金黄色葡萄球菌噬菌体脂质体的制备及胞内抗菌活性

doi:10.11843/j.issn.0366-6964.2025.09.041

Abstract

Abstract:

The aim of this study was to investigate the use of liposomes as a carrier to deliver phage into the cell and the antibacterial effect of phage liposomes on Staphylococcus aureus in bovine mammary epithelial cells. In this study, liposome-encapsulated phage SDQ was prepared using the thin-film hydration method. The acid-base stability and thermal stability of the phage liposomes were determined using the double-layer plate method. The particle size, polydispersity coefficient, and Zeta potential of phage liposomes were measured using a laser particle size analyzer, while the structural characteristics of the phage liposomes were observed through transmission electron microscopy. The effect of phage liposomes on the viability of MAC-T cells was determined using the CCK-8 assay. The efficiency of cell entry and the intracellular and bactericidal activity of phage liposomes were measured using the plate counting method. The results showed that liposomes made from soybean lecithin, cholesterol, Tween 80, and octadecylamine were prepared in a ratio of 8:2:1:0.5, and phage liposomes (Lip-p-7) were created by encapsulating phage SDQ. The encapsulation rate of Lip-p-7 was greater than 50%, and its activity is stable in the range of temperature (4-60 ℃) and pH value (2-11). The average particle size of Lip-p-7 was 1 262.5 nm, and the shift of Zeta potential from neutral to negatively charged signified the successful encapsulation of phage SDQ by the liposome. The polydispersity index (PDI) was less than 0.7, suggesting a uniform dispersion. When stored at 4 ℃, the prepared Lip-p-7 could maintain its intact activity for up to 90 days, demonstrating good stability. Liposomes can effectively increase the number of phage SDQ entering cells and decrease the number of Staphylococcus aureus in MAC-T cells. In conclusion, this study provides a reference for the treatment of intracellular bacterial infections by using liposomes as a carrier to deliver bacteriophages into the cells.

Key words: liposomes, bacteriophage, Staphylococcus aureus, MAC-T cells, intracellular antibacterial activity

CLC Number:

Q939.48

YANG Shubo, YUAN Qingxin, CHEN Qibai, WANG Pei, GAO Dongyang, LI He, SONG Jun. Preparation and Intracellular Antibacterial Activity of Liposomes Encapsulating Staphylococcus aureus Bacteriophage[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(9): 4638-4645.

Figures/Tables 8

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References 28

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样本 Sample	大豆卵磷脂: 胆固醇: 吐温80:十八胺 PC: Chol: Tw-80: ODA	温度/℃ Temperature	酸碱度 pH value	包封率/% Encapsulation rate
Lip-p-1	6:4:2:0	4~70	4~10	10.98
Lip-p-2	8:4:2:0	4~60	3~10	22.66
Lip-p-3	8:4:2:0.5	4~70	3~11	35.51
Lip-p-4	6:4:2:0.5	4~50	2~10	23.65
Lip-p-5	6:2:1:0.5	4~60	2~11	38.72
Lip-p-6	8:2:1:1	4~50	3~11	53.28
Lip-p-7	8:2:1:0.5	4~60	2~11	50.37
Lip-p-8	6:1:1:1	4~60	3~11	19.73
Lip-p-9	8:1:1:1	4~60	3~11	22.48

脂质体 Liposomes	平均粒径(${\bar x}$±s)/nm Average particle size /nm	多分散系数 Polydispersity coefficient	Zeta电位(${\bar x}$±s)/mV Zeta potential /mV
Lip	656.75±127.40	0.505	44.40±9.38
Lip-p-7	1 262.50± 200.70	0.675	-13.70±7.20

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Preparation and Intracellular Antibacterial Activity of Liposomes Encapsulating Staphylococcus aureus Bacteriophage

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