硒缺乏通过TLR4/MyD88/NF-κB信号通路介导鸡胸腺细胞凋亡

doi:10.11843/j.issn.0366-6964.2022.11.029

Abstract

Abstract: This study aimed to investigate whether selenium deficiency induced apoptosis of chicken thymocytes by activating toll-like receptor signaling pathway. In vivo experiment, 200 healthy one-day-old broilers were randomly divided into control group (C group) and selenium deficiency group (L group) by replicating selenium deficiency model, the control group was fed a normal diet with 0.2 mg·kg^-1 selenium, and the se-deficient group was fed a se-deficient diet with 0.004 mg·kg^-1 selenium. At 15, 25, 35, 45, and 55 days of age, 15 chicks were selected from each group, the morphological and ultrastructural changes of thymus tissue were observed. TLR4 signaling pathway and apoptosis-related factors were detected in thymus tissue. The selenium deficiency model of MDCC-MSB1(MSB1) cells in vitro was established, and six groups were established on this basis:Control (C) group, Selenium deficiency (L) group, selenium deficiency+transfection empty plasmid (L+pCMV-HA-N) group, selenium deficiency+siRNA negative control (L+ NCsiRNA) group, selenium deficiency + overexpression of TLR4 (L+ pCMV-HA-TLR4) group, selenium deficiency +siChTLR4 (L+ siChTLR4) group. After 5 days of low selenium treatment, cell viability, apoptosis, TLR4 signal transduction pathway and expression of apoptosis-related factors were detected. Results were as follows:1) Compared with the C group, the number of lymphocytes in the medulla of the cortex in the L group was reduced, the cells were disordered, the medulla of the cortex was hyperemic, the nucleus was fragmented, and extensive focal necrosis was observed. The thymus tissue of chickens in L group showed fissure, reduced volume, cell fragmentation, and nuclear chromatin edge aggregation, mitochondrial swelling, cristae fracture; 2) Compared with C group at 15 days of age, mRNA and protein expressions of TLR4, MyD88, NF-κB, Caspase-3, Caspase-9 and Bax in the thymus of L group were up-regulated at 15 to 55 days of age, the expression levels of Bcl-2 mRNA and protein showed opposite trends; 3) Compared with C group, the viability of MSB1 cells in L group was significantly decreased, the number of apoptosis was significantly increased, the mRNA and protein expressions of TLR4, MyD88, NF-κB, Caspase-3, Caspase-9 and Bax were significantly increased, and the mRNA and protein expression levels of Bcl-2 were significantly decreased. Compared with the L group, the cell viability was significantly enhanced, the number of apoptosis was significantly decreased, and the mRNA and protein expression of related factors were significantly decreased in the L+siChTLR4 group, while the cell viability was significantly decreased, the number of apoptosis was significantly increased, and the mRNA and protein expression of related factors were significantly increased in the L+ pCMV-HA-TLR4 group. In conclusion, selenium deficiency mediates the apoptosis of chicken thymocyte through the TLR4/MyD88/NF-κB signaling pathway, and further induces thymus injury in chickens.

Key words: selenium deficiency, TLR4, MyD88, NF-κB, thymus, apoptosis

CLC Number:

S852.3

ZHANG Ruili, ZHANG Di, GUO Rong, CHEN Yang, LI Guangxing, HUANG Xiaodan. Selenium Deficiency Induced Thymocytes Apoptosis of Broilers via the TLR4/MyD88/NF-κB Signaling Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4035-4047.

