAZELLE K.JAK：Not just another kinase［J］.Mol Cancer Therap，2022，21（12）：1757-1764. |
| [45] | AWASTHI N，LIONGUE C，C，WARD A.STAT proteins：a kaleidoscope of canonical and non-canonical functions in immunity and cancer［J］.J Hematol Oncol，2021，14（1）：198. |
| [46] | MAURER B，KOLLMANN S，PICKEM J，et al.STAT5A and STAT5B-twins with different personalities in hematopoiesis and leukemia［J］.Cancers，2019，11（11）：1726. |
| [47] | GOTTHARDT D，TRIFINOPOULOS J，SEXL V，et al.JAK/STAT cytokine signaling at the crossroad of NK cell development and maturation［J］.Front Immunol，2019，10：2590. |
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