鸭干扰素诱导的跨膜蛋白抑制基因3型鸭甲型肝炎病毒的增殖

doi:10.11843/j.issn.0366-6964.2022.03.015

Abstract

Abstract: In order to investigate the changes of expression levels of duck interferon-induced transmembrane proteins (duIFITMs) and related cytokines in the early stage of duck hepatitis A virus genotype 3 (DHAV-3) infection, and to evaluate the inhibitory effect of duIFITMs against proliferation of DHAV-3, the high-throughput sequencing and real-time PCR were used to evaluate the expression levels of duIFITMs and their related cytokines in the liver of ducklings at the early stage by the infection of DHAV-3. The cell models of DELC with duIFITM1 over-expression or knockdown were constructed by transfection of pEGFP-duIFITM1 and duIFITM1-siRNA, respectively. Then, the effect of duIFITM1 on DHAV-3 proliferation was evaluated. The results showed that duIFITM1 was significantly up-regulated at 24 and 36 h after DHAV-3 infection in ducklings (P<0.01), but duIFITM5 had no change in the early stage of DHAV-3 infection. Then dulIFITM1 over-expressing and knockdown DELC were infected with DHAV-3. Compared with control group and siRNA interference group, the number of DHAV-3 copies and virus titers decreased in the duIFITM1 overexpression group significantly at both 48 and 60 h (P<0.01). Furthermore, a total of 211 molecules and 74 enriched signaling pathways, which related to immune response against DHAV-3 were founded by transcriptome analyses. The results of real-time PCR detection confirmed that the levels of mRNA of RIG-I and MDA5 had no changes in livers at 12 and 24 h after inoculated by DHAV-3, but it increased to 4.23 times (P<0.01) and 3.61 times (P<0.05) in 36 h compared with the control group, these expression changes of RLRs coincided with that of IFN-α/IFN-β in early stage of DHAV-3 infection. Besides, the expression levels of IRF1 and IRF3 were upregulated significantly in early stage. It was the first time to be demonstrated that the duIFITM1 have an inhibitory effect against DHAV-3 proliferation, which could provide theoretical foundations for the development of new anti-DHAV drugs. Systematic study and analysis of the expression levels of a variety of key immune molecules in the early stage of anti-viral infection might be an important reference for the further exploration of the molecular mechanisms of DHAV-3 infection and immunity.

Key words: interferon-inducible transmembrane protein, duck hepatitis A virus, transcriptome, cytokine, overexpression, RNA interference

CLC Number:

S852.659.6

WANG Yidan, CHEN Dishi, XIANG Hua, ZHANG Huanrong, REN Yupeng. Inhibitory Effect of Duck Interferon-induced Transmembrane Proteins against Proliferation of DHAV-3[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 822-833.

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Inhibitory Effect of Duck Interferon-induced Transmembrane Proteins against Proliferation of DHAV-3

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