硒对树突状细胞和巨噬细胞功能的调控作用

doi:10.11843/j.issn.0366-6964.2022.09.037

Abstract

Abstract: The aim of this study was to investigate the effects of selenium on dendritic cells (DCs) and macrophages. Selenium was cultured with bone marrow derived dendritic cells (BMDCs) and peritoneal macrophages, respectively. Flow cytometry was used to detect phagocytosis activity of immature BMDCs and macrophage, as well as the phenotype MHCⅡ, CD86, CD80 and CD40 expression on BMDCs, and the ability of BMDCs treated with selenium to stimulate the proliferation and antigen presenting of allogenic lymphocytes. The cytokines (IL-12, IL-1β, IFN-γ, IL-6, IL-10, TNF-α, NO) in the supernatant of BMDCs and macrophages were detected by ELISA. The results showed that when the concentration of selenium was 0.18-0.09 mg·L^-1, the phagocytic activity of BMDCs and macrophages were significantly enhanced(P<0.05), and the expression levels of MHCⅡ, CD86 and CD80 on BMDCs were significantly increased(P<0.05), and the ability to stimulate the proliferation and antigen presentation of allogeneic lymphocytes was also significantly increased (P<0.05). The contents of IFN-γ, IL-12 and IL-10 were significantly increased in the supernatant of BMDCs(P<0.05), while the contents of IFN-γ, TNF-α and NO were significantly increased in the supernatant of macrophages(P<0.05). The results indicated that proper concentration of selenium could enhance the function of DCs and peritoneal macrophages, and it was worth further exploring the effect of selenium on immune function.

Key words: selenium, dendritic cells, macrophages

CLC Number:

S859.1

JING Lirong, ZHANG Chao, QIAO Jie, HAI Nisige, YANG Ying, GAO Zhenzhen. Effects of Selenium on the Function of Dendritic Cells and Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3231-3240.

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Effects of Selenium on the Function of Dendritic Cells and Macrophages

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