References 38

[1]	ROMAN M, JITARU P, BARBANTE C.Selenium biochemistry and its role for human health[J].Metallomics, 2014, 6(1):25-54.
[2]	HE S P, YU Q F, HE Y J, et al.Dietary resveratrol supplementation inhibits heat stress-induced high-activated innate immunity and inflammatory response in spleen of yellow-feather broilers[J].Poult Sci, 2019, 98(12):6378-6387.
[3]	ZHANG R L, GUO R, LIU Q, et al.Selenium deficiency via the TLR4/TRIF/NF-κB signaling pathway leading to inflammatory injury in chicken spleen[J].Biol Trace Elem Res, 2021, 199(2):693-702.
[4]	ZHANG R L, LIU Q, GUO R, et al.Selenium deficiency induces autophagy in chicken bursa of fabricius through ChTLR4/MyD88/NF-κB pathway[J].Biol Trace Elem Res, 2021, 200(7):3303-3314.
[5]	ZHANG L W, GAO Y H, FENG H Q, et al.Effects of selenium deficiency and low protein intake on the apoptosis through a mitochondria-dependent pathway[J].J Trace Elem Med Biol, 2019, 56:21-30.
[6]	ZHENG Y Y, ZHANG B, GUAN H Y, et al.Selenium deficiency causes apoptosis through endoplasmic reticulum stress in swine small intestine[J].Biofactors, 2021, 47(5):788-800.
[7]	KATAOKA K, TOKUTOMI Y, YAMAMOTO E, et al.Apoptosis signal-regulating kinase 1 deficiency eliminates cardiovascular injuries induced by high-salt diet[J].J Hypertens, 2011, 29(1):76-84.
[8]	SAITOH M, NISHITOH H, FUJII M, et al.Mammalian thioredoxin is a direct inhibitor of apoptosis signal-regulating kinase (ASK) 1[J].EMBO J, 1998, 17(9):2596-2606.
[9]	KRISHNAN J, SELVARAJOO K, TSUCHIYA M, et al.Toll-like receptor signal transduction[J].Exp Mol Med, 2007, 39(4):421-438.
[10]	TAKEDA K, AKIRA S.Toll-like receptors[M]//Current Protocols in Immunology.New York:John Wiley & Sons, Inc., 2015.
[11]	刘晴.ChTLR4/MyD88/NF-κB信号通路介导硒缺乏雏鸡法氏囊自噬发生的研究[D].哈尔滨:东北农业大学, 2021.LIU Q.ChTLR4/MyD88/NF-κB signaling pathway mediated autophagy in selenium-deficient chicken bursa of Fabricius[D].Harbin:Northeast Agricultural University, 2021.(in Chinese)
[12]	ZAREMBER K A, GODOWSKI P J.Tissue expression of human Toll-like receptors and differential regulation of Toll-like receptor mRNAs in leukocytes in response to microbes, their products, and cytokines[J].J Immunol, 2002, 168(2):554-561.
[13]	BERNASCONI P, BARBERIS M, BAGGI F, et al.Increased toll-like receptor 4 expression in thymus of myasthenic patients with thymitis and thymic involution[J].Am J Pathol, 2005, 167(1):129-139.
[14]	MIAO Z R, MIAO Z Y, WANG S C, et al.Exposure to imidacloprid induce oxidative stress, mitochondrial dysfunction, inflammation, apoptosis and mitophagy via NF-kappaB/JNK pathway in grass carp hepatocytes[J].Fish Shellfish Immunol, 2022, 120:674-685.
[15]	BISWAS S K, LOPEZ-COLLAZO E.Endotoxin tolerance:new mechanisms, molecules and clinical significance[J].Trends Immunol, 2009, 30(10):475-487.
[16]	任改艳, 孙阿宁, 张晶晶, 等.NF-κB在细胞凋亡中调节作用的研究进展[J].中国药理学与毒理学杂志, 2015, 29(2):323-327.REN G Y, SUN A N, ZHANG J J, et al.Advances in roles of NF-κB in regulating pathways of apoptosis[J].Chinese Journal of Pharmacology and Toxicology, 2015, 29(2):323-327.(in Chinese)
[17]	GAO J, LIU Q H, LI J L, et al.Fibroblast growth factor 21 dependent TLR4/MYD88/NF-κB signaling activation is involved in lipopolysaccharide-induced acute lung injury[J].Int Immunopharmacol, 2020, 80:106219.
[18]	HUANG X Q, QIAO F, XUE P.The protective role of microRNA-140-5p in synovial injury of rats with knee osteoarthritis via inactivating the TLR4/Myd88/NF-κB signaling pathway[J].Cell Cycle, 2019, 18(18):2344-2358.
[19]	ZHOU M Q, SHAO L, WU J, et al.Dihydromyricetin protects against lipopolysaccharide-induced cardiomyocyte injury through the toll-like receptor-4/nuclear factor-κB pathway[J].Mol Med Rep, 2017, 16(6):8983-8988.
[20]	杨子江.低硒日粮对肉鸡免疫器官中硒蛋白与细胞因子表达的影响[D].哈尔滨:东北农业大学, 2016.YANG Z J.Effects of diet with low selenium on the selenoproteins and cytokines expressions in broilers immune organs[D].Harbin:Northeast Agricultural University, 2016.(in Chinese)
