多房棘球蚴诱导小鼠枯否氏细胞极化相关ceRNA调控网络的构建与分析

doi:10.11843/j.issn.0366-6964.2022.12.026

Abstract

Abstract: Echinococcus multilocularis is a severe zoonotic parasite. To explore the mechanism of non-coding RNA in the polarization of mice liver Kupffer cells (KCs) induced by E. multilocularis infection, we herein infected the BALB/c mouse with E. multilocularis and isolated the KCs from the mice liver at 2 months post infection (Mpi). After RNA high-throughput sequencing, we analyzed and constructed the ceRNA regulatory network related to the M2 polarization of KCs induced by E. multilocularis infection. The qPCR and miRNA overexpression assays were used to verify the ceRNA regulatory network. The results showed that, after E. multilocularis infection 2 Mpi, the M1-type markers were significantly down-regulated, while the M2-type markers were significantly up-regulated. Among them, 5 circRNAs and 9 miRNAs were identified to be involved in regulating the expression of M2-type markers, such as IL10, IL13, and Arg1, and their expression patterns were consistent with the sequencing results and the circRNA-miRNA-mRNA regulatory relationship. Moreover, overexpression of miR-466c-5p led to the down-regulation of circ_0000372 and IL13, indicating that the circ_0000372-miR-466c-5p-IL13 axis plays vital role in the polarization of mouse liver macrophages induced by E. multilocular infection. This study provides an essential theoretical basis for further revealing the mechanism of E. multilocularis regulating the polarization of host macrophages.

Key words: Echinococcus multilocularis, polarization of M2-type macrophages, ceRNA, regulation networks

CLC Number:

S852.734

LI Yanping, LIU Tingli, LI Hong, CHEN Guoliang, WANG Liqun, GUO Xiaola, LUO Xuenong. Construction and Analysis of Polarization-related ceRNA Regulatory Network in Mouse Kupffer Cells Induced by Echinococcus multilocularis[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4410-4418.

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Construction and Analysis of Polarization-related ceRNA Regulatory Network in Mouse Kupffer Cells Induced by Echinococcus multilocularis

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