冷应激后民猪背脂中差异lncRNA的筛选与分析

doi:10.11843/j.issn.0366-6964.2021.04.009

Abstract

Abstract: The study aimed to analyze the effect of cold stress on the expression of lncRNA in adipose tissue of pigs, and discuss the effects of lncRNA and their target genes change on fat meta-bolism and body. In this study, six 6-month-old female Min pigs were randomly divided into two groups. The first group was raised in a normal house and the temperature was controlled at (18±2) ℃ and set as the control group. The second group was raised in an outdoor semi-convertible house with the temperature controlled at (-18±5) ℃ and set as the experimental group. After 24 h of treatment, the subcutaneous fat of back was taken as the experimental material after slaughter. lncRNAs and their target genes induced by low temperature were screened out through transcriptomic sequencing, and the biological function analysis of the differentially expressed genes was carried out, the regulatory network between them was constructed. The results showed that, 166 differentially expressed lncRNAs and 269 genes were induced by cold stress, of which only 2 were known lncRNAs. Among the differentially expressed genes, some genes were related to immune system, such as interferon stimulating genes, DHX58, PTX3 and OASL, some genes were related to respiration, oxidative stress and blood circulation, such as KCNK3, CCDC38, GAS2L2, KNDC1, LIPG and C1QL3, and some genes were related to lipid metabolism, such as ANGPTL4, KLF11, NUPR1 and HERC5, these genes were all significantly up-regulated. However, some genes related to nervous system, such as SLIT1, MAP6, TREM2 and IRFN2, were significantly down-regulated. The differentially expressed genes mainly involved in defense response, response to biological stimulation and response to virus. One hundred and forty four lncRNAs were found coexpression with 208 target genes by correlation coefficient analysis, the main accommodation mode between them was trans-regulation, there was a complex regulatory relationship between them. The screened target genes were significantly enriched in the ribosome biogenesis in eukaryotes and basal cell carcinoma pathways. It is speculated that the innate immune system, respiration, oxidative stress, blood circulation and sensitivity of nervous system were all affected by cold stress, and lipid metabolism was changed.

Key words: Min pig, lncRNA, mRNA, cold stress, regulatory network

CLC Number:

S828.2

MA Junjie, WANG Liang, LIU Di, ZHANG Dongjie, YANG Xiuqin. Screening and Analysis of Differentially Expressed lncRNA in Min Pig Back Fat after Cold Stress[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 932-942.

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Screening and Analysis of Differentially Expressed lncRNA in Min Pig Back Fat after Cold Stress

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