荣昌猪初产繁殖性状的全基因组关联研究

doi:10.11843/j.issn.0366-6964.2023.01.010

Abstract

Abstract: The aim of this study was to identify genetic variant loci and genes which associated with reproductive traits at first farrowing in Rongchang pigs, and provide important molecular markers and genes for improving pig reproductive traits in Rongchang pigs. In this study, a total of 429 Rongchang sows were genotyped using porcine 50K SNP chip. After quality control, 35 046 SNPs were generated for subsequent genome-wide association study (GWAS). Then, the population structure was analyzed using principal component analysis, and GWAS was performed for total number born (TNB), total number born alive (NBA), number of stillborn (NS) and litter weight born alive (LWB) using a mixed-linear model (MLM). The birth year and birth month were used as fixed effects, and principal component values were used as a covariate in this model. The results showed that a total of 2 and 1 genome-wide significant SNPs were detected for LWB and NS, respectively. Furthermore, a total of 5, 3 and 10 suggestive SNPs were found for TNB, NBA and NS, respectively. Of these, one locus located on SSC17 (position: 57 315 180 bp) were evaluated to affect TNB, NBA and LWB and one locus located on SSC1 (position: 279 214 647 bp) were evaluated to affect both NBA and TNB, indicating the pleiotropism of genes in different reproductive traits. Considering the function of candidate genes, BMP7 gene was suggested as the promising candidate genes for TNB, NBA and LWB, and two genes(MSH3 and CBLB) were suggested as the important candidate genes for NS. These results provided novel variant loci and potential candidate genes for reproductive traits, and provided important theoretical basis for improving reproductive traits using genomic selection in Rongchang pigs.

Key words: Rongchang pig, GWAS, number of piglets, number of stillborn, litter weight born alive

CLC Number:

S828.2

WU Pingxian, CHEN Li, LONG Xi, CHAI Jie, ZHANG Tinghuan, XU Shunlai, GUO Zongyi, WANG Jinyong. Genome-wide Association Studies for Reproductive Traits at First Farrowing in Rongchang Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 103-112.

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Genome-wide Association Studies for Reproductive Traits at First Farrowing in Rongchang Pigs

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