[21]	梁娜, 彭西, 吴邦元, 等.亚硒酸钠对黄曲霉毒素B1致雏鸡胸腺细胞死亡受体通路活化的缓解作用研究[J].四川农业大学学报, 2018, 36(4):527-534.LIANG N, PENG X, WU B Y, et al.Protective effects of dietary sodium selenite on the activation of death receptor pathway in thymus of the chicks exposed to AFB1[J].Journal of Sichuan Agricultural University, 2018, 36(4):527-534.(in Chinese)
[22]	郭凤英, 李培锋, 贾俊涛.牛磺胆酸对小鼠胸腺细胞凋亡影响的研究[J].湖北农业科学, 2017, 56(19):3706-3707, 3713.GUO F Y, LI P F, JIA J T.The studies on taurocholic acid influencing mice thymocyte apoptosis[J].Hubei Agricultural Sciences, 2017, 56(19):3706-3707, 3713.(in Chinese)
[23]	XU S, XIAOJING L, XINYUE S, et al.Pig lung fibrosis is active in the subacute CdCl2 exposure model and exerts cumulative toxicity through the M1/M2 imbalance[J].Ecotoxicol Environ Saf, 2021, 225:112757.
[24]	王云.鸡胸腺发育形态学研究[D].武汉:华中农业大学, 2010.WANG Y.Morphological study on the development of chicken thymus[D].Wuhan:Huazhong Agricultural University, 2010.(in Chinese)
[25]	王巧红.硒缺乏致鸡免疫抑制机制的研究[D].哈尔滨:东北农业大学, 2009.WANG Q H.Research on the mechanism of selenium deficiency-induced immunosuppression in chickens[D].Harbin:Northeast Agricultural University, 2009.(in Chinese)
[26]	PAN T R, LIU T Q, TAN S R, et al.Lower selenoprotein T expression and immune response in the immune organs of broilers with exudative diathesis due to selenium deficiency[J].Biol Trace Elem Res, 2018, 182(2):364-372.
[27]	彭西.日粮硒对雏鸡免疫功能影响的机理研究[D].雅安:四川农业大学, 2009.PENG X.Mechanisms of the immune functional impairment caused by dietary selenium in chicks[D].Ya'an:Sichuan Agricultural University, 2009.(in Chinese)
[28]	KHOSO P A, YANG Z J, LIU C P, et al.Selenium deficiency downregulates selenoproteins and suppresses immune function in chicken thymus[J].Biol Trace Elem Res, 2015, 167(1):48-55.
[29]	LIANG Y, LIN S L, WANG C W, et al.Effect of selenium on selenoprotein expression in the adipose tissue of chickens[J].Biol Trace Elem Res, 2014, 160(1):41-48.
[30]	WANG Q Y, HUANG J Q, ZHANG H, et al.Selenium deficiency-induced apoptosis of chick embryonic vascular smooth muscle cells and correlations with 25 selenoproteins[J].Biol Trace Elem Res, 2017, 176(2):407-415.
[31]	YU D, ZHANG Z W, YAO H D, et al.The role of selenoprotein W in inflammatory injury in chicken immune tissues and cultured splenic lymphocyte[J].Biometals, 2015, 28(1):75-87.
[32]	WANG S C, ZHAO X, LIU Q Q, et al.Selenoprotein K protects skeletal muscle from damage and is required for satellite cells-mediated myogenic differentiation[J].Redox Biol, 2022, 50:102255.
[33]	DIWAKAR B T, KORWAR A M, PAULSON R F, et al.The regulation of pathways of inflammation and resolution in immune cells and cancer stem cells by selenium[J].Adv Cancer Res, 2017, 136:153-172.
[34]	ROYET J.Infectious non-self recognition in invertebrates:lessons from Drosophila and other insect models[J].Mol Immunol, 2004, 41(11):1063-1075.
[35]	秦洪琼.桂枝麻黄各半汤对流感病毒FM1株感染寒环境中小鼠肺部TLR7和RLH信号通路的影响[D].广州:暨南大学, 2018.QIN H Q.Effects of Guizhimahuanggebantang on the TLR7 and RLH pathway in influenza virus infected mouse lungs in a cold environment[D].Guangzhou:Ji'nan University, 2018.(in Chinese)
[36]	SHOU Y, LI N Y, LI L, et al.NF-kappaB-mediated up-regulation of Bcl-X(S) and Bax contributes to cytochrome c release in cyanide-induced apoptosis[J].J Neurochem, 2002, 81(4):842-852.
[37]	GILL J S, WINDEBANK A J.Ceramide initiates NF kappaB-mediated caspase activation in neuronal apoptosis[J].Neurobiol Dis, 2000, 7(4):448-461.
[38]	FEI X W, HE Y T, CHEN J, et al.The role of Toll-like receptor 4 in apoptosis of brain tissue after induction of intracerebral hemorrhage[J].J Neuroinflammation, 2019, 16(1):234.

Selenium Deficiency Induced Thymocytes Apoptosis of Broilers via the TLR4/MyD88/NF-κB Signaling Pathway

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