LncRNA NR_003508通过海绵吸附miR-483-3p并靶向MLKL调控BCG感染小鼠巨噬细胞坏死

doi:10.11843/j.issn.0366-6964.2022.09.029

Abstract

Abstract: To investigate the function of LncRNA NR_003508 in regulating Bacillus Calmette-Guérin (BCG)-induced necrosis of mouse macrophages, small interfering RNA for LncRNA NR_003508 were transfected into RAW264.7 cells alone or combined with BCG infection. Western blot, immunofluorescence and flow cytometry were used to detect the expression level of MLKL (mixed lineage kinase domain-like protein) and cell necrosis rate; The target binding of LncRNA NR_003508 and miR-483-3p was predicted and verified by bioinformatics and dual-luciferase report assay. The results showed that after BCG infection of macrophages, the expression level of LncRNA NR_003508 was significantly increased (P<0.01); After transfection of siRNA-LncRNA NR_003508, the protein expression of MLKL was significantly down-regulated (P<0.01), and the rate of macrophage necrosis was also significantly reduced (P<0.05). LncRNA NR_003508 adsorbed miR-483-3p through sponge effect, and interference of LncRNA NR_003508 significantly up-regulated the expression of miR-483-3p (P<0.01), while overexpression of miR-483-3p significantly inhibited the expression of MLKL (P<0.01) and macrophage necrosis. Our results indicated that LncRNA NR_003508 promoted BCG-induced macrophages necrosis by sponging miR-483-3p and targeting the expression of MLKL.

Key words: LncRNA NR_003508, miR-483-3p, BCG, cell necrosis, mixed lineage kinase domain-like protein

CLC Number:

S852.618

YU Zhirui, ZHANG Xu, NIU Shasha, DENG Guangcun, WU Xiaoling. LncRNA NR_003508 Regulates BCG-infected Mouse Macrophages Necrosis by the Sponge Adsorption of miR-483-3p and Targeting MLKL[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3149-3159.

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LncRNA NR_003508 Regulates BCG-infected Mouse Macrophages Necrosis by the Sponge Adsorption of miR-483-3p and Targeting MLKL

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