基于Smart-seq2探究玻璃化冷冻对猪孤雌激活囊胚基因表达的影响

doi:10.11843/j.issn.0366-6964.2024.09.018

Abstract

Abstract:

The aim of this study was to investigate the effect of vitrification on gene expression in porcine parthenogenetic activation (PA) blastocysts. In this study, porcine PA blastocysts were divided into fresh, vitrification group Ⅰ and vitrification group Ⅱ according to the experimental treatments, and then 3 well-morphologised blastocysts were selected from each group and analysed by transcriptome sequencing using Smart-seq2 single-cell full-length transcriptome sequencing technology. As a result, compared with the fresh group, a total of 772 differential expression genes (DEGs) were identified in the vitrification group Ⅰ. GO and KEGG enrichment analysis results showed that these DEGs were related to cellular lipid metabolic process, cell glucose homeostasis, MAPK signaling pathway, PI3K-Akt signaling pathway, etc. Compared with the fresh group, a total of 1 613 DEGs were identified in the vitrification group Ⅱ, which mainly related to gluconeogenesis, metabolic pathways, biosynthesis of amino acids, etc. Compared with the vitrification group Ⅰ, 822 DEGs were identified in the vitrification group Ⅱ, which mainly involved in pyruvate metabolic process, N-Glycan biosynthesis, cellular senescence, etc. In summary, this study revealed the effects of vitrification on the expression of genes related to lipid metabolism, energy metabolism, MAPK signalling pathway and other related genes in porcine PA blastocysts based on Smart-seq2 single-cell full-length transcriptome sequencing technology.

Key words: Smart-seq2, vitrification, pig, parthenogenetic activation, blastocyst

CLC Number:

S828.3

Baigao YANG, Xi LONG, Liang ZHANG, Jiehuan XU, Jianjun DAI, Xueming ZHAO, Hongmei PAN. Exploring the Effect of Vitrification on Gene Expression in Porcine Parthenogenetic Blastocysts by Smart-seq2[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3936-3946.

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基因ID Gene ID	基因名称 Gene name	log₂(FC)	P值 P value	上调或下调 Up or Down
ENSSSCG00000003333	C1QTNF12	20.68	8.76×10^-14	UP
ENSSSCG00000049746	ENSSSCG00000049746	6.06	6.16×10^-8	UP
ENSSSCG00000052366	ENSSSCG00000052366	9.46	7.49×10^-8	UP
ENSSSCG00000061727	ENSSSCG00000061727	5.22	1.79×10^-7	UP
ENSSSCG00000059571	ENSSSCG00000059571	9.77	2.80×10^-7	UP
ENSSSCG00000032135	ENSSSCG00000032135	-7.10	1.16×10^-7	Down
ENSSSCG00000008294	ACTG2	-8.19	3.51×10^-7	Down
ENSSSCG00000011657	CLDN18	-7.29	2.41×10^-5	Down
ENSSSCG00000022774	NOP53	-3.09	3.67×10^-5	Down
ENSSSCG00000038718	ADRM1	-4.35	5.93×10^-5	Down

基因ID Gene ID	基因名称 Gene name	log₂(FC)	P值 P value	上调或下调 Up or Down
ENSSSCG00000045128	ENSSSCG00000045128	6.11	2.10×10^-28	UP
ENSSSCG00000008801	SLC30A9	4.32	4.49×10^-27	UP
ENSSSCG00000041449	ENSSSCG00000041449	5.41	2.77×10^-24	UP
ENSSSCG00000013448	MKNK2	4.68	2.05×10^-23	UP
ENSSSCG00000055081	ENSSSCG00000055081	7.55	1.98×10^-21	UP
ENSSSCG00000036649	FAM151A	-4.30	2.03×10^-19	Down
ENSSSCG00000007984	MRPL28	-3.25	5.21×10^-18	Down
ENSSSCG00000016461	ZYX	-3.31	2.16×10^-16	Down
ENSSSCG00000003153	FTL	-4.07	2.45×10^-16	Down
ENSSSCG00000028197	PFKL	-3.74	1.15×10^-14	Down

基因ID Gene ID	基因名称 Gene name	log₂(FC)	P值 P value	上调或下调 Up or Down
ENSSSCG00000059025	RAB21	2.93	4.92×10^-6	UP
ENSSSCG00000036491	PCNP	2.79	1.76×10^-5	UP
ENSSSCG00000031337	SNX18	3.18	1.89×10^-5	UP
ENSSSCG00000008801	SLC30A9	3.03	2.45×10^-5	UP
ENSSSCG00000028737	USP3	2.86	3.49×10^-5	UP
ENSSSCG00000049267	ENSSSCG00000049267	-21.72	1.57×10^-11	Down
ENSSSCG00000051983	ENSSSCG00000051983	-21.68	1.81×10^-11	Down
ENSSSCG00000038832	U2	-21.63	2.49×10^-11	Down
ENSSSCG00000063160	ENSSSCG00000063160	-21.63	2.49×10^-11	Down
ENSSSCG00000051866	ENSSSCG00000051866	-21.61	2.73×10^-11	Down

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Exploring the Effect of Vitrification on Gene Expression in Porcine Parthenogenetic Blastocysts by Smart-seq2

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