基于全基因组填充重测序关联分析鉴别影响苏山猪初生体尺与乳头数性状的遗传位点

doi:10.11843/j.issn.0366-6964.2023.01.009

Abstract

Abstract: The purpose of this study was to identify the genetic loci that affected the body conformation at birth and teat number traits, develop molecular markers that can be used for assisted breeding, and provide a theoretical basis for the continuous selection of growth and reproductive performance in Sushan pig population. The phenotype included body conformation at birth and teat number were measured in 269 Sushan newborn piglets (within 24 h after birth), and the ear tissue samples were collected at the same time. Based on whole-genome resequencing and genotype imputation strategy, GWAS and Fst methods were used to identify the significant loci associated with the target traits. GO and KEGG analyses were performed on the positional and functional candidate genes to identify the most likely major genes. A total of 4 candidate loci were identified in this study, located in the 13, 14 and X chromosomes, of which 2 were significantly associated with body conformation at birth traits, and 2 were significantly associated with the teat number traits. This study screened out 1 candidate gene ACTA2 related to the body length, 1 candidate gene COL4A6 related to the chest circumference, and 3 candidate genes ACTA2, CRTAP, and SEPTIN6 related to the teat number. This research provides important molecular markers for the genetic improvement of body conformation at birth and teat number traits in Sushan pig population.

Key words: Sushan pig, body conformation at birth, teat number, GWAS, candidate genes

CLC Number:

S828.2

ZHANG Changzheng, LI Desen, HUANG Min, FANG Xiaomin, ZHAO Weimin, REN Shouwen, DONG Huansheng, REN Jun, ZHOU Lisheng. An Imputed Whole-genome Sequence-based GWAS Approach Pinpoints Genetic Loci for Body Conformation at Birth and Teat Number Traits in Sushan Pig Population[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 88-102.

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An Imputed Whole-genome Sequence-based GWAS Approach Pinpoints Genetic Loci for Body Conformation at Birth and Teat Number Traits in Sushan Pig Population

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