牛腺病毒3型感染状况及其fiber轴区基因检测

doi:10.11843/j.issn.0366-6964.2023.03.043

Abstract

Abstract: This study was conducted to investigate the infection status of BAdV-3 by TB Green Real-time PCR on 140 nasal swab samples of sick cattle characterized by runny nose, fever, cough and dyspnea from 9 large-scale cattle farms in 6 provinces in China (Sichuan Province, Shanxi Province, Henan Province, Hebei Province, Jiangsu Province and Inner Mongolia Autonomous Region), and the positive samples were tested for the fiber shaft gene. The results showed that the BAdV-3 positive rate was 36.4% among 140 samples, and BAdV-3 was detected in all provinces except for Hebei Province, with the highest detection rates in Sichuan and Henan Provinces, reaching 82.9% (29/35) and 83.3% (10/12), respectively. Among them, the traditional types accounted for 80.4% of the total positive ratio, and the strain with the deletion of the fiber shaft region accounted for 19.6% of the total positive samples. The position and number of deletions were consistent, with 237 consecutive base pairs missing in the shaft region, and the deletion type was only detected in Sichuan and Henan Provinces, with a detection rate of 24.1% (7/29) and 30% (3/10), and a field group positive rate of 100% (2/2) and 100% (1/1), respectively. The results indicate that BAdV-3 has a wide distribution in cattle farms in China, and the current epidemic virus is mainly the traditional strain of BAdV-3. The fiber shaft gene deletion strain has a unique geographical distribution in China and is mainly prevalent in Sichuan and Henan Provinces.

Key words: bovine adenovirus type 3, epidemiological investigation, real-time PCR, genetics and evolution, the gene deletion in the fiber shaft region

CLC Number:

S852.659.1

HE Shufan, ZOU Yuantong, HUANG Zhilan, LI Qian, JIANGCUO Wengxi, YUE Hua, TANG Cheng, LIU Jie. Detection of Bovine Adenovirus Type 3 Infection Status and Its Fiber Shaft Gene[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1333-1340.

