硒缺乏通过TLR4/MyD88/NF-κB信号通路介导鸡胸腺细胞凋亡

doi:10.11843/j.issn.0366-6964.2022.11.029

Abstract

Abstract: This study aimed to investigate whether selenium deficiency induced apoptosis of chicken thymocytes by activating toll-like receptor signaling pathway. In vivo experiment, 200 healthy one-day-old broilers were randomly divided into control group (C group) and selenium deficiency group (L group) by replicating selenium deficiency model, the control group was fed a normal diet with 0.2 mg·kg^-1 selenium, and the se-deficient group was fed a se-deficient diet with 0.004 mg·kg^-1 selenium. At 15, 25, 35, 45, and 55 days of age, 15 chicks were selected from each group, the morphological and ultrastructural changes of thymus tissue were observed. TLR4 signaling pathway and apoptosis-related factors were detected in thymus tissue. The selenium deficiency model of MDCC-MSB1(MSB1) cells in vitro was established, and six groups were established on this basis:Control (C) group, Selenium deficiency (L) group, selenium deficiency+transfection empty plasmid (L+pCMV-HA-N) group, selenium deficiency+siRNA negative control (L+ NCsiRNA) group, selenium deficiency + overexpression of TLR4 (L+ pCMV-HA-TLR4) group, selenium deficiency +siChTLR4 (L+ siChTLR4) group. After 5 days of low selenium treatment, cell viability, apoptosis, TLR4 signal transduction pathway and expression of apoptosis-related factors were detected. Results were as follows:1) Compared with the C group, the number of lymphocytes in the medulla of the cortex in the L group was reduced, the cells were disordered, the medulla of the cortex was hyperemic, the nucleus was fragmented, and extensive focal necrosis was observed. The thymus tissue of chickens in L group showed fissure, reduced volume, cell fragmentation, and nuclear chromatin edge aggregation, mitochondrial swelling, cristae fracture; 2) Compared with C group at 15 days of age, mRNA and protein expressions of TLR4, MyD88, NF-κB, Caspase-3, Caspase-9 and Bax in the thymus of L group were up-regulated at 15 to 55 days of age, the expression levels of Bcl-2 mRNA and protein showed opposite trends; 3) Compared with C group, the viability of MSB1 cells in L group was significantly decreased, the number of apoptosis was significantly increased, the mRNA and protein expressions of TLR4, MyD88, NF-κB, Caspase-3, Caspase-9 and Bax were significantly increased, and the mRNA and protein expression levels of Bcl-2 were significantly decreased. Compared with the L group, the cell viability was significantly enhanced, the number of apoptosis was significantly decreased, and the mRNA and protein expression of related factors were significantly decreased in the L+siChTLR4 group, while the cell viability was significantly decreased, the number of apoptosis was significantly increased, and the mRNA and protein expression of related factors were significantly increased in the L+ pCMV-HA-TLR4 group. In conclusion, selenium deficiency mediates the apoptosis of chicken thymocyte through the TLR4/MyD88/NF-κB signaling pathway, and further induces thymus injury in chickens.

Key words: selenium deficiency, TLR4, MyD88, NF-κB, thymus, apoptosis

CLC Number:

S852.3

ZHANG Ruili, ZHANG Di, GUO Rong, CHEN Yang, LI Guangxing, HUANG Xiaodan. Selenium Deficiency Induced Thymocytes Apoptosis of Broilers via the TLR4/MyD88/NF-κB Signaling Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4035-4047.

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Selenium Deficiency Induced Thymocytes Apoptosis of Broilers via the TLR4/MyD88/NF-κB Signaling Pathway

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