牛亚科物种TRs分布特点及着丝粒区卫星DNA进化研究

doi:10.11843/j.issn.0366-6964.2021.010.010

Abstract

Abstract: The objective of this study was to investigate the distribution characterization of tandem repeats sequence (TRs) and the evolution of centromeric satellite DNA in bovinae. In this study, based on the genome sequences of 6 bovine subfamily species including Bos taurus, Bos indicus, Bos mutus, Bubalus bubalis, Bison bison and Bos frontalis, the composition, distribution and structural characteristics of TRs among different species were studied, and the evolutionary relationships of centromeric satellite DNA were analyzed. The results showed that:1) The average percentage and length of TRs in the 6 bovine subfamily species were 2.03% and 54.93 Mb, resepctviely. Of which the highest percentage and length was Bos taurus (3.42%, 93.00 Mb), the lowest was Bos indicus (1.42%, 37.88 Mb). 2) Among the 3 types of tandem repeats, microsatellite DNA had the largest number(483 405), accounting for 85.64% of the total TRs, which was higher than minisatellites (43 026, 7.62%) and satellite sequences (38 180, 6.75%). 3) Through the analysis of the abundance and average length of microsatellite DNA, the dinucleo-tide of microsatellite DNA had the highest abundance in bovine subfamily, which was 70.93 loci/Mb and dominated by AC copy types. 4) 1.715 satellite sequence generally present in the genomes of bovinae species by constructing phylogenetic trees of 1.715 and 1.723 satellite sequences, while the 1.723 sequence was lacking in yaks. The two types of satellite sequences had different degrees of differentiation between different species or different chromosomes, which had obvious interspecies specificity. The average proportion of TRs in the 6 species of bovine subfamily was 2.03%. Microsatellite DNA was the main composition of TRs, the abundance of dinucleotide microsatellites was the highest, and the AC copy type was dominant. 1.715 satellite DNA generally existed in the genomes of 6 bovine subfamily species, but there were different degrees of differentiation among species or chromosomes. The results of this study provide an theoretical basis for the study of TRs distribution characteristics and evolutionary relationships among bovinae species.

Key words: bovinae, tandem repeats sequence, satellite DNA, evolution

CLC Number:

S823.2

ZHANG Rui, MA Jun, CHEN Yan, ZHANG Tianliu, FAN Tingting, ZHU Bo, ZHANG Lupei, XU Lingyang, GAO Huijiang, LI Junya, GAO Xue. Characterization and Distribution of TRs and Evolution of Centromeric Satellite DNA in Bovinae[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2792-2802.

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Characterization and Distribution of TRs and Evolution of Centromeric Satellite DNA in Bovinae

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