Abstract: The purpose of this study was to detect the expression of PPP1R3C in different tissues of AA broilers and to explore the effects of exogenous insulin and energy restriction on the expression of PPP1R3C in insulin sensitive tissues of chickens. In experiment 1, tissue samples of female broilers at different developmental stages (E14, E19, D7 and D21, n=10) were taken to detect the expression of PPP1R3C in chest muscle by qRT-PCR technique; In experiment 2, the tissue samples of D24 male broilers at different time points (0, 15, 120 and 240 min, n=5) after intraperitoneal injection of insulin or PBS were taken, to detect the expression of PPP1R3C in different tissues of broilers, and to explore the effect of exogenous insulin treatment on the expression of PPP1R3C in insulin sensitive tissues of broilers; In experiment 3, one group of D18 female broilers was fed with conventional diet (n=20) and the other group was fed diet with 30% energy restriction (n=20). After feeding for 48 days, slaughtering samples were taken to explore the effect of 30% energy restriction on the expression of PPP1R3C in broilers; In experiment 4, D7 female broilers were randomly divided into 3 groups:control group, 15% energy restriction group and 15% protein restriction group (n=10). Slaughtering samples were taken from D21 female broilers to explore whether energy restriction was dose-dependent. The results showed that:1) PPP1R3C was highly expressed in chest muscle, followed by heart and leg muscle(P<0.05). 2) The expression of PPP1R3C showed a increasing trend with the growth and development of chicken. 3) Insulin injection significantly down-regulated the expression of PPP1R3C in chest muscle, and the expression of PPP1R3C at 120 min after insulin injection was significantly lower than that at 0 and 15 min(P<0.05); There was no significant change in the expression of PPP1R3C in chest muscle after PBS injection, the expression of PPP1R3C in INS group was significantly lower than that in PBS group at 120 and 240 min after injection(P<0.05). Insulin injection also decreased the expression of PPP1R3C in liver, and the expression of PPP1R3C after insulin treatment for 15 min was significantly lower than at 0 min (P<0.05); The expression of PPP1R3C in liver was in dynamic balance after PBS injection, at 120 min after injection, the expression of PPP1R3C in INS group was significantly lower than that in PBS group(P<0.05). The expression of PPP1R3C in abdominal fat was opposite to that in chest muscle and liver after insulin injection, the expression of PPP1R3C at 15 min after insulin injection was significantly higher than that at 0,120 and 240 min(P<0.05), but there was no significant difference among 0, 120, 240 min; There was no significant change in the expression of PPP1R3C in abdominal fat after PBS injection, the expression of PPP1R3C in INS group was significantly higher than that in PBS group at 15 min after injection (P<0.05). 4) The expression of PPP1R3C in chest muscle and liver was significantly down-regulated by 30% energy restriction (P<0.05). 5) 15% energy restriction and 15% protein restriction did not significantly affect the expression of PPP1R3C in chest muscle. The study results showed that the expression of PPP1R3C was the highest in the chest muscle, and increased with the development of broilers, and the effect of exogenous insulin on the expression of PPP1R3C had obvious tissue specificity. At the same time, the results of energy restriction showed that 30% energy restriction could produce an effect similar to that of exogenous insulin, and the energy restriction had a certain dose dependence, which laid a foundation for further revealing the function of chicken PPP1R3C.

Key words: PPP1R3C, chicken, energy restriction, insulin