References 34

[1]	ZHANG J, WANG W, YANG M J, et al. Development of a one-step multiplex real-time PCR assay for the detection of viral pathogens associated with the bovine respiratory disease complex[J]. Front Vet Sci, 2022, 9:825257.
[2]	AUTIO T, POHJANVIRTA T, HOLOPAINEN R, et al. Etiology of respiratory disease in non-vaccinated, non-medicated calves in rearing herds[J]. Vet Microbiol, 2007, 119(2-4):256-265.
[3]	MATTSON D E. Naturally occurring infection of calves with a bovine adenovirus[J]. Am J Vet Res, 1973, 34(5):623-629.
[4]	NARITA M, YAMADA M, TSUBOI T, et al. Immunohistopathology of calf pneumonia induced by endobronchial inoculation with bovine adenovirus 3[J]. Vet Pathol, 2002, 39(5):565-571.
[5]	何姝凡,岳华,汤承,等.牛腺病毒3型的研究进展[J].畜牧兽医学报, 2022, 53(4):1030-1040.HE S F, YUE H, TANG C, et al. Research advances in bovine adenovirus type 3[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4):1030-1040.(in Chinese)
[6]	NIIYAMA Y, IGARASHI K, TSUKAMOTO K, et al. Biochemical studies on bovine adenovirus type 3.I. Purification and properties[J]. J Virol, 1975, 16(3):621-633.
[7]	杨萌.牛腺病毒ELISA检测方法的建立及在咸阳地牛腺病毒血清流行病学调查中的应用[D].杨凌:西北农林科技大学, 2015.YANG M. The establishment of an ELISA method for serological investigation the epidemiology of bovine adenovirus in Xianyang area[D]. Yangling:Northwest A&F University, 2015.(in Chinese)
[8]	GINSBERG H S, PEREIRA H G, VALENTINE R C, et al. A proposed terminology for the adenovirus antigens and virion morphological subunits[J]. Virology, 1966, 28(4):782-783.
[9]	IZADPANAH R, BENKÖ M, URSU K, et al. Characterisation of the fiber gene and partial sequence of the early region 4 of bovine adenovirus 2(short communication)[J]. Acta Vet Hung, 2001, 49(2):245-252.
[10]	BANGARI D S, SHARMA A, MITTAL S K. Bovine adenovirus type 3 internalization is independent of primary receptors of human adenovirus type 5 and porcine adenovirus type 3[J]. Biochem Biophys Res Commun, 2005, 331(4):1478-1484.
[11]	WU E, PACHE L, VON SEGGERN D J, et al. Flexibility of the adenovirus fiber is required for efficient receptor interaction[J]. J Virol, 2003, 77(13):7225-7235.
[12]	SHAYAKHMETOV D M, LIEBER A. Dependence of adenovirus infectivity on length of the fiber shaft domain[J]. J Virol, 2000, 74(22):10274-10286.
[13]	WU Q H, TIKOO S K. Altered tropism of recombinant bovine adenovirus type-3 expressing chimeric fiber[J]. Virus Res, 2004, 99(1):9-15.
[14]	AMBRIOVIĆ-RISTOV A, MERCIER S, ELOIT M. Shortening adenovirus type 5 fiber shaft decreases the efficiency of postbinding steps in CAR-expressing and nonexpressing cells[J]. Virology, 2003, 312(2):425-433.
[15]	SEKI T, DMITRIEV I, SUZUKI K, et al. Fiber shaft extension in combination with HI loop ligands augments infectivity for CAR-negative tumor targets but does not enhance hepatotropism in vivo[J]. Gene Ther, 2002, 9(16):1101-1108.
[16]	DARBYSHIRE J H, JENNINGS A R, OMAR A R, et al. Association of adenoviruses with bovine respiratory disease[J]. Nature, 1965, 208(5007):307-308.
[17]	LEHMKUHL H D, HOBBS L A. Serologic and hexon phylogenetic analysis of ruminant adenoviruses[J]. Arch Virol, 2008, 153(5):891-897.
[18]	BAPTISTA A L, REZENDE A L, DE ALMEIDA FONSECA P, et al. Bovine respiratory disease complex associated mortality and morbidity rates in feedlot cattle from southeastern Brazil[J]. J Infect Dev Ctries, 2017, 11(10):791-799.
[19]	BAUER D J, CONDY J B. Isolation and characterisation of bovine adenoviruses types 3, 4 and 8 from free-living African buffaloes (Syncerus caffer)[J]. Res Vet Sci, 1981, 31(1):69-75.
[20]	SHEN Y, LIU J, ZHANG Y H, et al. Prevalence and characteristics of a novel bovine adenovirus type 3 with a natural deletion fiber gene[J]. Infect Genet Evol, 2020, 83:104348.
[21]	YESILBAČ K, GÜNGÖR B. Seroprevalence of bovine respiratory viruses in North-Western Turkey[J]. Trop Anim Health Prod, 2008, 40(1):55-60.
[22]	YEŞILBAǦ K, ALPAY G, KARAKUZULU H. A serologic survey of viral infections in captive ungulates in Turkish zoos[J]. J Zoo Wildl Med, 2011, 42(1):44-48.
[23]	PARDON B, DE BLEECKER K, DEWULF J, et al. Prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves[J]. Vet Rec, 2011, 169(11):278.
[24]	ROSHTKHARI F, MOHAMMADI G, MAYAMEEI A. Serological evaluation of relationship between viral pathogens (BHV-1, BVDV, BRSV, PI-3V, and Adeno3) and dairy calf pneumonia by indirect ELISA[J]. Trop Anim Health Prod, 2012, 44(5):1105-1110.
[25]	HÄRTEL H, NIKUNEN S, NEUVONEN E, et al. Viral and bacterial pathogens in bovine respiratory disease in Finland[J]. Acta Vet Scand, 2004, 45(3-4):193-200.
[26]	KRZYSIAK M K, JABȽOŃSKI A, IWANIAK W, et al. Seroprevalence and risk factors for selected respiratory and reproductive tract pathogen exposure in European bison (Bison bonasus) in Poland[J]. Vet Microbiol, 2018, 215:57-65.
[27]	NG T F F, KONDOV N O, DENG X T, et al. A metagenomics and case-control study to identify viruses associated with bovine respiratory disease[J]. J Virol, 2015, 89(10):5340-5349.
[28]	于作,朱远茂,蔡红,等.一株3型牛腺病毒的分离与鉴定[J].中国预防兽医学报, 2011, 33(3):169-172.YU Z, ZHU Y M, CAI H, et al. Isolation and identification of bovine adenovirus serotype 3[J]. Chinese Journal of Preventive Veterinary Medicine, 2011, 33(3):169-172.(in Chinese)
[29]	ZHU Y M, YU Z, CAI H, et al. Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China[J]. Virol J, 2011, 8:557.
[30]	严昊.牛腺病毒3型六邻体蛋白单抗制备及双抗夹心ELISA的建立与应用[D].北京:中国农业科学院, 2014.YAN H. Preparation of monoclonal antibodies against Hexon protein, establishment and application of a double antibody sandwich ELISA for detecton of bovine adenovirus type 3[D]. Beijing:ChineseAcademy of Agricultural Sciences, 2014.(in Chinese)
[31]	史鸿飞.牛腺病毒3型和牛副流感病毒3型对实验动物致病性的研究[D].北京:中国农业科学院, 2014.SHI H F. The pathogenicity of bovine adenovirus type 3 and bovine parainfluenza type 3 to laboratory animals[D]. Beijing:Chinese Academy of Agricultural Sciences, 2014.(in Chinese)
[32]	FULTON R W, DOWNING M M, HAGSTAD H V. Prevalence of bovine herpesvirus-1, bovine oral diarrhea, parainfluenza-3, bovine adenoviruses-3 and-7, and goat respiratory syncytial viral antibodies in goats[J]. Am J Vet Res, 1982, 43(8):1454-1457.
[33]	KAROL G M, RAÚL R AA, LENIN M H, et al. Association of bacterial and viral agents in acute pneumonia in young alpacas[J]. Rev Investig Vet Perú, 2013, 24(4):524-536.
[34]	ÇERIBASI A O,ÇERIBASI S, OZKARACA M. Immunohistochemical detection of bovine herpesvirus type 1 and bovine adenovirus type 3 antigens in frozen and paraffinized lung sections of pneumonic sheep and goats[J]. Vet Arhiv, 2016, 86(1):9-21.

Detection of Bovine Adenovirus Type 3 Infection Status and Its Fiber Shaft Gene

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