鸡环状RNA circSESN1的表达特性探究

doi:10.11843/j.issn.0366-6964.2022.07.011

Abstract

Abstract: The purpose of this study was to identify the expression of circSESN1 in chickens and to further explore the expression characteristics of circSESN1 in chicken pectoral muscle. In experiment 1, the expression of circRNA of chicken SESN1(circSESN1) was identified by RT-PCR, Sanger sequencing, and qRT-PCR. Then the spatio-temporal expression characteristics of circSESN1 and its source gene SESN1 were detected by qRT-PCR with tissue samples of silky fowls at different growth stages (E10, E19, 21 d, 49 d, n=4). In experiment 2, the effects of exogenous stimuli on the expression of circSESN1 were explored with the pectoral muscle of broilers collected at different time points after intraperitoneal injection of glucose (18 d, n=6), sodium pyruvate (21 d, n=6) or insulin (24 d, n=6). In experiment 3, 21 d (n=10) broilers were conducted with 15% energy restriction and 15% protein restriction, and the effect of feeding restriction on the expression of circSESN1 was explored in the pectoral muscle tissue of broilers. In addition, the potential function of circSESN1 was predicted by the bioinformatics analysis software. The results showed as follows:1) circSESN1 was a circular RNA with high stability in chicken and can not be digested by RNase R enzyme. 2) The relative expression levels of circSESN1 and source gene SESN1 were the highest in the pectoralis of 21 d silky fowl, followed by leg muscle and pancreas (P<0.01). The expression level of circSESN1 dramatically varied in the pectoral muscle of birds at different growth stages, with the highest level at 21 d and the lowest at E10 (P<0.01). The expression of SESN1 was highest in the pectoral muscle of silky fowl at E19 and the lowest at E10 (P<0.01). 3) After insulin injection, the expression of circSESN1 in the pectoral muscle showed a trend of first increasing and then decreasing at different time points. CircSESN1 was significantly higher at 120 min after insulin injection than the basal state or PBS group (P<0.01). 4) Compared with the control group, the expression of circSESN1 decreased significantly by 15% energy restriction and 15% protein restriction in the pectoral muscle of birds (P<0.05). 5) After predicting the potential function of circSESN1, it was found that circSESN1 functioned as efficient miRNA sponges, but can not encode proteins. The above results showed that circSESN1 was expressed in chicken, and it had similar spatio-temporal expression characteristics with its source gene SESN1. Exogenous insulin and feeding restriction can regulate the expression of circSESN1 in the pectoral muscle.

Key words: circular RNA, circSESN1, feed restriction, insulin, chicken

CLC Number:

S831.2

SHAO Binghao, GAO Linge, ZHU Xinghao, ZHANG Huaiyong, CHEN Wen, HUANG Yanqun. Expression Characteristics of Circular RNA circSESN1 in Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2141-2151.

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Expression Characteristics of Circular RNA circSESN1 in Chicken

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