Table 1

qRT-PCR primers sequence"

Fig. 1

Purification identification and growth characteristics of DCMECs A. Primary culture and purification of DCMECs; B. CK-18 identification of DCMECs; C. Cell growth curve"

Fig. 2

Effects of exogenous CTGF addition on cell proliferation and apoptosis A.EdU was used to detect cell proliferation, DAPI was the stained nucleus, and red fluorescence was the proliferating cells; B. Apoptosis was detected by flow cytometry; C. Fluorescent quantitative PCR was used to detect the marker genes expression of cell proliferation and apoptosis; D. Western blot was used to detect proteins expression related to cell proliferation and apoptosis synthesis. * the difference was significant (P < 0.05); ** the difference was very significant (P < 0.01), the same as below"

Fig. 3

Effects of overexpression of CTGF on cell proliferation and apoptosis A.EdU was used to detect cell proliferation, DAPI was the stained nucleus, and red fluorescence was the proliferating cells; B. Apoptosis was detected by flow cytometry; C. Fluorescent quantitative PCR to detect the marker genes expression of cell proliferation and apoptosis; D. Western blot was used to detect proteins expression related to cell proliferation and apoptosis synthesis. Different lowercase letters indicated significant difference (P<0.05); Same lowercase letter means no significant difference (P>0.05), the same as below"

Fig. 4

Effect of exogenous addition of CTGF on milk protein synthesis in cells A. Intracellular milk fat synthesis was detected by Nile red staining, DAPI was the stained nucleus, and red fluorescence was the amount of milk fat synthesis; B. The effect of adding CTGF on the content of synthesized triglyceride (TAG); C. Fluorescent quantitative PCR detection of milk fat synthesis marker genes expression; D. Fluorescent quantitative PCR was used to detect the marker genes expression of milk protein synthesis; E. Western blot was used to detect proteins expression related to milk fat synthesis; F. Western blot was used to detect the expression of proteins related to milk protein synthesis in cells"

Fig. 5

Effect of endogenous overexpression of CTGF on proteins synthesis of cellular milk fat A. Intracellular milk fat synthesis was detected by Nile red staining, DAPI was the stained nucleus, and red fluorescence was the amount of milk fat synthesis; B. Effect of overexpression of CTGF on the content of triglyceride (TAG) secreted by cells in culture medium; C. Fluorescent quantitative PCR detection of milk fat synthesis marker genes expression; D. Fluorescent quantitative PCR was used to detect the marker genes expression of milk protein synthesis; E. Western blot was used to detect proteins expression related to milk fat synthesis; F. Western blot was used to detect the expression of protein related to milk protein synthesis in cells"

Fig. 6

Changes of ITGB1 gene and protein expression corresponding to induced lactation differentiation when exogenous CTGF was added A. qRT-PCR to detect ITGB1 gene expression; B. Western blot detected ITGB1 protein expression"

Fig. 7

Changes of ITGB1 gene and protein expression corresponding to induced lactation differentiation with CTGF overexpression A. qRT-PCR to detect ITGB1 gene expression; B. Western blot was used to detect ITGB1 protein expression"

Fig. 8

Interaction between CTGF and β1 integrin identified by Co-IP"