单细胞转录组学技术及其在寄生虫研究中的应用进展

doi:10.11843/j.issn.0366-6964.2024.10.005

Abstract

Abstract:

The single cell transcriptome represents the total expression of all mRNA in a single cell at a certain time, and its expression level reflects the overall characteristics of the cell. The entire RNA of a single cell is reversely transcribed, amplified and sequenced by single cell RNA sequencing (scRNA-seq). The extensive information contained in the sequencing results has the potential to reshape the understanding of developmental biology, gene regulation and cellular heterogeneity in health and disease. With the continuous development of science and technology, sequencing technologies are also constantly improving. The omni-directional, high-throughput and high-resolution characteristics of scRNA-seq can build a more detailed, comprehensive and accurate single cell map. This paper summarizes the commonly used scRNA-seq methods, the main processes of data analysis, and the application of single cell transcriptome technology in the field of parasitology and parasitic diseases, in order to provide reference materials for related research in this field.

Key words: scRNA-seq, transcriptome, data analysis, parasitic diseases

CLC Number:

Tingting ZHOU, Li LI, Yantao WU, Wenying LU, Baoquan FU, Hong YIN, Wanzhong JIA, Hongbin YAN. Progress on Single-cell Transcriptomics Technology and Its Applications in Research on Parasites[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(10): 4290-4301.

Figures/Tables 1

References 82

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测序技术	单细胞分离技术	转录本覆盖范围	优势	扩增	唯一分子标识符
Sequencing technology	Single cell separation	Transcript coverage	Advantage	Amplification	UMI
CEL-seq/CEL-seq2	手动稀释分离	3′端序列	线性扩增以高精度减少非特异性片段的积累	IVT	有
MARS-seq/MARS-seq2	荧光激活细胞分选	3′端序列	高通量，良好的稳定性	IVT	有
SMART-seq/SMART-seq2	手动稀释分离	全长	cDNA文库的平均长度和产量升高，序列覆盖率较高	PCR	无
SMART-seq3	荧光激活细胞分选	全长	高灵敏度，能够较好地识别细胞类型、状态及亚型特异性	PCR	有
SMART-seq3xpress	荧光激活细胞分选	全长	高通量，耗时短，低成本	PCR	有
SCRB-seq	荧光激活细胞分选	3′端序列	高通量，低成本	PCR	有
Drop-seq	微滴技术	3′端序列	高通量，低成本	PCR	有
inDrop	微流体技术	3′端序列	高通量，低成本	IVT	有
10×Genomics	微流体技术	3′端序列	高通量，低成本，高灵敏度	PCR	有
Microwell-seq	琼脂糖微孔阵列	全长	高通量，低成本	PCR	无
Seq-well	微孔阵列	3′端序列	高通量，交叉污染较小	PCR	有
Live-seq	流体力显微镜技术	全长	保留细胞生物活性	PCR	无

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Progress on Single-cell Transcriptomics Technology and Its Applications in Research on Parasites

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